Restriction map for
pF1KA0536
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# NO CUT ENZYMES ( 109 enzymes) #
##################################
AatII Acc65I AccIII AcyI AflII
AgeI Alw44I AocI Aor51HI ApaI
ApaLI AscI Asp718I AviII AvrII
BalI BamHI BbeI BbvCI BcgI
BciVI BcnI BfrI BglI BlnI
BlpI Bpu1102I BsaHI BsaWI BseAI
BsgI BsiWI BsmI BspDI BspEI
BsrBI BsrFI BssHII BssSI Bst1107I
BstAPI BstXI BstZ17I Bsu36I CelII
CfoI Cfr10I ClaI CpoI DrdI
EagI EclXI Eco47III Eco52I Eco81I
EcoNI EcoRI EcoT14I EcoT22I FauI
FseI FspI HaeII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HpaI HpaII KasI KpnI MluI
MluNI MroI MscI MspI NaeI
NarI NciI NcoI NgoMIV NheI
NotI NsiI PacI PinAI PmeI
PstI PvuII RsrII SalI SbfI
SfiI SfoI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspI StyI
SwaI XbaI XmaCI XmaI
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# ONE CUT ENZYMES ( 55 enzymes) #
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AclI 2054 AACGTT AA^CGTT
AhdI 910 GACAGAAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AseI 2845 ATTAAT AT^TAAT
AsnI 2845 ATTAAT AT^TAAT
Asp700I 1517 GAAGTCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 910 GACAGAAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 1247 CTCGAG C^[CT]CG[GA]G
BbrPI 1076 CACGTG CAC^GTG
BclI 2769 TGATCA T^GATCA
BsaAI 1076 CACGTG [CT]AC^GT[GA]
BsiEI 858 CGATCG CG[GA][CT]^CG
BsmBI 1218 GAGACG CGTCTC(1/5)
BsmFI 1412 GGGAC GGGAC(10/14)
BsoBI 1247 CTCGAG C^[CT]CG[GA]G
Bsp1407I 2789 TGTACA T^GTACA
BspHI 450 TCATGA T^CATGA
BsrDI 2701 GCAATG GCAATG(2/0)
BsrGI 2789 TGTACA T^GTACA
BstBI 2738 TTCGAA TT^CGAA
BstEII 1019 GGTCACC G^GT[ACGT]ACC
BstPI 1019 GGTCACC G^GT[ACGT]ACC
DraIII 1460 CACGCGGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 8 CCGCGG C^C[GA][CT]GG
EaeI 2 TGGCCG [CT]^GGCC[GA]
Eam1105I 910 GACAGAAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 1019 GGTCACC G^GT[ACGT]ACC
FbaI 2769 TGATCA T^GATCA
KspI 8 CCGCGG CCGC^GG
MfeI 1640 CAATTG C^AATTG
MslI 1680 CATGTCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 1640 CAATTG C^AATTG
NdeI 2686 CATATG CA^TATG
NruI 855 TCGCGA TCG^CGA
NspV 2738 TTCGAA TT^CGAA
PaeR7I 1247 CTCGAG C^TCGAG
PflMI 2133 CCAAGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 1076 CACGTG CAC^GTG
PmlI 1076 CACGTG CAC^GTG
PpuMI 2858 AGGACCT [GA]G^G[AT]CC[CT]
PshBI 2845 ATTAAT AT^TAAT
Psp1406I 2054 AACGTT AA^CGTT
PvuI 858 CGATCG CGAT^CG
RcaI 450 TCATGA T^CATGA
SacI 2167 GAGCTC GAGCT^C
SacII 8 CCGCGG CCGC^GG
SapI 1169 GAAGAGC GCTCTTC(1/4)
ScaI 481 AGTACT AGT^ACT
SexAI 1035 ACCTGGT A^CC[AT]GGT
SfuI 2738 TTCGAA TT^CGAA
SspBI 2789 TGTACA T^GTACA
StuI 1811 AGGCCT AGG^CCT
Van91I 2133 CCAAGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 2129 CCAACCAAGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 1247 CTCGAG C^TCGAG
XmnI 1517 GAAGTCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AccI 1265 GTAGAC GT^[AC][GT]AC
1472 GTAGAC
AflIII 1679 ACATGT A^C[GA][CT]GT
2517 ACATGT
AlwNI 1901 CAGAATCTG CAG[ACGT][ACGT][ACGT]^CTG
2944 CAGATTCTG
BanI 1884 GGCACC G^G[CT][GA]CC
2882 GGTGCC
BanII 400 GGGCTC G[GA]GC[CT]^C
2167 GAGCTC
BspLU11I 1679 ACATGT A^CATGT
2517 ACATGT
BspMI 1097 GCAGGT ACCTGC(4/8)
1373 GCAGGT
BstF5I 677 CATCC GGATG(2/0)
3006 CATCC
DraII 2858 AGGACCT [GA]G^G[ACGT]CC[CT]
2987 AGGCCCT
EcoO109I 2858 AGGACCT [GA]G^G[ACGT]CC[CT]
2987 AGGCCCT
EcoRV 1255 GATATC GAT^ATC
2443 GATATC
FokI 677 CATCC GGATG(9/13)
3006 CATCC
HindIII 2491 AAGCTT A^AGCTT
2695 AAGCTT
MspA1I 8 CCGCGG C[AC]G^C[GT]G
1952 CCGCTG
PleI 2255 GACTC GAGTC(4/5)
421 GAGTC
PshAI 578 GACATAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
1414 GACAGAGGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 78 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI ApoI AspHI AspI AvaII
BbsI BbvI BfaI BglII BmyI
BpmI BpuAI BsaBI BsaI BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
Bsp1286I BsrI BssKI BstNI BstUI
BstYI Cac8I Cfr13I DdeI DpnII
DraI EarI EcoRII Fnu4HI HaeIII
HinfI HphI ItaI Ksp632I MaeI
MaeII MaeIII MamI MboI MboII
MflI MnlI MseI MvaI MvnI
MwoI NdeII NlaIII NlaIV NspI
PflFI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
Tth111I TthHB8I XhoII