Restriction map for
pF1KA0727
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# NO CUT ENZYMES ( 91 enzymes) #
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AatII Acc65I AccIII AclI AflIII
AgeI AhdI AlwNI Aor51HI ApaI
AscI AseI AsnI Asp718I AspEI
AviII AvrII BbeI BciVI BclI
BglI BglII BlnI BlpI Bpu1102I
BsaBI BseAI BsiEI BsiWI Bsp1407I
BspEI BspHI BspLU11I BsrBI BsrGI
BssHII Bst1107I BstXI BstZ17I CelII
CpoI EagI Eam1105I EclXI Eco47III
Eco52I EcoT22I FbaI FseI FspI
HpaI KasI KpnI KspI MamI
MluI MroI MslI NarI NdeI
NheI NotI NruI NsiI PacI
PinAI PmeI PpuMI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SgrAI SnaBI SpeI
Sse8387I SspBI SspI StuI SwaI
XbaI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AccI 1726 GTAGAC GT^[AC][GT]AC
AccII 2948 CGCG CG^CG
AflII 415 CTTAAG C^TTAAG
Alw44I 2928 GTGCAC G^TGCAC
ApaLI 2928 GTGCAC G^TGCAC
AspI 52 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaII 2739 GGACC G^G[AT]CC
BbrPI 2275 CACGTG CAC^GTG
BbvCI 3000 CCTCAGC CCTCAGC(-5/-2)
BcgI 1379 GCATGAATGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 415 CTTAAG C^TTAAG
BsaAI 2275 CACGTG [CT]AC^GT[GA]
BsaI 219 GAGACC GGTCTC(1/5)
BsaWI 950 ACCGGA [AT]^CCGG[AT]
BsmFI 2524 GTCCC GGGAC(10/14)
BsmI 2372 GCATTC GAATGC(1/-1)
BspDI 2600 ATCGAT AT^CGAT
BstUI 2948 CGCG CG^CG
ClaI 2600 ATCGAT AT^CGAT
DraI 837 TTTAAA TTT^AAA
DraIII 2221 CACGAGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 2776 GACCTCATTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 2388 CCTGCCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HincII 1324 GTTGAC GT[CT]^[GA]AC
HindII 1324 GTTGAC GT[CT]^[GA]AC
MfeI 185 CAATTG C^AATTG
MunI 185 CAATTG C^AATTG
MvnI 2948 CGCG CG^CG
NaeI 1817 GCCGGC GCC^GGC
NgoMIV 1817 GCCGGC G^CCGGC
NspI 2144 GCATGC [GA]CATG^[CT]
PaeR7I 566 CTCGAG C^TCGAG
PflFI 52 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 2220 CCACGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 2817 GAGTC GAGTC(4/5)
PmaCI 2275 CACGTG CAC^GTG
PmlI 2275 CACGTG CAC^GTG
PvuII 1237 CAGCTG CAG^CTG
SacI 701 GAGCTC GAGCT^C
SmaI 3010 CCCGGG CCC^GGG
SphI 2144 GCATGC GCATG^C
Tth111I 52 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 2220 CCACGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 422 CCAACTGTGTTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 566 CTCGAG C^TCGAG
XmaCI 3010 CCCGGG C^CCGGG
XmaI 3010 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 33 enzymes) #
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AcyI 1021 GACGCC G[GA]^CG[CT]C
2245 GGCGTC
Asp700I 1405 GAAATGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2850 GAATCATTTC
BamHI 1280 GGATCC G^GATCC
2661 GGATCC
BanI 1820 GGCACC G^G[CT][GA]CC
2131 GGCACC
BanII 701 GAGCTC G[GA]GC[CT]^C
2452 GGGCTC
BcnI 3010 CCCGG CC^[GC]GG
3011 CCGGG
BfaI 556 CTAG C^TAG
1723 CTAG
BsaHI 1021 GACGCC G[GA]^CG[CT]C
2245 GGCGTC
BseRI 784 GAGGAG GAGGAG(10/8)
2317 GAGGAG
BspMI 2387 ACCTGC ACCTGC(4/8)
479 GCAGGT
BsrDI 1356 CATTGC GCAATG(2/0)
1208 GCAATG
BsrFI 1817 GCCGGC [GA]^CCGG[CT]
2192 ACCGGC
BssSI 2221 CACGAG CACGAG(-5/-1)
1330 CTCGTG
BstAPI 1870 GCAGGATTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2404 GCAAAGGTTGC
BstBI 1508 TTCGAA TT^CGAA
2042 TTCGAA
BstEII 520 GGTGACC G^GT[ACGT]ACC
1668 GGTGACC
BstPI 520 GGTGACC G^GT[ACGT]ACC
1668 GGTGACC
Cfr10I 1817 GCCGGC [GA]^CCGG[CT]
2192 ACCGGC
DraII 2321 AGGCCCT [GA]G^G[ACGT]CC[CT]
2461 AGGGCCT
EcoO109I 2321 AGGCCCT [GA]G^G[ACGT]CC[CT]
2461 AGGGCCT
EcoO65I 520 GGTGACC G^GT[ACGT]ACC
1668 GGTGACC
Hin1I 1021 GACGCC G[GA]^CG[CT]C
2245 GGCGTC
HindIII 437 AAGCTT A^AGCTT
597 AAGCTT
MaeI 556 CTAG C^TAG
1723 CTAG
MspA1I 1237 CAGCTG C[AC]G^C[GT]G
1270 CAGCGG
NciI 3010 CCCGG CC^[GC]GG
3011 CCGGG
NcoI 1116 CCATGG C^CATGG
2241 CCATGG
NspV 1508 TTCGAA TT^CGAA
2042 TTCGAA
PstI 1219 CTGCAG CTGCA^G
2326 CTGCAG
SfcI 1219 CTGCAG C^T[GA][CT]AG
2326 CTGCAG
SfuI 1508 TTCGAA TT^CGAA
2042 TTCGAA
TfiI 2850 GAATC G^A[AT]TC
2982 GAATC
XmnI 1405 GAAATGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2850 GAATCATTTC
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# 3 OR MORE CUT ENZYMES ( 87 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AocI ApoI AspHI AvaI BalI
BbsI BbvI BmyI BpmI BpuAI
BsaJI BsgI BsiHKAI BsiYI BslI
BsmAI BsmBI BsoBI Bsp1286I BsrI
BssKI BstF5I BstNI BstYI Bsu36I
Cac8I CfoI Cfr13I DdeI DpnII
DsaI EaeI EarI Eco81I EcoRI
EcoRII EcoRV EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MspI MvaI MwoI NdeII
NlaIII NlaIV RsaI Sau3AI Sau96I
ScrFI SfaNI SmlI StyI TaqI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII