Restriction map for
pF1KA0619
##################################
# NO CUT ENZYMES ( 89 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AgeI AhdI Alw44I ApaI ApaLI
AscI Asp718I AspEI AspI AviII
AvrII BalI BamHI BfrI BlnI
BlpI Bpu1102I BseAI BsiEI BsiWI
BsmBI BsmI BspDI BspEI BspMI
BsrBI BssHII BssSI BstAPI BstEII
BstPI CelII ClaI CpoI DraII
DraIII EagI Eam1105I EclXI Eco52I
EcoNI EcoO109I EcoO65I EcoRV EcoT22I
FseI FspI HgaI KpnI KspI
MluI MluNI MroI MscI NheI
NotI NruI NsiI PacI PaeR7I
PflFI PinAI PmeI PpuMI PshAI
Psp1406I PvuI RsrII SacII SbfI
ScaI SexAI SfiI SgrAI SmaI
SnaBI SphI Sse8387I StuI SwaI
Tth111I XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 54 enzymes) #
###################################
AcyI 34 GGCGCC G[GA]^CG[CT]C
AocI 775 CCTGAGG CC^T[ACGT]AGG
Aor51HI 2405 AGCGCT AGC^GCT
AseI 1921 ATTAAT AT^TAAT
AsnI 1921 ATTAAT AT^TAAT
Asp700I 589 GAAGTTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 40 CCCGAG C^[CT]CG[GA]G
BanI 34 GGCGCC G^G[CT][GA]CC
BbeI 34 GGCGCC GGCGC^C
BbrPI 702 CACGTG CAC^GTG
BbsI 268 GAAGAC GAAGAC(2/6)
BbvCI 3424 GCTGAGG CCTCAGC(-5/-2)
BciVI 870 GGATAC GTATCC(6/5)
BpuAI 268 GAAGAC GAAGAC(2/6)
BsaAI 702 CACGTG [CT]AC^GT[GA]
BsaBI 484 GATAGGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 34 GGCGCC G[GA]^CG[CT]C
BsaWI 759 ACCGGA [AT]^CCGG[AT]
BsgI 316 GTGCAG GTGCAG(16/14)
BsoBI 40 CCCGAG C^[CT]CG[GA]G
Bsp1407I 494 TGTACA T^GTACA
BspLU11I 3848 ACATGT A^CATGT
BsrFI 5 GCCGGC [GA]^CCGG[CT]
BsrGI 494 TGTACA T^GTACA
Bst1107I 1347 GTATAC GTA^TAC
BstXI 119 CCATCAACGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 1347 GTATAC GTA^TAC
Bsu36I 775 CCTGAGG CC^T[ACGT]AGG
Cfr10I 5 GCCGGC [GA]^CCGG[CT]
DrdI 4127 GACGGCCAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 4129 CGGCCA [CT]^GGCC[GA]
Eco47III 2405 AGCGCT AGC^GCT
Eco81I 775 CCTGAGG CC^T[ACGT]AGG
EcoRI 930 GAATTC G^AATTC
FauI 12 CCCGC CCCGC(4/6)
Hin1I 34 GGCGCC G[GA]^CG[CT]C
KasI 34 GGCGCC G^GCGCC
MamI 484 GATAGGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI 5 GCCGGC GCC^GGC
NarI 34 GGCGCC GG^CGCC
NgoMIV 5 GCCGGC G^CCGGC
PflMI 2496 CCATCTCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 702 CACGTG CAC^GTG
PmlI 702 CACGTG CAC^GTG
PshBI 1921 ATTAAT AT^TAAT
SalI 4136 GTCGAC G^TCGAC
SapI 4003 GAAGAGC GCTCTTC(1/4)
SfoI 34 GGCGCC GGC^GCC
SpeI 2935 ACTAGT A^CTAGT
SspBI 494 TGTACA T^GTACA
SspI 2804 AATATT AAT^ATT
Van91I 2496 CCATCTCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 1878 TCTAGA T^CTAGA
XmnI 589 GAAGTTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AccII 48 CGCG CG^CG
69 CGCG
AflIII 703 ACGTGT A^C[GA][CT]GT
3848 ACATGT
AspHI 2329 GAGCTC G[AT]GC[AT]^C
2581 GAGCTC
AvaII 159 GGTCC G^G[AT]CC
3412 GGACC
BanII 2329 GAGCTC G[GA]GC[CT]^C
2581 GAGCTC
BglI 2128 GCCACAAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
3837 GCCCCTGTGGC
BglII 1800 AGATCT A^GATCT
2159 AGATCT
BmyI 2329 GAGCTC G[AGT]GC[ACT]^C
2581 GAGCTC
BsiHKAI 2329 GAGCTC G[AT]GC[AT]^C
2581 GAGCTC
Bsp1286I 2329 GAGCTC G[AGT]GC[ACT]^C
2581 GAGCTC
BsrDI 3473 CATTGC GCAATG(2/0)
1370 GCAATG
BstBI 2543 TTCGAA TT^CGAA
3302 TTCGAA
BstUI 48 CGCG CG^CG
69 CGCG
BstYI 1800 AGATCT [GA]^GATC[CT]
2159 AGATCT
DraI 1962 TTTAAA TTT^AAA
2235 TTTAAA
DsaI 620 CCATGG C^C[GA][CT]GG
1905 CCATGG
HindIII 361 AAGCTT A^AGCTT
1202 AAGCTT
HpaI 1597 GTTAAC GTT^AAC
2449 GTTAAC
MfeI 2789 CAATTG C^AATTG
2900 CAATTG
MflI 1800 AGATCT [GA]^GATC[CT]
2159 AGATCT
MunI 2789 CAATTG C^AATTG
2900 CAATTG
MvnI 48 CGCG CG^CG
69 CGCG
NcoI 620 CCATGG C^CATGG
1905 CCATGG
NdeI 3199 CATATG CA^TATG
3910 CATATG
NspV 2543 TTCGAA TT^CGAA
3302 TTCGAA
PstI 1730 CTGCAG CTGCA^G
3313 CTGCAG
SacI 2329 GAGCTC GAGCT^C
2581 GAGCTC
SfuI 2543 TTCGAA TT^CGAA
3302 TTCGAA
SmlI 2911 CTTGAG C^T[CT][GA]AG
3095 CTCAAG
XhoII 1800 AGATCT [GA]^GATC[CT]
2159 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 85 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI AlwNI ApoI BbvI BcgI
BclI BcnI BfaI BpmI BsaI
BsaJI BseRI BsiYI BslI BsmAI
BsmFI BspHI BsrI BssKI BstF5I
BstNI Cac8I CfoI Cfr13I DdeI
DpnII EarI EcoRII EcoT14I FbaI
Fnu4HI FokI HaeII HaeIII HapII
HhaI HinP1I HincII HindII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MnlI
MseI MslI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
NspI PleI PvuII RcaI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XcmI