Restriction map for
pF1KA0555
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# NO CUT ENZYMES ( 108 enzymes) #
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AatII Acc65I AccI AccIII AclI
AhdI Alw44I AlwNI ApaI ApaLI
AscI AseI AsnI Asp718I AspEI
AspI AviII AvrII BanI BbeI
BbrPI BcgI BciVI BclI BglI
BlnI BlpI Bpu1102I BsaBI BseAI
BsgI BsiEI BsiWI BspDI BspEI
BspHI BspLU11I BssHII Bst1107I BstEII
BstPI BstXI BstZ17I CelII ClaI
CpoI DraI DrdI EagI Eam1105I
EclXI Eco52I EcoO65I EcoT22I FbaI
FseI FspI HincII HindII HpaI
KasI KpnI KspI MamI MfeI
MluI MroI MslI MunI NaeI
NarI NgoMIV NheI NotI NruI
NsiI NspI PacI PaeR7I PflFI
PflMI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacII SalI SbfI ScaI SexAI
SfiI SfoI SgrAI SnaBI SpeI
SphI Sse8387I SspI SwaI Tth111I
Van91I XbaI XhoI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AccII 364 CGCG CG^CG
AcyI 1663 GACGCC G[GA]^CG[CT]C
AflII 448 CTTAAG C^TTAAG
AflIII 942 ACGTGT A^C[GA][CT]GT
AgeI 1640 ACCGGT A^CCGGT
AocI 75 CCTCAGG CC^T[ACGT]AGG
Aor51HI 393 AGCGCT AGC^GCT
BalI 1889 TGGCCA TGG^CCA
BamHI 1934 GGATCC G^GATCC
BfrI 448 CTTAAG C^TTAAG
BglII 1784 AGATCT A^GATCT
BsaAI 317 TACGTG [CT]AC^GT[GA]
BsaHI 1663 GACGCC G[GA]^CG[CT]C
BsaWI 1640 ACCGGT [AT]^CCGG[AT]
BsmBI 1722 GAGACG CGTCTC(1/5)
BsmI 873 GAATGC GAATGC(1/-1)
BsrBI 607 GAGCGG CCGCTC(-3/-3)
BsrDI 963 GCAATG GCAATG(2/0)
BsrFI 1640 ACCGGT [GA]^CCGG[CT]
BsrI 678 ACTGG ACTGG(1/-1)
BssSI 286 CACGAG CACGAG(-5/-1)
BstAPI 195 GCACACGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 1694 TTCGAA TT^CGAA
BstUI 364 CGCG CG^CG
Bsu36I 75 CCTCAGG CC^T[ACGT]AGG
Cfr10I 1640 ACCGGT [GA]^CCGG[CT]
DraIII 196 CACACGGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 1343 CCATGG C^C[GA][CT]GG
Eco47III 393 AGCGCT AGC^GCT
Eco81I 75 CCTCAGG CC^T[ACGT]AGG
EcoRI 2285 GAATTC G^AATTC
EcoRV 1846 GATATC GAT^ATC
Hin1I 1663 GACGCC G[GA]^CG[CT]C
HindIII 136 AAGCTT A^AGCTT
MluNI 1889 TGGCCA TGG^CCA
MscI 1889 TGGCCA TGG^CCA
MspA1I 1409 CAGCTG C[AC]G^C[GT]G
MvnI 364 CGCG CG^CG
NcoI 1343 CCATGG C^CATGG
NdeI 1472 CATATG CA^TATG
NspV 1694 TTCGAA TT^CGAA
PinAI 1640 ACCGGT A^CCGGT
PpuMI 545 GGGACCT [GA]G^G[AT]CC[CT]
PvuII 1409 CAGCTG CAG^CTG
SapI 2217 GAAGAGC GCTCTTC(1/4)
SfuI 1694 TTCGAA TT^CGAA
SmaI 410 CCCGGG CCC^GGG
StuI 1282 AGGCCT AGG^CCT
XcmI 485 CCAAGAAGCAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 410 CCCGGG C^CCGGG
XmaI 410 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 20 enzymes) #
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Asp700I 1259 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2344 GAAAAGTTTC
AvaI 34 CCCGAG C^[CT]CG[GA]G
410 CCCGGG
BanII 1213 GAGCTC G[GA]GC[CT]^C
2260 GAGCTC
BsaI 682 GAGACC GGTCTC(1/5)
1670 GAGACC
BsoBI 34 CCCGAG C^[CT]CG[GA]G
410 CCCGGG
Bsp1407I 699 TGTACA T^GTACA
2105 TGTACA
BspMI 492 GCAGGT ACCTGC(4/8)
805 GCAGGT
BsrGI 699 TGTACA T^GTACA
2105 TGTACA
DraII 545 GGGACCT [GA]G^G[ACGT]CC[CT]
1457 AGGCCCT
EaeI 482 CGGCCA [CT]^GGCC[GA]
1889 TGGCCA
EcoNI 666 CCTGGAAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1479 CCTCCTACAGG
EcoO109I 545 GGGACCT [GA]G^G[ACGT]CC[CT]
1457 AGGCCCT
FauI 609 GCGGG CCCGC(4/6)
948 GCGGG
HaeII 393 AGCGCT [GA]GCGC^[CT]
443 AGCGCC
HgaI 1510 GACGC GACGC(5/10)
1663 GACGC
MaeII 318 ACGT A^CGT
942 ACGT
PstI 253 CTGCAG CTGCA^G
825 CTGCAG
SacI 1213 GAGCTC GAGCT^C
2260 GAGCTC
SspBI 699 TGTACA T^GTACA
2105 TGTACA
XmnI 1259 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2344 GAAAAGTTTC
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# 3 OR MORE CUT ENZYMES ( 79 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AspHI AvaII BbsI BbvCI
BbvI BcnI BfaI BmyI BpmI
BpuAI BsaJI BseRI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BssKI
BstF5I BstNI BstYI Cac8I CfoI
Cfr13I DdeI DpnII EarI EcoRII
EcoT14I Fnu4HI FokI HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeIII MboI
MboII MflI MnlI MseI MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV PleI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII