Restriction map for
pF1KA0583
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# NO CUT ENZYMES ( 53 enzymes) #
##################################
AatII AclI AgeI AscI AseI
AsnI AvrII BamHI BciVI BclI
BlnI BsaBI BsiWI BspDI BspLU11I
BssHII Bst1107I BstZ17I ClaI CpoI
DraI DrdI EcoT22I FbaI FseI
HpaI MamI MfeI MunI NdeI
NheI NotI NruI NsiI PacI
PinAI PmeI PshAI PshBI Psp1406I
PvuI RsrII SapI SbfI ScaI
SfiI SgrAI SnaBI SphI Sse8387I
SspI SwaI XbaI
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# ONE CUT ENZYMES ( 37 enzymes) #
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Acc65I 4342 GGTACC G^GTACC
AccI 5365 GTCGAC GT^[AC][GT]AC
AccIII 1619 TCCGGA T^CCGGA
AcsI 4978 GAATTC [GA]^AATT[CT]
AflII 2004 CTTAAG C^TTAAG
AflIII 4500 ACGCGT A^C[GA][CT]GT
Aor51HI 102 AGCGCT AGC^GCT
ApoI 4978 GAATTC [GA]^AATT[CT]
Asp718I 4342 GGTACC G^GTACC
BfrI 2004 CTTAAG C^TTAAG
BglI 2071 GCCATCAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 5696 AGATCT A^GATCT
BsaWI 1619 TCCGGA [AT]^CCGG[AT]
BseAI 1619 TCCGGA T^CCGGA
Bsp1407I 1283 TGTACA T^GTACA
BspEI 1619 TCCGGA T^CCGGA
BspHI 1304 TCATGA T^CATGA
BsrGI 1283 TGTACA T^GTACA
BstBI 1493 TTCGAA TT^CGAA
DraIII 1625 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 3358 CGGCCG C^GGCCG
EclXI 3358 CGGCCG C^GGCCG
Eco47III 102 AGCGCT AGC^GCT
Eco52I 3358 CGGCCG C^GGCCG
EcoRI 4978 GAATTC G^AATTC
EcoRV 3842 GATATC GAT^ATC
KpnI 4342 GGTACC GGTAC^C
MluI 4500 ACGCGT A^CGCGT
MroI 1619 TCCGGA T^CCGGA
NspV 1493 TTCGAA TT^CGAA
RcaI 1304 TCATGA T^CATGA
SalI 5365 GTCGAC G^TCGAC
SexAI 1319 ACCAGGT A^CC[AT]GGT
SfuI 1493 TTCGAA TT^CGAA
SpeI 3133 ACTAGT A^CTAGT
SspBI 1283 TGTACA T^GTACA
Tsp509I 4979 AATT ^AATT
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# TWO CUT ENZYMES ( 32 enzymes) #
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AhdI 2308 GACAACAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3928 GACACCCTGTC
Alw44I 4291 GTGCAC G^TGCAC
4540 GTGCAC
ApaI 2769 GGGCCC GGGCC^C
4796 GGGCCC
ApaLI 4291 GTGCAC G^TGCAC
4540 GTGCAC
Asp700I 1486 GAAATCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2379 GAAGGTATTC
AspEI 2308 GACAACAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3928 GACACCCTGTC
AviII 1685 TGCGCA TGC^GCA
3596 TGCGCA
BbeI 148 GGCGCC GGCGC^C
387 GGCGCC
BbrPI 4048 CACGTG CAC^GTG
4288 CACGTG
BsaI 2758 GAGACC GGTCTC(1/5)
5555 GAGACC
BsiEI 3358 CGGCCG CG[GA][CT]^CG
4806 CGACCG
BsmBI 4119 CGTCTC CGTCTC(1/5)
1876 GAGACG
BstEII 838 GGTGACC G^GT[ACGT]ACC
4128 GGTGACC
BstPI 838 GGTGACC G^GT[ACGT]ACC
4128 GGTGACC
Eam1105I 2308 GACAACAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3928 GACACCCTGTC
EarI 639 GAAGAG CTCTTC(1/4)
963 GAAGAG
EcoO65I 838 GGTGACC G^GT[ACGT]ACC
4128 GGTGACC
FspI 1685 TGCGCA TGC^GCA
3596 TGCGCA
HincII 3988 GTCAAC GT[CT]^[GA]AC
5365 GTCGAC
HindII 3988 GTCAAC GT[CT]^[GA]AC
5365 GTCGAC
HindIII 3364 AAGCTT A^AGCTT
5623 AAGCTT
KasI 148 GGCGCC G^GCGCC
387 GGCGCC
Ksp632I 639 GAAGAG CTCTTC(1/4)
963 GAAGAG
KspI 95 CCGCGG CCGC^GG
3847 CCGCGG
NarI 148 GGCGCC GG^CGCC
387 GGCGCC
PaeR7I 88 CTCGAG C^TCGAG
4162 CTCGAG
PmaCI 4048 CACGTG CAC^GTG
4288 CACGTG
PmlI 4048 CACGTG CAC^GTG
4288 CACGTG
SacII 95 CCGCGG CCGC^GG
3847 CCGCGG
SfoI 148 GGCGCC GGC^GCC
387 GGCGCC
XhoI 88 CTCGAG C^TCGAG
4162 CTCGAG
XmnI 1486 GAAATCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2379 GAAGGTATTC
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# 3 OR MORE CUT ENZYMES ( 136 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI AocI AspHI AspI
AvaI AvaII BalI BanI BanII
BbsI BbvCI BbvI BcgI BcnI
BfaI BlpI BmyI BpmI Bpu1102I
BpuAI BsaAI BsaHI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI BsoBI Bsp1286I BspMI
BsrBI BsrDI BsrFI BsrI BssKI
BssSI BstAPI BstF5I BstNI BstUI
BstXI BstYI Bsu36I Cac8I CelII
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI Eco81I EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NaeI NciI
NcoI NdeII NgoMIV NlaIII NlaIV
NspI PflFI PflMI PleI PpuMI
PstI PvuII RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI SmaI
SmlI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I TspRI Tth111I
TthHB8I Van91I XcmI XhoII XmaCI
XmaI