Restriction map for
pF1KA0866
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# NO CUT ENZYMES ( 63 enzymes) #
##################################
Acc65I AclI AgeI Aor51HI AscI
Asp718I AviII AvrII BanI BbeI
BbrPI BlnI BsaBI BsiWI BspDI
BssHII Bst1107I BstEII BstPI BstZ17I
ClaI CpoI DraI DraIII DrdI
Eco47III EcoO65I EcoRI EcoRV EcoT22I
FseI FspI HindIII HpaI KasI
KpnI KspI MamI MfeI MluI
MunI NarI NheI NruI NsiI
PacI PinAI PmaCI PmeI PmlI
PshAI Psp1406I PvuI RsrII SacII
SbfI ScaI SfoI SgrAI SpeI
Sse8387I SspI SwaI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AatII 751 GACGTC GACGT^C
AccIII 5390 TCCGGA T^CCGGA
AflII 2623 CTTAAG C^TTAAG
Alw44I 1659 GTGCAC G^TGCAC
AocI 312 CCTGAGG CC^T[ACGT]AGG
ApaLI 1659 GTGCAC G^TGCAC
AseI 1159 ATTAAT AT^TAAT
AsnI 1159 ATTAAT AT^TAAT
BamHI 1367 GGATCC G^GATCC
BcgI 740 GCATCAACTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1304 GGATAC GTATCC(6/5)
BfrI 2623 CTTAAG C^TTAAG
BlpI 5304 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 5304 GCTCAGC GC^T[ACGT]AGC
BsaAI 345 TACGTA [CT]AC^GT[GA]
BseAI 5390 TCCGGA T^CCGGA
BspEI 5390 TCCGGA T^CCGGA
BspHI 2246 TCATGA T^CATGA
BspLU11I 419 ACATGT A^CATGT
BsrFI 2132 GCCGGC [GA]^CCGG[CT]
BstBI 4726 TTCGAA TT^CGAA
Bsu36I 312 CCTGAGG CC^T[ACGT]AGG
CelII 5304 GCTCAGC GC^T[ACGT]AGC
Cfr10I 2132 GCCGGC [GA]^CCGG[CT]
EagI 4235 CGGCCG C^GGCCG
EclXI 4235 CGGCCG C^GGCCG
Eco52I 4235 CGGCCG C^GGCCG
Eco81I 312 CCTGAGG CC^T[ACGT]AGG
MroI 5390 TCCGGA T^CCGGA
NaeI 2132 GCCGGC GCC^GGC
NdeI 1283 CATATG CA^TATG
NgoMIV 2132 GCCGGC G^CCGGC
NotI 4234 GCGGCCGC GC^GGCCGC
NspV 4726 TTCGAA TT^CGAA
PshBI 1159 ATTAAT AT^TAAT
RcaI 2246 TCATGA T^CATGA
SalI 415 GTCGAC G^TCGAC
SfiI 585 GGCCGTGGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI 4726 TTCGAA TT^CGAA
SnaBI 345 TACGTA TAC^GTA
SphI 491 GCATGC GCATG^C
XbaI 5815 TCTAGA T^CTAGA
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# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AccI 415 GTCGAC GT^[AC][GT]AC
4002 GTCTAC
AcsI 730 AAATTC [GA]^AATT[CT]
4354 AAATTT
AflIII 419 ACATGT A^C[GA][CT]GT
3998 ACGTGT
AhdI 1102 GACCAGGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3925 GACGTGGCGTC
ApoI 730 AAATTC [GA]^AATT[CT]
4354 AAATTT
AspEI 1102 GACCAGGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3925 GACGTGGCGTC
BglII 1391 AGATCT A^GATCT
5009 AGATCT
BsgI 3781 GTGCAG GTGCAG(16/14)
3801 GTGCAG
Bsp1407I 422 TGTACA T^GTACA
1967 TGTACA
BsrDI 2102 GCAATG GCAATG(2/0)
5624 GCAATG
BsrGI 422 TGTACA T^GTACA
1967 TGTACA
Eam1105I 1102 GACCAGGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3925 GACGTGGCGTC
NcoI 995 CCATGG C^CATGG
5747 CCATGG
SexAI 4292 ACCTGGT A^CC[AT]GGT
4544 ACCTGGT
SmaI 3821 CCCGGG CCC^GGG
4745 CCCGGG
SspBI 422 TGTACA T^GTACA
1967 TGTACA
StuI 2234 AGGCCT AGG^CCT
4481 AGGCCT
XmaCI 3821 CCCGGG C^CCGGG
4745 CCCGGG
XmaI 3821 CCCGGG C^CCGGG
4745 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 134 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI ApaI Asp700I AspHI
AspI AvaI AvaII BalI BanII
BbsI BbvCI BbvI BclI BcnI
BfaI BglI BmyI BpmI BpuAI
BsaHI BsaI BsaJI BsaWI BseRI
BsiEI BsiHKAI BsiYI BslI BsmAI
BsmBI BsmFI BsmI BsoBI Bsp1286I
BspMI BsrBI BsrI BssKI BssSI
BstAPI BstF5I BstNI BstUI BstXI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
EcoNI EcoO109I EcoRII EcoT14I FauI
FbaI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HincII HindII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV NspI PaeR7I PflFI
PflMI PleI PpuMI PstI PvuII
RsaI SacI SapI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI Tth111I TthHB8I Van91I
XcmI XhoI XhoII XmnI