Restriction map for
pF1KA0720
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# NO CUT ENZYMES ( 79 enzymes) #
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AatII Acc65I AccIII AclI AflII
AgeI AscI AseI AsnI Asp700I
Asp718I AspI AviII AvrII BamHI
BcgI BciVI BfrI BglII BlnI
BsaBI BseAI BsiWI Bsp1407I BspDI
BspEI BspHI BsrGI Bst1107I BstBI
BstZ17I ClaI CpoI DraI EcoRI
EcoRV FseI FspI HincII HindII
HindIII HpaI KpnI KspI MamI
MfeI MroI MunI NaeI NdeI
NgoMIV NotI NruI NspV PacI
PaeR7I PflFI PinAI PmeI PshBI
Psp1406I PvuI RcaI RsrII SacII
SalI ScaI SexAI SfiI SfuI
SnaBI SpeI SspBI SspI SwaI
Tth111I XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 36 enzymes) #
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AccI 967 GTAGAC GT^[AC][GT]AC
Alw44I 2237 GTGCAC G^TGCAC
AlwI 1995 GGATC GGATC(4/5)
ApaLI 2237 GTGCAC G^TGCAC
BbvCI 1662 GCTGAGG CCTCAGC(-5/-2)
BclI 1238 TGATCA T^GATCA
BlpI 1596 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1596 GCTGAGC GC^T[ACGT]AGC
BsmI 391 GAATGC GAATGC(1/-1)
BspLU11I 782 ACATGT A^CATGT
BsrDI 504 CATTGC GCAATG(2/0)
BsrFI 1377 GCCGGT [GA]^CCGG[CT]
BssHII 1683 GCGCGC G^CGCGC
BstEII 2007 GGTGACC G^GT[ACGT]ACC
BstPI 2007 GGTGACC G^GT[ACGT]ACC
BstYI 1995 GGATCT [GA]^GATC[CT]
CelII 1596 GCTGAGC GC^T[ACGT]AGC
Cfr10I 1377 GCCGGT [GA]^CCGG[CT]
DraIII 1735 CACCACGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 32 GACTGCGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 2007 GGTGACC G^GT[ACGT]ACC
EcoT22I 169 ATGCAT ATGCA^T
FbaI 1238 TGATCA T^GATCA
MflI 1995 GGATCT [GA]^GATC[CT]
MluI 1745 ACGCGT A^CGCGT
MseI 3158 TTAA T^TAA
NheI 1363 GCTAGC G^CTAGC
NsiI 169 ATGCAT ATGCA^T
PflMI 2817 CCAGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SbfI 3013 CCTGCAGG CCTGCA^GG
SgrAI 1376 CGCCGGTG C[GA]^CCGG[CT]G
SphI 1751 GCATGC GCATG^C
Sse8387I 3013 CCTGCAGG CCTGCA^GG
Tru9I 3158 TTAA T^TAA
Van91I 2817 CCAGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 1995 GGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 33 enzymes) #
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AcsI 406 AAATTT [GA]^AATT[CT]
1858 GAATTT
Aor51HI 1407 AGCGCT AGC^GCT
1724 AGCGCT
ApoI 406 AAATTT [GA]^AATT[CT]
1858 GAATTT
BbeI 1374 GGCGCC GGCGC^C
1894 GGCGCC
BbrPI 1557 CACGTG CAC^GTG
1738 CACGTG
BbsI 1081 GAAGAC GAAGAC(2/6)
1668 GAAGAC
BglI 1032 GCCCCGGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1687 GCCAAGGAGGC
BpuAI 1081 GAAGAC GAAGAC(2/6)
1668 GAAGAC
BsaAI 1557 CACGTG [CT]AC^GT[GA]
1738 CACGTG
BsaWI 320 ACCGGA [AT]^CCGG[AT]
2586 TCCGGT
BsiEI 1784 CGGCCG CG[GA][CT]^CG
2595 CGGCCG
BsmBI 2737 CGTCTC CGTCTC(1/5)
1909 GAGACG
BsrBI 2598 CCGCTC CCGCTC(-3/-3)
1768 GAGCGG
BssSI 2708 CACGAG CACGAG(-5/-1)
2062 CTCGTG
BstXI 2160 CCAGGCCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2447 CCATGGTTGTGG
EagI 1784 CGGCCG C^GGCCG
2595 CGGCCG
EclXI 1784 CGGCCG C^GGCCG
2595 CGGCCG
Eco47III 1407 AGCGCT AGC^GCT
1724 AGCGCT
Eco52I 1784 CGGCCG C^GGCCG
2595 CGGCCG
KasI 1374 GGCGCC G^GCGCC
1894 GGCGCC
NarI 1374 GGCGCC GG^CGCC
1894 GGCGCC
PmaCI 1557 CACGTG CAC^GTG
1738 CACGTG
PmlI 1557 CACGTG CAC^GTG
1738 CACGTG
PpuMI 2093 GGGACCC [GA]G^G[AT]CC[CT]
2101 GGGTCCT
PshAI 2037 GACCAGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
2494 GACAGCGGTC
PstI 2269 CTGCAG CTGCA^G
3014 CTGCAG
SapI 324 GAAGAGC GCTCTTC(1/4)
1458 GCTCTTC
SfaNI 809 GCATC GCATC(5/9)
1799 GCATC
SfoI 1374 GGCGCC GGC^GCC
1894 GGCGCC
SmlI 1651 CTCAAG C^T[CT][GA]AG
2788 CTCAAG
TfiI 678 GATTC G^A[AT]TC
3137 GAATC
Tsp509I 407 AATT ^AATT
1859 AATT
XcmI 260 CCAGCCCCGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2432 CCACGGAGACCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 110 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AhdI AluI AlwNI AocI ApaI
AspEI AspHI AvaI AvaII BalI
BanI BanII BbvI BcnI BfaI
BmyI BpmI BsaHI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrI BssKI BstAPI BstF5I BstNI
BstUI Bsu36I Cac8I CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
Eam1105I EarI Eco81I EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MluNI MnlI MscI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NcoI NdeII NlaIII
NlaIV NspI PleI PvuII RsaI
SacI Sau3AI Sau96I ScrFI SfcI
SmaI StuI StyI TaqI TseI
Tsp45I TspRI TthHB8I XmaCI XmaI