Restriction map for
pF1KA0905
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# NO CUT ENZYMES ( 97 enzymes) #
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AatII AccI AccII AccIII AcyI
AflII AflIII Alw44I AocI ApaI
ApaLI AscI AseI AsnI AspI
AvaI AviII AvrII BamHI BbeI
BbrPI BcgI BclI BfrI BlnI
BsaAI BsaBI BsaHI BseAI BsiEI
BsmBI BsoBI Bsp1407I BspDI BspEI
BspLU11I BsrBI BsrGI BssHII Bst1107I
BstUI BstZ17I Bsu36I ClaI CpoI
DraI DrdI EagI EclXI Eco52I
Eco81I EcoRI FauI FbaI FseI
FspI Hin1I HpaI KasI KspI
MamI MluI MroI MvnI NaeI
NarI NgoMIV NheI NotI NruI
PacI PaeR7I PflFI PmaCI PmeI
PmlI PshAI PshBI PvuI RsrII
SacII SalI SbfI SexAI SfiI
SfoI SgrAI SmaI SnaBI Sse8387I
SspBI SwaI Tth111I XbaI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 37 enzymes) #
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AclI 3443 AACGTT AA^CGTT
AgeI 707 ACCGGT A^CCGGT
AhdI 3088 GACCCCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 3383 AGCGCT AGC^GCT
AspEI 3088 GACCCCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbsI 3147 GTCTTC GAAGAC(2/6)
BglII 1494 AGATCT A^GATCT
BpuAI 3147 GTCTTC GAAGAC(2/6)
BsaI 3598 GAGACC GGTCTC(1/5)
BsaWI 707 ACCGGT [AT]^CCGG[AT]
BsiWI 2408 CGTACG C^GTACG
BspHI 3071 TCATGA T^CATGA
BsrFI 707 ACCGGT [GA]^CCGG[CT]
BssSI 1454 CTCGTG CACGAG(-5/-1)
BstBI 1169 TTCGAA TT^CGAA
CfoI 3384 GCGC GCG^C
Cfr10I 707 ACCGGT [GA]^CCGG[CT]
Eam1105I 3088 GACCCCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 3383 AGCGCT AGC^GCT
EcoNI 2377 CCTGTAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 508 GATATC GAT^ATC
HaeII 3383 AGCGCT [GA]GCGC^[CT]
HgaI 2953 GCGTC GACGC(5/10)
HhaI 3384 GCGC GCG^C
HinP1I 3384 GCGC G^CGC
HindIII 1042 AAGCTT A^AGCTT
NspV 1169 TTCGAA TT^CGAA
PinAI 707 ACCGGT A^CCGGT
PpuMI 2967 AGGTCCT [GA]G^G[AT]CC[CT]
Psp1406I 3443 AACGTT AA^CGTT
RcaI 3071 TCATGA T^CATGA
SacI 2876 GAGCTC GAGCT^C
SapI 1606 GAAGAGC GCTCTTC(1/4)
ScaI 2218 AGTACT AGT^ACT
SfuI 1169 TTCGAA TT^CGAA
SpeI 2215 ACTAGT A^CTAGT
SphI 3221 GCATGC GCATG^C
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# TWO CUT ENZYMES ( 24 enzymes) #
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Acc65I 203 GGTACC G^GTACC
2601 GGTACC
Asp700I 390 GAACATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1232 GAATGCCTTC
Asp718I 203 GGTACC G^GTACC
2601 GGTACC
BbvCI 1314 CCTCAGC CCTCAGC(-5/-2)
2683 CCTCAGC
BciVI 1480 GGATAC GTATCC(6/5)
2646 GTATCC
BcnI 573 CCGGG CC^[GC]GG
2521 CCGGG
BspMI 271 GCAGGT ACCTGC(4/8)
1810 GCAGGT
BstAPI 1338 GCAGGCTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3297 GCAGTCTTTGC
BstEII 710 GGTTACC G^GT[ACGT]ACC
3085 GGTGACC
BstPI 710 GGTTACC G^GT[ACGT]ACC
3085 GGTGACC
DraII 222 GGGGCCT [GA]G^G[ACGT]CC[CT]
2967 AGGTCCT
EcoO109I 222 GGGGCCT [GA]G^G[ACGT]CC[CT]
2967 AGGTCCT
EcoO65I 710 GGTTACC G^GT[ACGT]ACC
3085 GGTGACC
HincII 1036 GTTGAC GT[CT]^[GA]AC
1756 GTTGAC
HindII 1036 GTTGAC GT[CT]^[GA]AC
1756 GTTGAC
KpnI 203 GGTACC GGTAC^C
2601 GGTACC
MaeII 1216 ACGT A^CGT
3444 ACGT
MfeI 94 CAATTG C^AATTG
794 CAATTG
MunI 94 CAATTG C^AATTG
794 CAATTG
NciI 573 CCGGG CC^[GC]GG
2521 CCGGG
NdeI 444 CATATG CA^TATG
2587 CATATG
PflMI 578 CCACTGTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2446 CCAGTTGCTGG
Van91I 578 CCACTGTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2446 CCAGTTGCTGG
XmnI 390 GAACATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1232 GAATGCCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 100 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI AspHI AvaII BalI
BanI BanII BbvI BfaI BglI
BlpI BmyI BpmI Bpu1102I BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI Bsp1286I BsrDI
BsrI BssKI BstF5I BstNI BstXI
BstYI Cac8I CelII Cfr13I DdeI
DpnII DraIII DsaI EaeI EarI
EcoRII EcoT14I EcoT22I Fnu4HI FokI
HaeIII HapII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MwoI NcoI NdeII NlaIII NlaIV
NsiI NspI PleI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SspI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XcmI XhoII