Restriction map for
pF1KA0575
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# NO CUT ENZYMES ( 84 enzymes) #
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AatII Acc65I AccIII AclI AcsI
AflII AhdI ApaI ApoI AscI
AseI AsnI Asp718I AspEI AspI
AviII AvrII BamHI BbvCI BcgI
BciVI BclI BfrI BglI BglII
BlnI BseAI BsiWI BsmBI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrGI BssHII Bst1107I BstBI BstZ17I
ClaI CpoI DrdI Eam1105I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HindIII HpaI KpnI MfeI MluI
MroI MunI NcoI NdeI NotI
NruI NsiI NspV PacI PflFI
PmeI PshBI Psp1406I PvuI RcaI
RsrII SapI ScaI SexAI SfiI
SfuI SgrAI SnaBI SpeI SspBI
SwaI Tth111I XbaI XcmI
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# ONE CUT ENZYMES ( 35 enzymes) #
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AgeI 2201 ACCGGT A^CCGGT
AocI 603 CCTCAGG CC^T[ACGT]AGG
Aor51HI 411 AGCGCT AGC^GCT
Asp700I 2199 GAACCGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI 1189 TGGCCA TGG^CCA
BbeI 693 GGCGCC GGCGC^C
BsaBI 1131 GATCAATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 30 GAGACC GGTCTC(1/5)
BsrDI 1684 GCAATG GCAATG(2/0)
BstAPI 2022 GCACATCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 636 GGTCACC G^GT[ACGT]ACC
BstPI 636 GGTCACC G^GT[ACGT]ACC
Bsu36I 603 CCTCAGG CC^T[ACGT]AGG
DraI 2577 TTTAAA TTT^AAA
DraIII 2497 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 411 AGCGCT AGC^GCT
Eco81I 603 CCTCAGG CC^T[ACGT]AGG
EcoO65I 636 GGTCACC G^GT[ACGT]ACC
KasI 693 GGCGCC G^GCGCC
KspI 2357 CCGCGG CCGC^GG
MamI 1131 GATCAATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 1189 TGGCCA TGG^CCA
MscI 1189 TGGCCA TGG^CCA
NarI 693 GGCGCC GG^CGCC
NheI 2647 GCTAGC G^CTAGC
PinAI 2201 ACCGGT A^CCGGT
PshAI 2727 GACGGTCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacII 2357 CCGCGG CCGC^GG
SalI 2512 GTCGAC G^TCGAC
SbfI 717 CCTGCAGG CCTGCA^GG
SfoI 693 GGCGCC GGC^GCC
Sse8387I 717 CCTGCAGG CCTGCA^GG
SspI 1751 AATATT AAT^ATT
Tsp509I 1740 AATT ^AATT
XmnI 2199 GAACCGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 27 enzymes) #
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AccI 2221 GTAGAC GT^[AC][GT]AC
2512 GTCGAC
AflIII 2009 ACGTGT A^C[GA][CT]GT
2402 ACACGT
Alw44I 2143 GTGCAC G^TGCAC
2239 GTGCAC
AlwI 1500 GATCC GGATC(4/5)
2533 GGATC
AlwNI 181 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG
2425 CAGCTCCTG
ApaLI 2143 GTGCAC G^TGCAC
2239 GTGCAC
BsmAI 30 GAGAC GTCTC(1/5)
1359 GAGAC
BssSI 784 CTCGTG CACGAG(-5/-1)
1591 CTCGTG
BstXI 477 CCAGCCCCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
953 CCAGCCACATGG
BstYI 1499 AGATCC [GA]^GATC[CT]
2533 GGATCT
EagI 1647 CGGCCG C^GGCCG
2809 CGGCCG
EarI 2464 CTCTTC CTCTTC(1/4)
330 GAAGAG
EclXI 1647 CGGCCG C^GGCCG
2809 CGGCCG
Eco52I 1647 CGGCCG C^GGCCG
2809 CGGCCG
HincII 1960 GTCAAC GT[CT]^[GA]AC
2512 GTCGAC
HindII 1960 GTCAAC GT[CT]^[GA]AC
2512 GTCGAC
Ksp632I 2464 CTCTTC CTCTTC(1/4)
330 GAAGAG
MflI 1499 AGATCC [GA]^GATC[CT]
2533 GGATCT
MseI 1670 TTAA T^TAA
2578 TTAA
NspI 462 GCATGC [GA]CATG^[CT]
1577 GCATGC
PaeR7I 1858 CTCGAG C^TCGAG
2746 CTCGAG
SmlI 1858 CTCGAG C^T[CT][GA]AG
2746 CTCGAG
SphI 462 GCATGC GCATG^C
1577 GCATGC
TfiI 1153 GAATC G^A[AT]TC
1886 GAATC
Tru9I 1670 TTAA T^TAA
2578 TTAA
XhoI 1858 CTCGAG C^TCGAG
2746 CTCGAG
XhoII 1499 AGATCC [GA]^GATC[CT]
2533 GGATCT
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# 3 OR MORE CUT ENZYMES ( 112 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AspHI AvaI AvaII BanI BanII
BbrPI BbsI BbvI BcnI BfaI
BlpI BmyI BpmI Bpu1102I BpuAI
BsaAI BsaHI BsaJI BsaWI BseRI
BsgI BsiEI BsiHKAI BsiYI BslI
BsmFI BsoBI Bsp1286I BspMI BsrBI
BsrFI BsrI BssKI BstF5I BstNI
BstUI Cac8I CelII CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI MaeI
MaeII MaeIII MboI MboII MnlI
MslI MspA1I MspI MvaI MvnI
MwoI NaeI NciI NdeII NgoMIV
NlaIII NlaIV PflMI PleI PmaCI
PmlI PpuMI PstI PvuII RsaI
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI SmaI StuI StyI TaqI
TseI Tsp45I TspRI TthHB8I Van91I
XmaCI XmaI