Restriction map for
pF1KA0691
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# NO CUT ENZYMES ( 112 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AflIII AgeI AhdI Alw44I AocI
Aor51HI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AvrII
BamHI BbsI BcgI BciVI BclI
BfrI BglI BlnI BlpI Bpu1102I
BpuAI BsaBI BseAI BsiWI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrBI BsrDI BsrGI BssHII Bst1107I
BstBI BstEII BstPI BstZ17I Bsu36I
CelII ClaI CpoI DraI DraIII
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoO65I EcoRV EcoT22I FbaI
FseI HpaI KpnI KspI MamI
MfeI MluI MroI MunI NaeI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PflFI PinAI
PmeI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacII SapI SbfI
SexAI SfiI SfuI SgrAI SmaI
SnaBI SpeI Sse8387I SspBI SspI
SwaI Tth111I XbaI XcmI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AccI 1998 GTCGAC GT^[AC][GT]AC
AccII 111 CGCG CG^CG
AcsI 1981 GAATTC [GA]^AATT[CT]
AlwI 343 GATCC GGATC(4/5)
ApoI 1981 GAATTC [GA]^AATT[CT]
BalI 372 TGGCCA TGG^CCA
BbeI 1273 GGCGCC GGCGC^C
BbrPI 535 CACGTG CAC^GTG
BglII 755 AGATCT A^GATCT
BsaAI 535 CACGTG [CT]AC^GT[GA]
BsaWI 491 ACCGGA [AT]^CCGG[AT]
BsiEI 2000 CGACCG CG[GA][CT]^CG
BsmBI 253 GAGACG CGTCTC(1/5)
BspMI 411 GCAGGT ACCTGC(4/8)
BssSI 2125 CACGAG CACGAG(-5/-1)
BstUI 111 CGCG CG^CG
BstXI 1481 CCAGCCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DrdI 877 GACAGTGAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 372 TGGCCA [CT]^GGCC[GA]
EcoRI 1981 GAATTC G^AATTC
HindIII 302 AAGCTT A^AGCTT
KasI 1273 GGCGCC G^GCGCC
MluNI 372 TGGCCA TGG^CCA
MscI 372 TGGCCA TGG^CCA
MseI 155 TTAA T^TAA
MslI 408 CATGCAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 111 CGCG CG^CG
NarI 1273 GGCGCC GG^CGCC
PaeR7I 691 CTCGAG C^TCGAG
PmaCI 535 CACGTG CAC^GTG
PmlI 535 CACGTG CAC^GTG
PstI 1756 CTGCAG CTGCA^G
SacI 1620 GAGCTC GAGCT^C
SalI 1998 GTCGAC G^TCGAC
ScaI 623 AGTACT AGT^ACT
SfcI 1756 CTGCAG C^T[GA][CT]AG
SfoI 1273 GGCGCC GGC^GCC
StuI 1358 AGGCCT AGG^CCT
Tru9I 155 TTAA T^TAA
Tsp45I 441 GTCAC ^GT[GC]AC
Tsp509I 1982 AATT ^AATT
XhoI 691 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AspHI 1002 GAGCAC G[AT]GC[AT]^C
1620 GAGCTC
AvaI 691 CTCGAG C^[CT]CG[GA]G
1092 CTCGGG
AviII 539 TGCGCA TGC^GCA
560 TGCGCA
BbvCI 1739 CCTCAGC CCTCAGC(-5/-2)
171 GCTGAGG
BfaI 547 CTAG C^TAG
1184 CTAG
BsaI 289 GAGACC GGTCTC(1/5)
550 GAGACC
BseRI 658 GAGGAG GAGGAG(10/8)
2128 GAGGAG
BsgI 1687 CTGCAC GTGCAG(16/14)
451 GTGCAG
BsiHKAI 1002 GAGCAC G[AT]GC[AT]^C
1620 GAGCTC
BsoBI 691 CTCGAG C^[CT]CG[GA]G
1092 CTCGGG
BsrFI 1246 GCCGGT [GA]^CCGG[CT]
1552 GCCGGT
BstAPI 1549 GCAGCCGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1866 GCACCACATGC
BstYI 342 AGATCC [GA]^GATC[CT]
755 AGATCT
Cfr10I 1246 GCCGGT [GA]^CCGG[CT]
1552 GCCGGT
FspI 539 TGCGCA TGC^GCA
560 TGCGCA
MaeI 547 CTAG C^TAG
1184 CTAG
MflI 342 AGATCC [GA]^GATC[CT]
755 AGATCT
NcoI 1634 CCATGG C^CATGG
1847 CCATGG
PflMI 1116 CCAGGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1356 CCAGGCCTTGG
PvuII 950 CAGCTG CAG^CTG
1759 CAGCTG
SfaNI 1540 GATGC GCATC(5/9)
1953 GATGC
SphI 542 GCATGC GCATG^C
563 GCATGC
TfiI 1506 GAATC G^A[AT]TC
2090 GATTC
Van91I 1116 CCAGGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1356 CCAGGCCTTGG
XhoII 342 AGATCC [GA]^GATC[CT]
755 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 79 enzymes) #
#########################################
AciI AcyI AfaI AluI AlwNI
ApaI AvaII BanI BanII BbvI
BcnI BmyI BpmI BsaHI BsaJI
BsiYI BslI BsmAI BsmFI Bsp1286I
BsrI BssKI BstF5I BstNI Cac8I
CfoI Cfr13I DdeI DpnII DraII
DsaI EarI EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HincII HindII HinfI HpaII
HphI ItaI Ksp632I MaeII MaeIII
MboI MboII MnlI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV NspI PleI PpuMI RsaI
Sau3AI Sau96I ScrFI SmlI StyI
TaqI TseI TspRI TthHB8I