Restriction map for
pF1KA1291
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# NO CUT ENZYMES ( 80 enzymes) #
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AatII AccIII AclI AcyI AflII
AgeI AocI ApaI AscI AspI
AviII BbeI BbrPI BcgI BfrI
BglII BsaHI BsaI BseAI BsiEI
BsiWI BspDI BspEI BspLU11I BspMI
BsrBI BssHII BssSI Bsu36I ClaI
CpoI DsaI EagI EclXI Eco52I
Eco81I EcoRV FauI FseI FspI
Hin1I KasI KspI MfeI MluI
MroI MunI NaeI NarI NcoI
NgoMIV NotI NruI PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshAI Psp1406I PvuI RsrII SacI
SacII SalI SbfI ScaI SexAI
SfiI SfoI SgrAI SnaBI SpeI
Sse8387I SwaI Tth111I XbaI XhoI
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# ONE CUT ENZYMES ( 59 enzymes) #
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Acc65I 3320 GGTACC G^GTACC
AccI 2173 GTATAC GT^[AC][GT]AC
AflIII 216 ACACGT A^C[GA][CT]GT
AhdI 706 GACTGGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 2153 GTGCAC G^TGCAC
ApaLI 2153 GTGCAC G^TGCAC
AseI 3084 ATTAAT AT^TAAT
AsnI 3084 ATTAAT AT^TAAT
Asp718I 3320 GGTACC G^GTACC
AspEI 706 GACTGGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 2550 CCTAGG C^CTAGG
BanII 1127 GAGCCC G[GA]GC[CT]^C
BbvCI 3014 CCTCAGC CCTCAGC(-5/-2)
BciVI 2734 GTATCC GTATCC(6/5)
BclI 368 TGATCA T^GATCA
BglI 2377 GCCAAAATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 2550 CCTAGG C^CTAGG
BlpI 2621 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 2621 GCTCAGC GC^T[ACGT]AGC
BsaAI 3207 TACGTG [CT]AC^GT[GA]
BsaBI 459 GATGTTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 848 ACCGGA [AT]^CCGG[AT]
Bsp1407I 3073 TGTACA T^GTACA
BspHI 56 TCATGA T^CATGA
BsrFI 86 ACCGGC [GA]^CCGG[CT]
BsrGI 3073 TGTACA T^GTACA
Bst1107I 2173 GTATAC GTA^TAC
BstBI 1553 TTCGAA TT^CGAA
BstEII 3006 GGTCACC G^GT[ACGT]ACC
BstPI 3006 GGTCACC G^GT[ACGT]ACC
BstZ17I 2173 GTATAC GTA^TAC
CelII 2621 GCTCAGC GC^T[ACGT]AGC
Cfr10I 86 ACCGGC [GA]^CCGG[CT]
DraIII 2056 CACAGAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 2473 GACTCTCCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 706 GACTGGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 3006 GGTCACC G^GT[ACGT]ACC
EcoRI 1437 GAATTC G^AATTC
EcoT22I 886 ATGCAT ATGCA^T
FbaI 368 TGATCA T^GATCA
HindIII 3394 AAGCTT A^AGCTT
HpaI 2892 GTTAAC GTT^AAC
KpnI 3320 GGTACC GGTAC^C
MamI 459 GATGTTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NdeI 2770 CATATG CA^TATG
NheI 2373 GCTAGC G^CTAGC
NsiI 886 ATGCAT ATGCA^T
NspV 1553 TTCGAA TT^CGAA
PpuMI 2597 AGGACCT [GA]G^G[AT]CC[CT]
PshBI 3084 ATTAAT AT^TAAT
RcaI 56 TCATGA T^CATGA
SfuI 1553 TTCGAA TT^CGAA
SmaI 1781 CCCGGG CCC^GGG
SphI 1806 GCATGC GCATG^C
SspBI 3073 TGTACA T^GTACA
StuI 2124 AGGCCT AGG^CCT
XcmI 1202 CCATGACTAACTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 1781 CCCGGG C^CCGGG
XmaI 1781 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AccII 21 CGCG CG^CG
2421 CGCG
AcsI 1437 GAATTC [GA]^AATT[CT]
1574 AAATTC
Aor51HI 80 AGCGCT AGC^GCT
3078 AGCGCT
ApoI 1437 GAATTC [GA]^AATT[CT]
1574 AAATTC
BanI 2019 GGCACC G^G[CT][GA]CC
3320 GGTACC
BcnI 1781 CCCGG CC^[GC]GG
1782 CCGGG
BsmBI 2978 GAGACG CGTCTC(1/5)
3559 GAGACG
BsmI 2504 GAATGC GAATGC(1/-1)
2180 GCATTC
BstAPI 907 GCACAGACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2055 GCACAGAGTGC
BstUI 21 CGCG CG^CG
2421 CGCG
DraI 118 TTTAAA TTT^AAA
3399 TTTAAA
Eco47III 80 AGCGCT AGC^GCT
3078 AGCGCT
HincII 2632 GTCAAC GT[CT]^[GA]AC
2892 GTTAAC
HindII 2632 GTCAAC GT[CT]^[GA]AC
2892 GTTAAC
MvnI 21 CGCG CG^CG
2421 CGCG
NciI 1781 CCCGG CC^[GC]GG
1782 CCGGG
PvuII 34 CAGCTG CAG^CTG
994 CAGCTG
SspI 1259 AATATT AAT^ATT
2644 AATATT
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# 3 OR MORE CUT ENZYMES ( 101 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
Asp700I AspHI AvaI AvaII BalI
BamHI BbsI BbvI BfaI BmyI
BpmI BpuAI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BsrDI BsrI BssKI
BstF5I BstNI BstXI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraII
EaeI EarI EcoNI EcoO109I EcoRII
EcoT14I Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MwoI NdeII
NlaIII NlaIV NspI PflMI PleI
PstI RsaI SapI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XhoII
XmnI