Restriction map for
pF1KA1122
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# NO CUT ENZYMES ( 122 enzymes) #
##################################
AatII AccI AccII AccIII AciI
AclI AcyI AgeI AhdI Alw44I
AocI Aor51HI ApaLI AscI AspEI
AspHI AspI AvaI AviII BamHI
BbeI BbrPI BbvCI BcgI BciVI
BcnI BglI BglII BlpI Bpu1102I
BsaBI BsaHI BsaI BsaWI BseAI
BsgI BsiEI BsiHKAI BsiWI BsmBI
BsoBI BspDI BspEI BsrBI BsrFI
BssHII BssSI Bst1107I BstEII BstPI
BstUI BstYI BstZ17I Bsu36I CelII
Cfr10I ClaI CpoI DraI DrdI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoO65I EcoRI FauI FseI
FspI HgaI Hin1I HincII HindII
HpaI KasI KspI MamI MflI
MluI MroI MvnI NaeI NarI
NciI NgoMIV NheI NotI NruI
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI Psp1406I PvuI
RsrII SacI SacII SalI SapI
SbfI SexAI SfiI SfoI SgrAI
SmaI SnaBI SphI Sse8387I SwaI
Tth111I Van91I XbaI XhoI XhoII
XmaCI XmaI
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# ONE CUT ENZYMES ( 54 enzymes) #
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Acc65I 2693 GGTACC G^GTACC
AflII 532 CTTAAG C^TTAAG
AlwNI 1085 CAGGTTCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 610 GGGCCC GGGCC^C
Asp718I 2693 GGTACC G^GTACC
AvrII 1575 CCTAGG C^CTAGG
BalI 2348 TGGCCA TGG^CCA
BclI 672 TGATCA T^GATCA
BfrI 532 CTTAAG C^TTAAG
BlnI 1575 CCTAGG C^CTAGG
BpmI 212 CTCCAG CTGGAG(16/14)
BsaAI 416 CACGTA [CT]AC^GT[GA]
BseRI 124 GAGGAG GAGGAG(10/8)
BsiYI 1614 CCTCTATCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 1614 CCTCTATCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI 2406 GTCTC GTCTC(1/5)
BsmI 129 GAATGC GAATGC(1/-1)
BspHI 2327 TCATGA T^CATGA
BspMI 601 GCAGGT ACCTGC(4/8)
BstAPI 568 GCAATTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 2488 TTCGAA TT^CGAA
BstXI 2645 CCATGATGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI 58 GCGC GCG^C
DraII 1715 AGGTCCT [GA]G^G[ACGT]CC[CT]
DsaI 410 CCATGG C^C[GA][CT]GG
EaeI 2348 TGGCCA [CT]^GGCC[GA]
EcoNI 1614 CCTCTATCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 1715 AGGTCCT [GA]G^G[ACGT]CC[CT]
EcoT22I 2586 ATGCAT ATGCA^T
FbaI 672 TGATCA T^GATCA
HaeII 57 AGCGCC [GA]GCGC^[CT]
HapII 1254 CCGG C^CGG
HhaI 58 GCGC GCG^C
HinP1I 58 GCGC G^CGC
HindIII 851 AAGCTT A^AGCTT
HpaII 1254 CCGG C^CGG
KpnI 2693 GGTACC GGTAC^C
MluNI 2348 TGGCCA TGG^CCA
MscI 2348 TGGCCA TGG^CCA
MspA1I 2384 CAGCTG C[AC]G^C[GT]G
MspI 1254 CCGG C^CGG
NcoI 410 CCATGG C^CATGG
NsiI 2586 ATGCAT ATGCA^T
NspV 2488 TTCGAA TT^CGAA
PpuMI 1715 AGGTCCT [GA]G^G[AT]CC[CT]
PshAI 1837 GACCGTAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 725 CTGCAG CTGCA^G
PvuII 2384 CAGCTG CAG^CTG
RcaI 2327 TCATGA T^CATGA
SfuI 2488 TTCGAA TT^CGAA
SpeI 2922 ACTAGT A^CTAGT
SspI 2375 AATATT AAT^ATT
StuI 399 AGGCCT AGG^CCT
XcmI 1595 CCATTGCATTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AflIII 2030 ACATGT A^C[GA][CT]GT
2496 ACATGT
AlwI 1344 GATCC GGATC(4/5)
699 GGATC
AseI 1939 ATTAAT AT^TAAT
2524 ATTAAT
AsnI 1939 ATTAAT AT^TAAT
2524 ATTAAT
BanI 1700 GGTGCC G^G[CT][GA]CC
2693 GGTACC
BanII 610 GGGCCC G[GA]GC[CT]^C
1908 GGGCTC
BsmFI 2947 GGGAC GGGAC(10/14)
393 GTCCC
Bsp1407I 842 TGTACA T^GTACA
1977 TGTACA
BspLU11I 2030 ACATGT A^CATGT
2496 ACATGT
BsrGI 842 TGTACA T^GTACA
1977 TGTACA
Cac8I 1521 GCTGGC GC[ACGT]^[ACGT]GC
3042 GCCAGC
DraIII 905 CACAAAGTG CAC[ACGT][ACGT][ACGT]^GTG
2432 CACATTGTG
EcoRV 2656 GATATC GAT^ATC
2767 GATATC
MfeI 569 CAATTG C^AATTG
1190 CAATTG
MunI 569 CAATTG C^AATTG
1190 CAATTG
NdeI 647 CATATG CA^TATG
1891 CATATG
PshBI 1939 ATTAAT AT^TAAT
2524 ATTAAT
ScaI 819 AGTACT AGT^ACT
2919 AGTACT
SspBI 842 TGTACA T^GTACA
1977 TGTACA
#########################################
# 3 OR MORE CUT ENZYMES ( 63 enzymes) #
#########################################
AcsI AfaI AluI ApoI Asp700I
AvaII BbsI BbvI BfaI BmyI
BpuAI BsaJI Bsp1286I BsrDI BsrI
BssKI BstF5I BstNI Cfr13I DdeI
DpnII EarI EcoRII EcoT14I Fnu4HI
FokI HaeIII HinfI HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MnlI MseI MslI MvaI
MwoI NdeII NlaIII NlaIV NspI
PleI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XmnI