Restriction map for
pF1KA0777
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# NO CUT ENZYMES ( 80 enzymes) #
##################################
AatII Acc65I AclI AcyI AflII
AgeI AscI AseI AsnI Asp718I
AspI AvrII BbeI BciVI BfrI
BglII BlnI BlpI Bpu1102I BsaBI
BsaHI BsaI BsiWI BspDI BsrDI
BssSI Bst1107I BstEII BstPI BstZ17I
CelII ClaI CpoI DraI EagI
EclXI Eco52I EcoNI EcoO65I EcoRV
EcoT22I FseI Hin1I HpaI KasI
KpnI KspI MamI MfeI MluI
MunI NarI NdeI NheI NotI
NruI NsiI PacI PaeR7I PflFI
PinAI PmeI PshBI Psp1406I PvuI
RsrII SacII SbfI ScaI SexAI
SfiI SfoI SgrAI SpeI Sse8387I
StuI SwaI Tth111I XbaI XhoI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AccII 1471 CGCG CG^CG
AocI 2676 CCTCAGG CC^T[ACGT]AGG
Aor51HI 2328 AGCGCT AGC^GCT
ApaI 226 GGGCCC GGGCC^C
AviII 120 TGCGCA TGC^GCA
BalI 746 TGGCCA TGG^CCA
BbrPI 1774 CACGTG CAC^GTG
BbsI 555 GTCTTC GAAGAC(2/6)
BcgI 83 GCACATCCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BpuAI 555 GTCTTC GAAGAC(2/6)
BsiEI 273 CGACCG CG[GA][CT]^CG
BsmBI 1122 CGTCTC CGTCTC(1/5)
BsmI 2420 GAATGC GAATGC(1/-1)
BspHI 3105 TCATGA T^CATGA
BsrBI 1691 CCGCTC CCGCTC(-3/-3)
BssHII 1470 GCGCGC G^CGCGC
BstAPI 2776 GCACAGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 1537 TTCGAA TT^CGAA
BstUI 1471 CGCG CG^CG
BstXI 2478 CCAAGACCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 2676 CCTCAGG CC^T[ACGT]AGG
DraIII 316 CACATGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1189 GACCCGAAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 746 TGGCCA [CT]^GGCC[GA]
Eco47III 2328 AGCGCT AGC^GCT
Eco81I 2676 CCTCAGG CC^T[ACGT]AGG
EcoRI 1534 GAATTC G^AATTC
FspI 120 TGCGCA TGC^GCA
HaeII 2328 AGCGCT [GA]GCGC^[CT]
HincII 271 GTCGAC GT[CT]^[GA]AC
HindII 271 GTCGAC GT[CT]^[GA]AC
HindIII 1899 AAGCTT A^AGCTT
MluNI 746 TGGCCA TGG^CCA
MscI 746 TGGCCA TGG^CCA
MspA1I 2466 CAGCTG C[AC]G^C[GT]G
MvnI 1471 CGCG CG^CG
NaeI 1700 GCCGGC GCC^GGC
NcoI 1256 CCATGG C^CATGG
NgoMIV 1700 GCCGGC G^CCGGC
NspV 1537 TTCGAA TT^CGAA
PmaCI 1774 CACGTG CAC^GTG
PmlI 1774 CACGTG CAC^GTG
PshAI 1316 GACACAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 2466 CAGCTG CAG^CTG
RcaI 3105 TCATGA T^CATGA
SalI 271 GTCGAC G^TCGAC
SfuI 1537 TTCGAA TT^CGAA
SnaBI 2746 TACGTA TAC^GTA
SphI 117 GCATGC GCATG^C
SspI 3112 AATATT AAT^ATT
XcmI 2475 CCACCAAGACCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AccI 271 GTCGAC GT^[AC][GT]AC
2668 GTCTAC
AccIII 241 TCCGGA T^CCGGA
2117 TCCGGA
AhdI 1585 GACATGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2503 GACCGTGAGTC
Asp700I 3109 GAAAATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3130 GAACCGTTTC
AspEI 1585 GACATGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2503 GACCGTGAGTC
BamHI 579 GGATCC G^GATCC
1909 GGATCC
BbvCI 2331 GCTGAGG CCTCAGC(-5/-2)
3010 GCTGAGG
BglI 1700 GCCGGCGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2052 GCCCAGAAGGC
BsaAI 1774 CACGTG [CT]AC^GT[GA]
2746 TACGTA
BsaWI 241 TCCGGA [AT]^CCGG[AT]
2117 TCCGGA
BseAI 241 TCCGGA T^CCGGA
2117 TCCGGA
BsgI 2269 CTGCAC GTGCAG(16/14)
2347 CTGCAC
Bsp1407I 383 TGTACA T^GTACA
1355 TGTACA
BspEI 241 TCCGGA T^CCGGA
2117 TCCGGA
BspLU11I 347 ACATGT A^CATGT
513 ACATGT
BsrFI 275 ACCGGC [GA]^CCGG[CT]
1700 GCCGGC
BsrGI 383 TGTACA T^GTACA
1355 TGTACA
BstYI 579 GGATCC [GA]^GATC[CT]
1909 GGATCC
Cfr10I 275 ACCGGC [GA]^CCGG[CT]
1700 GCCGGC
Eam1105I 1585 GACATGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2503 GACCGTGAGTC
HgaI 490 GCGTC GACGC(5/10)
3090 GCGTC
MflI 579 GGATCC [GA]^GATC[CT]
1909 GGATCC
MroI 241 TCCGGA T^CCGGA
2117 TCCGGA
SacI 3072 GAGCTC GAGCT^C
3181 GAGCTC
SapI 771 GAAGAGC GCTCTTC(1/4)
890 GCTCTTC
SmaI 1338 CCCGGG CCC^GGG
1505 CCCGGG
SmlI 3069 CTTGAG C^T[CT][GA]AG
3246 CTCAAG
SspBI 383 TGTACA T^GTACA
1355 TGTACA
XhoII 579 GGATCC [GA]^GATC[CT]
1909 GGATCC
XmaCI 1338 CCCGGG C^CCGGG
1505 CCCGGG
XmaI 1338 CCCGGG C^CCGGG
1505 CCCGGG
XmnI 3109 GAAAATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3130 GAACCGTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 95 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
Alw44I AlwI AlwNI ApaLI ApoI
AspHI AvaI AvaII BanI BanII
BbvI BclI BcnI BfaI BmyI
BpmI BsaJI BseRI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrI BssKI BstF5I BstNI
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI EarI EcoO109I EcoRII
EcoT14I FauI FbaI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MnlI
MseI MslI MspI MvaI MwoI
NciI NdeII NlaIII NlaIV NspI
PflMI PleI PpuMI PstI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I