Restriction map for
pF1KA1119
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# NO CUT ENZYMES ( 38 enzymes) #
##################################
AclI AscI AvrII BbeI BcgI
BciVI BlnI BsiEI BsiWI CpoI
DraIII EagI EclXI Eco52I EcoT22I
FseI HindIII KasI KspI MluI
NarI NdeI NheI NotI NruI
NsiI PacI PmeI Psp1406I PvuI
RsrII SacII SalI ScaI SfoI
SgrAI SnaBI XbaI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AccI 1066 GTATAC GT^[AC][GT]AC
AflII 4747 CTTAAG C^TTAAG
AgeI 2114 ACCGGT A^CCGGT
AhdI 359 GACAAGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 4922 AGCGCT AGC^GCT
AspEI 359 GACAAGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1170 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 41 GGATCC G^GATCC
BbrPI 2608 CACGTG CAC^GTG
BfrI 4747 CTTAAG C^TTAAG
BsaAI 2608 CACGTG [CT]AC^GT[GA]
Bsp1407I 159 TGTACA T^GTACA
BspDI 2800 ATCGAT AT^CGAT
BsrDI 638 GCAATG GCAATG(2/0)
BsrGI 159 TGTACA T^GTACA
BssHII 1198 GCGCGC G^CGCGC
BssSI 3754 CACGAG CACGAG(-5/-1)
Bst1107I 1066 GTATAC GTA^TAC
BstEII 1143 GGTCACC G^GT[ACGT]ACC
BstPI 1143 GGTCACC G^GT[ACGT]ACC
BstZ17I 1066 GTATAC GTA^TAC
ClaI 2800 ATCGAT AT^CGAT
DrdI 3427 GACATGACGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 359 GACAAGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 4922 AGCGCT AGC^GCT
EcoO65I 1143 GGTCACC G^GT[ACGT]ACC
EcoRV 196 GATATC GAT^ATC
HpaI 78 GTTAAC GTT^AAC
MfeI 152 CAATTG C^AATTG
MunI 152 CAATTG C^AATTG
NaeI 3832 GCCGGC GCC^GGC
NgoMIV 3832 GCCGGC G^CCGGC
PflFI 1170 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 2114 ACCGGT A^CCGGT
PmaCI 2608 CACGTG CAC^GTG
PmlI 2608 CACGTG CAC^GTG
PshAI 1158 GACCTACGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SbfI 5037 CCTGCAGG CCTGCA^GG
SexAI 4451 ACCTGGT A^CC[AT]GGT
SfiI 2481 GGCCCGCCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 2537 CCCGGG CCC^GGG
SpeI 1758 ACTAGT A^CTAGT
Sse8387I 5037 CCTGCAGG CCTGCA^GG
SspBI 159 TGTACA T^GTACA
SspI 3221 AATATT AAT^ATT
SwaI 3968 ATTTAAAT ATTT^AAAT
Tth111I 1170 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 2537 CCCGGG C^CCGGG
XmaI 2537 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 32 enzymes) #
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AatII 4638 GACGTC GACGT^C
5107 GACGTC
Acc65I 692 GGTACC G^GTACC
3200 GGTACC
AflIII 4520 ACATGT A^C[GA][CT]GT
5450 ACATGT
ApaI 2480 GGGCCC GGGCC^C
2981 GGGCCC
AseI 328 ATTAAT AT^TAAT
3856 ATTAAT
AsnI 328 ATTAAT AT^TAAT
3856 ATTAAT
Asp700I 133 GAAACGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4392 GAAGGATTTC
Asp718I 692 GGTACC G^GTACC
3200 GGTACC
AviII 2963 TGCGCA TGC^GCA
3770 TGCGCA
BanI 692 GGTACC G^G[CT][GA]CC
3200 GGTACC
BclI 1190 TGATCA T^GATCA
5066 TGATCA
BsaBI 1728 GATCAATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2058 GATTCGAATC
BsaI 1156 GAGACC GGTCTC(1/5)
1933 GAGACC
BsmBI 2752 CGTCTC CGTCTC(1/5)
2053 GAGACG
BspLU11I 4520 ACATGT A^CATGT
5450 ACATGT
BstAPI 1076 GCAACTTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
4566 GCACTCCCTGC
BstBI 1827 TTCGAA TT^CGAA
2060 TTCGAA
BstXI 2083 CCATCCAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4022 CCAGGCTGCTGG
EcoRI 4177 GAATTC G^AATTC
5530 GAATTC
FbaI 1190 TGATCA T^GATCA
5066 TGATCA
FspI 2963 TGCGCA TGC^GCA
3770 TGCGCA
HincII 78 GTTAAC GT[CT]^[GA]AC
3895 GTTGAC
HindII 78 GTTAAC GT[CT]^[GA]AC
3895 GTTGAC
KpnI 692 GGTACC GGTAC^C
3200 GGTACC
MamI 1728 GATCAATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2058 GATTCGAATC
NspV 1827 TTCGAA TT^CGAA
2060 TTCGAA
PaeR7I 3763 CTCGAG C^TCGAG
4096 CTCGAG
PshBI 328 ATTAAT AT^TAAT
3856 ATTAAT
SapI 1720 GAAGAGC GCTCTTC(1/4)
5055 GCTCTTC
SfuI 1827 TTCGAA TT^CGAA
2060 TTCGAA
XhoI 3763 CTCGAG C^TCGAG
4096 CTCGAG
XmnI 133 GAAACGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4392 GAAGGATTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 139 enzymes) #
#########################################
AccII AccIII AciI AcsI AcyI
AfaI AluI Alw44I AlwI AlwNI
AocI ApaLI ApoI AspHI AvaI
AvaII BalI BanII BbsI BbvCI
BbvI BcnI BfaI BglI BglII
BlpI BmyI BpmI Bpu1102I BpuAI
BsaHI BsaJI BsaWI BseAI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI BsoBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrFI BsrI
BssKI BstF5I BstNI BstUI BstYI
Bsu36I Cac8I CelII CfoI Cfr10I
Cfr13I DdeI DpnII DraI DraII
DsaI EaeI EarI Eco81I EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MroI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NcoI NdeII NlaIII NlaIV
NspI PflMI PleI PpuMI PstI
PvuII RcaI RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SphI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XcmI XhoII