Restriction map for
pF1KA0553
##################################
# NO CUT ENZYMES ( 91 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AflIII AgeI AhdI AscI Asp700I
Asp718I AspEI AspI AviII AvrII
BbrPI BcgI BciVI BfrI BlnI
BsaAI BsaBI BsaWI BseAI BsiEI
BsiWI BsmBI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrGI BssHII Bst1107I
BstBI BstEII BstPI BstZ17I ClaI
CpoI DrdI EagI Eam1105I EclXI
Eco52I EcoO65I EcoRV EcoT22I FseI
FspI HindIII HpaI KpnI KspI
MamI MluI MroI NdeI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
Psp1406I PvuI RcaI RsrII SacII
SalI SbfI ScaI SexAI SfiI
SfuI SgrAI SnaBI SpeI Sse8387I
SspBI SwaI Tth111I XbaI XhoI
XmnI
###################################
# ONE CUT ENZYMES ( 35 enzymes) #
###################################
AccI 2417 GTAGAC GT^[AC][GT]AC
AccII 3227 CGCG CG^CG
Alw44I 389 GTGCAC G^TGCAC
ApaLI 389 GTGCAC G^TGCAC
AseI 1431 ATTAAT AT^TAAT
AsnI 1431 ATTAAT AT^TAAT
AvaI 2633 CCCGGG C^[CT]CG[GA]G
BamHI 3627 GGATCC G^GATCC
BglI 679 GCCAAAAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 3745 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 3745 GCTCAGC GC^T[ACGT]AGC
BsaI 1276 GAGACC GGTCTC(1/5)
BsoBI 2633 CCCGGG C^[CT]CG[GA]G
BsrDI 647 CATTGC GCAATG(2/0)
BssSI 2800 CACGAG CACGAG(-5/-1)
BstUI 3227 CGCG CG^CG
CelII 3745 GCTCAGC GC^T[ACGT]AGC
DraI 1492 TTTAAA TTT^AAA
DraIII 3421 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoNI 1663 CCTGCTTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 520 GAATTC G^AATTC
MfeI 137 CAATTG C^AATTG
MunI 137 CAATTG C^AATTG
MvnI 3227 CGCG CG^CG
NaeI 2336 GCCGGC GCC^GGC
NgoMIV 2336 GCCGGC G^CCGGC
NheI 4069 GCTAGC G^CTAGC
PshAI 3001 GACTGCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 1431 ATTAAT AT^TAAT
SacI 2012 GAGCTC GAGCT^C
SmaI 2633 CCCGGG CCC^GGG
SphI 3894 GCATGC GCATG^C
SspI 2546 AATATT AAT^ATT
XmaCI 2633 CCCGGG C^CCGGG
XmaI 2633 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AcsI 214 GAATTT [GA]^AATT[CT]
520 GAATTC
Aor51HI 2483 AGCGCT AGC^GCT
2621 AGCGCT
ApoI 214 GAATTT [GA]^AATT[CT]
520 GAATTC
AspHI 389 GTGCAC G[AT]GC[AT]^C
2012 GAGCTC
BbeI 56 GGCGCC GGCGC^C
2339 GGCGCC
BglII 267 AGATCT A^GATCT
2852 AGATCT
BsiHKAI 389 GTGCAC G[AT]GC[AT]^C
2012 GAGCTC
BspMI 1662 ACCTGC ACCTGC(4/8)
4244 ACCTGC
BsrBI 2322 CCGCTC CCGCTC(-3/-3)
2859 CCGCTC
BstAPI 3922 GCAGCTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
4183 GCAGCAGCTGC
Eco47III 2483 AGCGCT AGC^GCT
2621 AGCGCT
HgaI 1657 GACGC GACGC(5/10)
2829 GCGTC
HincII 121 GTTGAC GT[CT]^[GA]AC
3642 GTCAAC
HindII 121 GTTGAC GT[CT]^[GA]AC
3642 GTCAAC
KasI 56 GGCGCC G^GCGCC
2339 GGCGCC
MaeII 1635 ACGT A^CGT
1830 ACGT
MslI 15 CATGGAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2122 CACAAAGGTG
NarI 56 GGCGCC GG^CGCC
2339 GGCGCC
NspI 3894 GCATGC [GA]CATG^[CT]
4140 ACATGC
PflMI 354 CCATGAGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3175 CCATCTCTTGG
SfoI 56 GGCGCC GGC^GCC
2339 GGCGCC
Van91I 354 CCATGAGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3175 CCATCTCTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 110 enzymes) #
#########################################
AciI AcyI AfaI AluI AlwI
AlwNI AocI ApaI AvaII BalI
BanI BanII BbsI BbvCI BbvI
BclI BcnI BfaI BmyI BpmI
BpuAI BsaHI BsaJI BseRI BsgI
BsiYI BslI BsmAI BsmFI BsmI
Bsp1286I BsrFI BsrI BssKI BstF5I
BstNI BstXI BstYI Bsu36I Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EarI Eco81I
EcoO109I EcoRII EcoT14I FauI FbaI
Fnu4HI FokI HaeII HaeIII HapII
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MspA1I MspI MvaI
MwoI NciI NcoI NdeII NlaIII
NlaIV PleI PpuMI PstI PvuII
RsaI SapI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XcmI XhoII