Restriction map for
pF1KB8829
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# NO CUT ENZYMES ( 163 enzymes) #
##################################
AatII Acc65I AccI AccII AciI
AclI AcyI AflII AgeI AhdI
Alw44I AlwI AocI Aor51HI ApaI
ApaLI AscI Asp718I AspEI AspHI
AspI AvaI AviII AvrII BalI
BamHI BanI BanII BbeI BbrPI
BbvCI BbvI BcgI BciVI BclI
BcnI BfrI BglI BlnI BlpI
BmyI Bpu1102I BsaAI BsaHI BsaI
BseRI BsiEI BsiHKAI BsiWI BsmBI
BsoBI Bsp1286I Bsp1407I BspDI BspMI
BsrBI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstEII BstPI BstUI
BstXI BstZ17I Bsu36I CelII CfoI
Cfr10I ClaI CpoI DraI DraII
DraIII DrdI DsaI EaeI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoO109I EcoO65I EcoRV EcoT22I FauI
FbaI Fnu4HI FseI FspI HaeII
HgaI HhaI Hin1I HinP1I HincII
HindII HpaI ItaI KasI KpnI
KspI MluI MluNI MscI MspA1I
MvnI NaeI NarI NciI NcoI
NgoMIV NheI NotI NruI NsiI
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
Psp1406I PstI PvuI PvuII RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfaNI SfcI SfiI
SfoI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
TseI Tth111I Van91I XbaI XcmI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AccIII 63 TCCGGA T^CCGGA
AflIII 121 ACATGT A^C[GA][CT]GT
AlwNI 1269 CAGTTACTG CAG[ACGT][ACGT][ACGT]^CTG
AseI 1303 ATTAAT AT^TAAT
AsnI 1303 ATTAAT AT^TAAT
AvaII 385 GGACC G^G[AT]CC
BfaI 293 CTAG C^TAG
BglII 1009 AGATCT A^GATCT
BsaBI 143 GATATTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 63 TCCGGA [AT]^CCGG[AT]
BseAI 63 TCCGGA T^CCGGA
BsgI 23 GTGCAG GTGCAG(16/14)
BsmFI 677 GTCCC GGGAC(10/14)
BspEI 63 TCCGGA T^CCGGA
BspHI 948 TCATGA T^CATGA
BspLU11I 121 ACATGT A^CATGT
BstF5I 149 CATCC GGATG(2/0)
BstYI 1009 AGATCT [GA]^GATC[CT]
Cfr13I 385 GGACC G^G[ACGT]CC
DdeI 427 CTCAG C^T[ACGT]AG
DpnII 1010 GATC ^GATC
EarI 89 CTCTTC CTCTTC(1/4)
EcoNI 152 CCTTTAATAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 1208 GAATTC G^AATTC
EcoT14I 416 CCTTGG C^C[AT][AT]GG
FokI 149 CATCC GGATG(9/13)
HapII 64 CCGG C^CGG
HindIII 768 AAGCTT A^AGCTT
HpaII 64 CCGG C^CGG
Ksp632I 89 CTCTTC CTCTTC(1/4)
MaeI 293 CTAG C^TAG
MaeII 237 ACGT A^CGT
MamI 143 GATATTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MboI 1010 GATC ^GATC
MfeI 672 CAATTG C^AATTG
MflI 1009 AGATCT [GA]^GATC[CT]
MroI 63 TCCGGA T^CCGGA
MspI 64 CCGG C^CGG
MunI 672 CAATTG C^AATTG
MwoI 761 GCCTTATAAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NdeI 557 CATATG CA^TATG
NdeII 1010 GATC ^GATC
NlaIV 60 GGTTCC GG[ACGT]^[ACGT]CC
PshBI 1303 ATTAAT AT^TAAT
RcaI 948 TCATGA T^CATGA
Sau3AI 1010 GATC ^GATC
Sau96I 385 GGACC G^G[ACGT]CC
SmlI 249 CTTGAG C^T[CT][GA]AG
StyI 416 CCTTGG C^C[AT][AT]GG
TfiI 524 GAATC G^A[AT]TC
Tsp45I 875 GTCAC ^GT[GC]AC
XhoII 1009 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AcsI 205 AAATTT [GA]^AATT[CT]
1208 GAATTC
AluI 769 AGCT AG^CT
1102 AGCT
ApoI 205 AAATTT [GA]^AATT[CT]
1208 GAATTC
BbsI 67 GAAGAC GAAGAC(2/6)
776 GAAGAC
BpuAI 67 GAAGAC GAAGAC(2/6)
776 GAAGAC
BsaJI 56 CCCAGG C^C[ACGT][ACGT]GG
416 CCTTGG
BsmAI 9 GAGAC GTCTC(1/5)
425 GTCTC
BsmI 788 GAATGC GAATGC(1/-1)
350 GCATTC
BssKI 57 CCAGG ^CC[ACGT]GG
94 CCTGG
BstNI 57 CCAGG CC^[AT]GG
94 CCTGG
Cac8I 346 GCAAGC GC[ACGT]^[ACGT]GC
902 GCAAGC
EcoRII 57 CCAGG ^CC[AT]GG
94 CCTGG
HaeIII 284 GGCC GG^CC
806 GGCC
HphI 1051 GGTGA GGTGA(8/7)
53 TCACC
MseI 155 TTAA T^TAA
1304 TTAA
MvaI 57 CCAGG CC^[AT]GG
94 CCTGG
NspI 71 ACATGC [GA]CATG^[CT]
121 ACATGT
ScrFI 57 CCAGG CC^[ACGT]GG
94 CCTGG
StuI 283 AGGCCT AGG^CCT
805 AGGCCT
Tru9I 155 TTAA T^TAA
1304 TTAA
#########################################
# 3 OR MORE CUT ENZYMES ( 23 enzymes) #
#########################################
AfaI Asp700I BpmI BsiYI BslI
BsrDI BsrI BstBI HinfI MaeIII
MboII MnlI MslI NlaIII NspV
PleI RsaI SfuI TaqI Tsp509I
TspRI TthHB8I XmnI