Restriction map for
pF1KA1180
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# NO CUT ENZYMES ( 130 enzymes) #
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AatII AccI AccII AccIII AclI
AcsI AflII AgeI AhdI Alw44I
Aor51HI ApaI ApaLI ApoI AscI
AseI AsnI Asp700I AspEI AspI
AvaI AviII AvrII BamHI BbsI
BcgI BciVI BclI BfrI BglI
BglII BlnI BlpI Bpu1102I BpuAI
BsaWI BseAI BsiEI BsiWI BsmBI
BsmFI BsmI BsoBI BspDI BspEI
BspHI BsrBI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstYI BstZ17I CelII ClaI CpoI
DraI DraII DrdI EagI Eam1105I
EclXI Eco47III Eco52I EcoO109I EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
FspI HindIII HpaI KspI MfeI
MflI MluI MroI MunI MvnI
NaeI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmeI PpuMI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacI SacII SalI SbfI ScaI
SfiI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspI
SwaI TfiI Tsp509I Tth111I XbaI
XhoI XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 57 enzymes) #
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Acc65I 248 GGTACC G^GTACC
AflIII 608 ACATGT A^C[GA][CT]GT
AlwI 63 GATCC GGATC(4/5)
AlwNI 782 CAGACTCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 241 CCTCAGG CC^T[ACGT]AGG
Asp718I 248 GGTACC G^GTACC
AspHI 493 GTGCTC G[AT]GC[AT]^C
BalI 359 TGGCCA TGG^CCA
BanII 69 GGGCTC G[GA]GC[CT]^C
BbeI 340 GGCGCC GGCGC^C
BbrPI 199 CACGTG CAC^GTG
BcnI 66 CCGGG CC^[GC]GG
BfaI 473 CTAG C^TAG
BpmI 788 CTGGAG CTGGAG(16/14)
BsaAI 199 CACGTG [CT]AC^GT[GA]
BsaBI 324 GATTGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 626 GAGACC GGTCTC(1/5)
BsiHKAI 493 GTGCTC G[AT]GC[AT]^C
Bsp1407I 611 TGTACA T^GTACA
BspLU11I 608 ACATGT A^CATGT
BspMI 590 ACCTGC ACCTGC(4/8)
BsrDI 864 CATTGC GCAATG(2/0)
BsrGI 611 TGTACA T^GTACA
BstXI 962 CCAGCTCGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 241 CCTCAGG CC^T[ACGT]AGG
CfoI 341 GCGC GCG^C
DraIII 184 CACTTTGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 359 TGGCCA [CT]^GGCC[GA]
Eco81I 241 CCTCAGG CC^T[ACGT]AGG
EcoNI 387 CCTGGTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HaeII 340 GGCGCC [GA]GCGC^[CT]
HhaI 341 GCGC GCG^C
HinP1I 341 GCGC G^CGC
HincII 1030 GTCAAC GT[CT]^[GA]AC
HindII 1030 GTCAAC GT[CT]^[GA]AC
HinfI 784 GACTC G^A[ACGT]TC
KasI 340 GGCGCC G^GCGCC
KpnI 248 GGTACC GGTAC^C
MaeI 473 CTAG C^TAG
MamI 324 GATTGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 359 TGGCCA TGG^CCA
MscI 359 TGGCCA TGG^CCA
MseI 638 TTAA T^TAA
NarI 340 GGCGCC GG^CGCC
NciI 66 CCGGG CC^[GC]GG
PleI 784 GACTC GAGTC(4/5)
PmaCI 199 CACGTG CAC^GTG
PmlI 199 CACGTG CAC^GTG
PstI 592 CTGCAG CTGCA^G
PvuII 271 CAGCTG CAG^CTG
SapI 597 GCTCTTC GCTCTTC(1/4)
SexAI 386 ACCTGGT A^CC[AT]GGT
SfcI 592 CTGCAG C^T[GA][CT]AG
SfoI 340 GGCGCC GGC^GCC
SspBI 611 TGTACA T^GTACA
StuI 989 AGGCCT AGG^CCT
Tru9I 638 TTAA T^TAA
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# TWO CUT ENZYMES ( 24 enzymes) #
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AcyI 228 GGCGTC G[GA]^CG[CT]C
340 GGCGCC
AvaII 750 GGACC G^G[AT]CC
879 GGACC
BbvCI 908 CCTCAGC CCTCAGC(-5/-2)
715 GCTGAGG
BsaHI 228 GGCGTC G[GA]^CG[CT]C
340 GGCGCC
BseRI 517 GAGGAG GAGGAG(10/8)
896 GAGGAG
BsmAI 34 GAGAC GTCTC(1/5)
626 GAGAC
BsrFI 554 ACCGGC [GA]^CCGG[CT]
641 ACCGGC
Cfr10I 554 ACCGGC [GA]^CCGG[CT]
641 ACCGGC
DpnII 63 GATC ^GATC
174 GATC
DsaI 263 CCATGG C^C[GA][CT]GG
1040 CCATGG
EarI 505 CTCTTC CTCTTC(1/4)
598 CTCTTC
HgaI 669 GACGC GACGC(5/10)
229 GCGTC
Hin1I 228 GGCGTC G[GA]^CG[CT]C
340 GGCGCC
Ksp632I 505 CTCTTC CTCTTC(1/4)
598 CTCTTC
MaeII 200 ACGT A^CGT
572 ACGT
MaeIII 197 GTCAC ^GT[ACGT]AC
805 GTCAC
MboI 63 GATC ^GATC
174 GATC
NcoI 263 CCATGG C^CATGG
1040 CCATGG
NdeII 63 GATC ^GATC
174 GATC
PflMI 108 CCATGATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1035 CCACACCATGG
Sau3AI 63 GATC ^GATC
174 GATC
Tsp45I 197 GTCAC ^GT[GC]AC
805 GTCAC
Van91I 108 CCATGATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1035 CCACACCATGG
XcmI 179 CCAAGCACTTTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
263 CCATGGAGCAGCTGG
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# 3 OR MORE CUT ENZYMES ( 47 enzymes) #
#########################################
AciI AfaI AluI BanI BbvI
BmyI BsaJI BsgI BsiYI BslI
Bsp1286I BsrI BssKI BstF5I BstNI
Cac8I Cfr13I DdeI EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HpaII HphI ItaI MboII MnlI
MslI MspA1I MspI MvaI MwoI
NlaIII NlaIV NspI RsaI Sau96I
ScrFI SfaNI StyI TaqI TseI
TspRI TthHB8I