Restriction map for
pF1KA0750
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# NO CUT ENZYMES ( 86 enzymes) #
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AccI AccIII AclI AflII AgeI
AhdI Aor51HI ApaI AscI AseI
AsnI AspEI AspI AvrII BbeI
BbsI BciVI BfrI BlnI BpuAI
BsaI BseAI BsiEI BsiWI BsmI
BspDI BspEI BspLU11I BsrDI BssHII
Bst1107I BstBI BstXI BstZ17I ClaI
CpoI DraI EagI Eam1105I EclXI
Eco47III Eco52I EcoT22I FseI HgaI
HindIII HpaI KasI KspI MfeI
MluI MroI MunI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PflFI PinAI
PmeI PshAI PshBI Psp1406I PvuI
RsrII SacII SalI SbfI ScaI
SfiI SfoI SfuI SgrAI SnaBI
SpeI SphI Sse8387I SspI SwaI
Tth111I
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# ONE CUT ENZYMES ( 43 enzymes) #
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AatII 649 GACGTC GACGT^C
AccII 1137 CGCG CG^CG
AcyI 649 GACGTC G[GA]^CG[CT]C
Alw44I 249 GTGCAC G^TGCAC
AocI 2926 CCTAAGG CC^T[ACGT]AGG
ApaLI 249 GTGCAC G^TGCAC
BamHI 1766 GGATCC G^GATCC
BcgI 2156 CGAATCAGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 2916 TGATCA T^GATCA
BglI 1206 GCCATTTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaBI 726 GATTGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 649 GACGTC G[GA]^CG[CT]C
BsaWI 2008 ACCGGA [AT]^CCGG[AT]
BsgI 882 CTGCAC GTGCAG(16/14)
Bsp1407I 2906 TGTACA T^GTACA
BspHI 2204 TCATGA T^CATGA
BsrBI 1153 GAGCGG CCGCTC(-3/-3)
BsrGI 2906 TGTACA T^GTACA
BssSI 1174 CTCGTG CACGAG(-5/-1)
BstEII 168 GGTGACC G^GT[ACGT]ACC
BstPI 168 GGTGACC G^GT[ACGT]ACC
BstUI 1137 CGCG CG^CG
Bsu36I 2926 CCTAAGG CC^T[ACGT]AGG
DrdI 928 GACAAAGGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 2926 CCTAAGG CC^T[ACGT]AGG
EcoNI 495 CCTATTCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 168 GGTGACC G^GT[ACGT]ACC
EcoRI 706 GAATTC G^AATTC
EcoRV 1546 GATATC GAT^ATC
FbaI 2916 TGATCA T^GATCA
HaeII 3046 AGCGCC [GA]GCGC^[CT]
Hin1I 649 GACGTC G[GA]^CG[CT]C
MamI 726 GATTGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MvnI 1137 CGCG CG^CG
PaeR7I 1492 CTCGAG C^TCGAG
RcaI 2204 TCATGA T^CATGA
SexAI 1570 ACCTGGT A^CC[AT]GGT
SmaI 1016 CCCGGG CCC^GGG
SspBI 2906 TGTACA T^GTACA
XbaI 558 TCTAGA T^CTAGA
XhoI 1492 CTCGAG C^TCGAG
XmaCI 1016 CCCGGG C^CCGGG
XmaI 1016 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 23 enzymes) #
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Acc65I 79 GGTACC G^GTACC
1409 GGTACC
Asp700I 2040 GAAGGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2542 GAAAAGATTC
Asp718I 79 GGTACC G^GTACC
1409 GGTACC
AviII 296 TGCGCA TGC^GCA
3292 TGCGCA
BbvCI 1923 CCTCAGC CCTCAGC(-5/-2)
2842 CCTCAGC
BfaI 121 CTAG C^TAG
559 CTAG
BglII 852 AGATCT A^GATCT
1516 AGATCT
BlpI 1821 GCTCAGC GC^T[ACGT]AGC
3042 GCTGAGC
Bpu1102I 1821 GCTCAGC GC^T[ACGT]AGC
3042 GCTGAGC
BsrFI 1334 ACCGGC [GA]^CCGG[CT]
2241 GCCGGT
CelII 1821 GCTCAGC GC^T[ACGT]AGC
3042 GCTGAGC
Cfr10I 1334 ACCGGC [GA]^CCGG[CT]
2241 GCCGGT
DraIII 258 CACCAAGTG CAC[ACGT][ACGT][ACGT]^GTG
1427 CACACTGTG
FspI 296 TGCGCA TGC^GCA
3292 TGCGCA
KpnI 79 GGTACC GGTAC^C
1409 GGTACC
MaeI 121 CTAG C^TAG
559 CTAG
NcoI 1217 CCATGG C^CATGG
2150 CCATGG
NspI 1118 GCATGT [GA]CATG^[CT]
1602 GCATGT
PflMI 1625 CCACATCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3361 CCACTTCTTGG
PpuMI 282 AGGACCC [GA]G^G[AT]CC[CT]
827 AGGACCT
StuI 89 AGGCCT AGG^CCT
2676 AGGCCT
Van91I 1625 CCACATCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3361 CCACTTCTTGG
XmnI 2040 GAAGGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2542 GAAAAGATTC
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# 3 OR MORE CUT ENZYMES ( 106 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI AlwNI ApoI AspHI AvaI
AvaII BalI BanI BanII BbrPI
BbvI BcnI BmyI BpmI BsaAI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI BsmBI BsmFI BsoBI Bsp1286I
BspMI BsrI BssKI BstAPI BstF5I
BstNI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HincII HindII HinfI HpaII
HphI ItaI Ksp632I MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV PleI PmaCI PmlI PstI
PvuII RsaI SacI SapI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XcmI
XhoII