Restriction map for
pF1KA0970
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# NO CUT ENZYMES ( 118 enzymes) #
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AatII Acc65I AccII AccIII AcyI
AgeI AhdI Aor51HI ApaI AscI
Asp718I AspEI AvaI AviII AvrII
BalI BbeI BbrPI BbvCI BcnI
BglI BlnI BlpI Bpu1102I BsaBI
BsaHI BseAI BsiEI BsiWI BsmBI
BsmI BsoBI BspDI BspEI BspHI
BspMI BsrBI BsrFI BssHII BssSI
Bst1107I BstUI BstXI BstZ17I CelII
CfoI Cfr10I ClaI CpoI DrdI
EagI Eam1105I EclXI Eco47III Eco52I
EcoNI EcoRV EcoT22I FauI FseI
FspI HaeII HgaI HhaI Hin1I
HinP1I HpaI KasI KpnI KspI
MamI MfeI MluI MluNI MroI
MscI MunI MvnI NaeI NarI
NciI NgoMIV NheI NotI NruI
NsiI PacI PaeR7I PflMI PinAI
PmaCI PmeI PmlI PshAI PstI
PvuI RcaI RsrII SacII SalI
SbfI SfiI SfoI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspI
StuI SwaI Van91I XbaI XcmI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 32 enzymes) #
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AccI 1847 GTAGAC GT^[AC][GT]AC
AclI 383 AACGTT AA^CGTT
AflII 2731 CTTAAG C^TTAAG
AflIII 2134 ACATGT A^C[GA][CT]GT
Alw44I 2153 GTGCAC G^TGCAC
ApaLI 2153 GTGCAC G^TGCAC
BamHI 1587 GGATCC G^GATCC
BciVI 2824 GTATCC GTATCC(6/5)
BfrI 2731 CTTAAG C^TTAAG
BsaAI 2036 TACGTG [CT]AC^GT[GA]
BsaWI 3140 TCCGGT [AT]^CCGG[AT]
Bsp1407I 1990 TGTACA T^GTACA
BspLU11I 2134 ACATGT A^CATGT
BsrGI 1990 TGTACA T^GTACA
BstAPI 2146 GCAACTTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 104 TTCGAA TT^CGAA
BstEII 689 GGTCACC G^GT[ACGT]ACC
BstPI 689 GGTCACC G^GT[ACGT]ACC
DsaI 851 CCATGG C^C[GA][CT]GG
EaeI 1163 CGGCCA [CT]^GGCC[GA]
EcoO65I 689 GGTCACC G^GT[ACGT]ACC
HapII 3141 CCGG C^CGG
HindIII 2903 AAGCTT A^AGCTT
HpaII 3141 CCGG C^CGG
MspI 3141 CCGG C^CGG
NcoI 851 CCATGG C^CATGG
NspV 104 TTCGAA TT^CGAA
Psp1406I 383 AACGTT AA^CGTT
SacI 742 GAGCTC GAGCT^C
SapI 3255 GCTCTTC GCTCTTC(1/4)
SfuI 104 TTCGAA TT^CGAA
SspBI 1990 TGTACA T^GTACA
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# TWO CUT ENZYMES ( 21 enzymes) #
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AseI 475 ATTAAT AT^TAAT
709 ATTAAT
AsnI 475 ATTAAT AT^TAAT
709 ATTAAT
AspI 2763 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
2840 GACCATGTC
BanI 129 GGCACC G^G[CT][GA]CC
1008 GGCACC
BanII 742 GAGCTC G[GA]GC[CT]^C
1098 GGGCTC
BcgI 1741 CGAGTTTACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
3342 CGAGCAGTGTGC
BclI 2490 TGATCA T^GATCA
3009 TGATCA
BglII 1395 AGATCT A^GATCT
2140 AGATCT
BsaI 1776 GGTCTC GGTCTC(1/5)
2263 GGTCTC
BseRI 182 CTCCTC GAGGAG(10/8)
2222 GAGGAG
BsrDI 999 GCAATG GCAATG(2/0)
1138 GCAATG
DraIII 1010 CACCTAGTG CAC[ACGT][ACGT][ACGT]^GTG
3332 CACTGAGTG
EcoRI 2612 GAATTC G^AATTC
3103 GAATTC
FbaI 2490 TGATCA T^GATCA
3009 TGATCA
HincII 2769 GTCAAC GT[CT]^[GA]AC
2877 GTCAAC
HindII 2769 GTCAAC GT[CT]^[GA]AC
2877 GTCAAC
NspI 2134 ACATGT [GA]CATG^[CT]
2458 ACATGC
PflFI 2763 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
2840 GACCATGTC
PshBI 475 ATTAAT AT^TAAT
709 ATTAAT
SexAI 189 ACCTGGT A^CC[AT]GGT
685 ACCTGGT
Tth111I 2763 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
2840 GACCATGTC
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# 3 OR MORE CUT ENZYMES ( 87 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI AocI ApoI Asp700I AspHI
AvaII BbsI BbvI BfaI BmyI
BpmI BpuAI BsaJI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BsrI BssKI BstF5I BstNI BstYI
Bsu36I Cac8I Cfr13I DdeI DpnII
DraI DraII EarI Eco81I EcoO109I
EcoRII EcoT14I Fnu4HI FokI HaeIII
HinfI HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MvaI
MwoI NdeI NdeII NlaIII NlaIV
PleI PpuMI PvuII RsaI Sau3AI
Sau96I ScaI ScrFI SfaNI SfcI
SmlI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII XmnI