Restriction map for
pF1KA0651
##################################
# NO CUT ENZYMES ( 103 enzymes) #
##################################
AatII AccI AclI AcsI AflII
AflIII AgeI AhdI Aor51HI ApoI
AscI AseI AsnI Asp700I AspEI
AspI AvrII BalI BbeI BbrPI
BcgI BfrI BlnI BlpI Bpu1102I
BsaAI BsaBI BsaI BsiEI Bsp1407I
BspDI BspLU11I BsrGI BssHII Bst1107I
BstEII BstPI BstZ17I CelII ClaI
CpoI DraI DrdI EagI Eam1105I
EclXI Eco47III Eco52I EcoNI EcoO65I
EcoRI EcoRV EcoT22I FseI HincII
HindII HindIII HpaI KasI KspI
MaeII MamI MfeI MluI MluNI
MscI MunI NarI NcoI NdeI
NheI NotI NruI NsiI PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RsrII SacII SalI SbfI ScaI
SexAI SfoI SgrAI SnaBI SpeI
Sse8387I SspBI SspI SwaI Tth111I
XbaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
Acc65I 280 GGTACC G^GTACC
AccII 3211 CGCG CG^CG
AccIII 1379 TCCGGA T^CCGGA
AcyI 1184 GACGCC G[GA]^CG[CT]C
Alw44I 1933 GTGCAC G^TGCAC
ApaLI 1933 GTGCAC G^TGCAC
Asp718I 280 GGTACC G^GTACC
AviII 1991 TGCGCA TGC^GCA
BbsI 1725 GAAGAC GAAGAC(2/6)
BclI 1901 TGATCA T^GATCA
BglII 1613 AGATCT A^GATCT
BpuAI 1725 GAAGAC GAAGAC(2/6)
BsaHI 1184 GACGCC G[GA]^CG[CT]C
BseAI 1379 TCCGGA T^CCGGA
BsgI 1024 CTGCAC GTGCAG(16/14)
BsiWI 1863 CGTACG C^GTACG
BsmBI 3199 GAGACG CGTCTC(1/5)
BspEI 1379 TCCGGA T^CCGGA
BspHI 1049 TCATGA T^CATGA
BsrFI 2744 GCCGGC [GA]^CCGG[CT]
BstBI 614 TTCGAA TT^CGAA
BstUI 3211 CGCG CG^CG
Cfr10I 2744 GCCGGC [GA]^CCGG[CT]
DraIII 1027 CACCATGTG CAC[ACGT][ACGT][ACGT]^GTG
FbaI 1901 TGATCA T^GATCA
FspI 1991 TGCGCA TGC^GCA
HgaI 1184 GACGC GACGC(5/10)
Hin1I 1184 GACGCC G[GA]^CG[CT]C
KpnI 280 GGTACC GGTAC^C
MroI 1379 TCCGGA T^CCGGA
MvnI 3211 CGCG CG^CG
NaeI 2744 GCCGGC GCC^GGC
NgoMIV 2744 GCCGGC G^CCGGC
NspV 614 TTCGAA TT^CGAA
RcaI 1049 TCATGA T^CATGA
SacI 1135 GAGCTC GAGCT^C
SfuI 614 TTCGAA TT^CGAA
SphI 149 GCATGC GCATG^C
StuI 1322 AGGCCT AGG^CCT
###################################
# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AocI 243 CCTCAGG CC^T[ACGT]AGG
2584 CCTGAGG
BanI 280 GGTACC G^G[CT][GA]CC
401 GGCACC
BbvCI 1161 CCTCAGC CCTCAGC(-5/-2)
2893 GCTGAGG
BciVI 204 GGATAC GTATCC(6/5)
220 GTATCC
BsmI 3179 GAATGC GAATGC(1/-1)
1491 GCATTC
BstAPI 1983 GCAGAGCGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2947 GCAGGCGATGC
BstXI 1529 CCACAGCACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2684 CCACGGAACTGG
Bsu36I 243 CCTCAGG CC^T[ACGT]AGG
2584 CCTGAGG
Eco81I 243 CCTCAGG CC^T[ACGT]AGG
2584 CCTGAGG
MseI 1327 TTAA T^TAA
2060 TTAA
PpuMI 1263 AGGTCCT [GA]G^G[AT]CC[CT]
1520 AGGACCT
SapI 1081 GAAGAGC GCTCTTC(1/4)
3082 GAAGAGC
SfiI 1663 GGCCCCTTTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
2898 GGCCCCCTGGGCC
Tru9I 1327 TTAA T^TAA
2060 TTAA
Tsp509I 1373 AATT ^AATT
2058 AATT
XcmI 135 CCACTCGGCCACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2255 CCATCCGTGAGGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 100 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
ApaI AspHI AvaI AvaII BamHI
BanII BbvI BcnI BfaI BglI
BmyI BpmI BsaJI BsaWI BseRI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrBI BsrDI
BsrI BssKI BssSI BstF5I BstNI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeIII MboI MboII
MflI MnlI MslI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV NspI PflMI PleI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmaI SmlI StyI
TaqI TfiI TseI Tsp45I TspRI
TthHB8I Van91I XhoII XmaCI XmaI