Restriction map for
pF1KA0531
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# NO CUT ENZYMES ( 87 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcyI AflII AgeI AhdI AscI
AseI AsnI Asp700I Asp718I AspEI
AviII AvrII BbeI BbvCI BclI
BfrI BlnI BsaHI BsaWI BseAI
BsiWI BspDI BspEI BsrDI BssSI
Bst1107I BstBI BstEII BstPI BstZ17I
ClaI CpoI DraIII DrdI Eam1105I
EcoNI EcoO65I EcoRI FbaI FseI
FspI Hin1I HincII HindII HpaI
KasI KpnI MfeI MluI MroI
MunI NaeI NarI NgoMIV NheI
NotI NruI NspV PacI PinAI
PmeI PpuMI PshAI PshBI Psp1406I
PvuI RsrII SacI SalI SbfI
ScaI SexAI SfoI SfuI SgrAI
SnaBI SpeI SphI Sse8387I StuI
SwaI XmnI
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# ONE CUT ENZYMES ( 50 enzymes) #
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Alw44I 504 GTGCAC G^TGCAC
Aor51HI 2684 AGCGCT AGC^GCT
ApaI 2747 GGGCCC GGGCC^C
ApaLI 504 GTGCAC G^TGCAC
AspHI 504 GTGCAC G[AT]GC[AT]^C
BalI 2738 TGGCCA TGG^CCA
BamHI 6 GGATCC G^GATCC
BbrPI 574 CACGTG CAC^GTG
BciVI 445 GTATCC GTATCC(6/5)
BsaAI 574 CACGTG [CT]AC^GT[GA]
BsaBI 2209 GATTTGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsgI 2392 CTGCAC GTGCAG(16/14)
BsiEI 2810 CGGCCG CG[GA][CT]^CG
BsiHKAI 504 GTGCAC G[AT]GC[AT]^C
Bsp1407I 1766 TGTACA T^GTACA
BspHI 468 TCATGA T^CATGA
BspLU11I 825 ACATGT A^CATGT
BsrFI 38 GCCGGT [GA]^CCGG[CT]
BsrGI 1766 TGTACA T^GTACA
BssHII 2642 GCGCGC G^CGCGC
BstAPI 2385 GCAGACACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 2729 CCAAGAACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 38 GCCGGT [GA]^CCGG[CT]
DraI 97 TTTAAA TTT^AAA
EagI 2810 CGGCCG C^GGCCG
EclXI 2810 CGGCCG C^GGCCG
Eco47III 2684 AGCGCT AGC^GCT
Eco52I 2810 CGGCCG C^GGCCG
EcoRV 343 GATATC GAT^ATC
EcoT22I 191 ATGCAT ATGCA^T
HindIII 2242 AAGCTT A^AGCTT
KspI 72 CCGCGG CCGC^GG
MamI 2209 GATTTGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 2738 TGGCCA TGG^CCA
MscI 2738 TGGCCA TGG^CCA
NdeI 137 CATATG CA^TATG
NsiI 191 ATGCAT ATGCA^T
PaeR7I 1822 CTCGAG C^TCGAG
PmaCI 574 CACGTG CAC^GTG
PmlI 574 CACGTG CAC^GTG
PstI 885 CTGCAG CTGCA^G
RcaI 468 TCATGA T^CATGA
SacII 72 CCGCGG CCGC^GG
SmaI 1526 CCCGGG CCC^GGG
SmlI 1822 CTCGAG C^T[CT][GA]AG
SspBI 1766 TGTACA T^GTACA
XcmI 449 CCAAGACCAACTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 1822 CTCGAG C^TCGAG
XmaCI 1526 CCCGGG C^CCGGG
XmaI 1526 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 28 enzymes) #
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AfaI 1248 GTAC GT^AC
1767 GTAC
AocI 529 CCTGAGG CC^T[ACGT]AGG
1129 CCTGAGG
AspI 939 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2367 GACAGTGTC
BanI 727 GGTGCC G^G[CT][GA]CC
1696 GGCACC
BcgI 1814 GCAAACAGCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2394 GCACAACCTTCG
BglI 2719 GCCGTGCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2740 GCCAGAAGGGC
BsaI 109 GAGACC GGTCTC(1/5)
937 GAGACC
BsmBI 1414 CGTCTC CGTCTC(1/5)
2165 GAGACG
BsmI 16 GAATGC GAATGC(1/-1)
1857 GAATGC
BspMI 2570 ACCTGC ACCTGC(4/8)
180 GCAGGT
BsrBI 511 GAGCGG CCGCTC(-3/-3)
1867 GAGCGG
Bsu36I 529 CCTGAGG CC^T[ACGT]AGG
1129 CCTGAGG
DraII 1466 AGGCCCT [GA]G^G[ACGT]CC[CT]
2746 AGGGCCC
EaeI 2738 TGGCCA [CT]^GGCC[GA]
2810 CGGCCG
Eco81I 529 CCTGAGG CC^T[ACGT]AGG
1129 CCTGAGG
EcoO109I 1466 AGGCCCT [GA]G^G[ACGT]CC[CT]
2746 AGGGCCC
HgaI 2070 GACGC GACGC(5/10)
2624 GCGTC
MaeII 575 ACGT A^CGT
1413 ACGT
MaeIII 583 GTGAC ^GT[ACGT]AC
614 GTCAC
MslI 24 CATCAAAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
948 CACACTGATG
PflFI 939 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2367 GACAGTGTC
RsaI 1248 GTAC GT^AC
1767 GTAC
SapI 1996 GAAGAGC GCTCTTC(1/4)
2843 GCTCTTC
SfiI 2718 GGCCGTGCGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
2739 GGCCAGAAGGGCC
SspI 634 AATATT AAT^ATT
1201 AATATT
Tsp45I 583 GTGAC ^GT[GC]AC
614 GTCAC
Tth111I 939 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2367 GACAGTGTC
XbaI 607 TCTAGA T^CTAGA
2374 TCTAGA
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# 3 OR MORE CUT ENZYMES ( 93 enzymes) #
#########################################
AccII AciI AcsI AflIII AluI
AlwI AlwNI ApoI AvaI AvaII
BanII BbsI BbvI BcnI BfaI
BglII BlpI BmyI BpmI Bpu1102I
BpuAI BsaJI BseRI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BsrI
BssKI BstF5I BstNI BstUI BstYI
Cac8I CelII CfoI Cfr13I DdeI
DpnII DsaI EarI EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MboI
MboII MflI MnlI MseI MspA1I
MspI MvaI MvnI MwoI NciI
NcoI NdeII NlaIII NlaIV NspI
PflMI PleI PvuII Sau3AI Sau96I
ScrFI SfaNI SfcI StyI TaqI
TfiI Tru9I TseI Tsp509I TspRI
TthHB8I Van91I XhoII