Restriction map for
pF1KA0425
##################################
# NO CUT ENZYMES ( 105 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AflII AgeI Alw44I ApaI
ApaLI AscI AseI AsnI Asp718I
AspI AvaI AviII AvrII BamHI
BbeI BbrPI BcgI BciVI BfrI
BglI BglII BlnI BsaBI BsaHI
BsaWI BseAI BsiEI BsiWI BsoBI
BspDI BspEI BspHI BsrFI BssHII
BssSI BstBI BstEII BstPI BstUI
Cfr10I ClaI CpoI DrdI EagI
EclXI Eco52I EcoO65I EcoRV FseI
FspI Hin1I KasI KpnI KspI
MamI MfeI MluI MroI MunI
MvnI NaeI NarI NgoMIV NheI
NotI NruI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacI SacII SalI SbfI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SphI Sse8387I SwaI
Tth111I XcmI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AhdI 2848 GACATTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 2765 CCTTAGG CC^T[ACGT]AGG
Aor51HI 3515 AGCGCT AGC^GCT
AspEI 2848 GACATTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 3220 GAGCAC G[AT]GC[AT]^C
BalI 1466 TGGCCA TGG^CCA
BanI 1971 GGTGCC G^G[CT][GA]CC
BclI 233 TGATCA T^GATCA
BcnI 2630 CCGGG CC^[GC]GG
BlpI 1720 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1720 GCTCAGC GC^T[ACGT]AGC
BsaAI 3593 CACGTA [CT]AC^GT[GA]
BsiHKAI 3220 GAGCAC G[AT]GC[AT]^C
Bsp1407I 750 TGTACA T^GTACA
BsrBI 2826 CCGCTC CCGCTC(-3/-3)
BsrDI 1207 GCAATG GCAATG(2/0)
BsrGI 750 TGTACA T^GTACA
Bst1107I 821 GTATAC GTA^TAC
BstAPI 827 GCAACTTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstZ17I 821 GTATAC GTA^TAC
Bsu36I 2765 CCTTAGG CC^T[ACGT]AGG
CelII 1720 GCTCAGC GC^T[ACGT]AGC
DsaI 1625 CCATGG C^C[GA][CT]GG
EaeI 1466 TGGCCA [CT]^GGCC[GA]
Eam1105I 2848 GACATTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 3515 AGCGCT AGC^GCT
Eco81I 2765 CCTTAGG CC^T[ACGT]AGG
EcoNI 69 CCTCCAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 1431 ATGCAT ATGCA^T
FbaI 233 TGATCA T^GATCA
HgaI 1029 GCGTC GACGC(5/10)
HpaI 3588 GTTAAC GTT^AAC
MluNI 1466 TGGCCA TGG^CCA
MscI 1466 TGGCCA TGG^CCA
NciI 2630 CCGGG CC^[GC]GG
NcoI 1625 CCATGG C^CATGG
NdeI 299 CATATG CA^TATG
NsiI 1431 ATGCAT ATGCA^T
PflMI 405 CCAGAATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SpeI 262 ACTAGT A^CTAGT
SspBI 750 TGTACA T^GTACA
StuI 2738 AGGCCT AGG^CCT
Van91I 405 CCAGAATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 2256 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AccI 105 GTCTAC GT^[AC][GT]AC
821 GTATAC
BanII 2584 GAGCCC G[GA]GC[CT]^C
3016 GAGCCC
BbvCI 644 CCTCAGC CCTCAGC(-5/-2)
779 CCTCAGC
BsaI 2913 GAGACC GGTCTC(1/5)
1021 GGTCTC
BseRI 173 CTCCTC GAGGAG(10/8)
965 GAGGAG
BsmBI 1030 CGTCTC CGTCTC(1/5)
3252 GAGACG
BspLU11I 2648 ACATGT A^CATGT
3092 ACATGT
BstYI 640 AGATCC [GA]^GATC[CT]
2712 GGATCT
Cac8I 160 GCCCGC GC[ACGT]^[ACGT]GC
436 GCCTGC
DraI 216 TTTAAA TTT^AAA
3165 TTTAAA
DraII 1647 AGGACCC [GA]G^G[ACGT]CC[CT]
2435 AGGACCT
EcoO109I 1647 AGGACCC [GA]G^G[ACGT]CC[CT]
2435 AGGACCT
FauI 161 CCCGC CCCGC(4/6)
1593 CCCGC
HaeII 3142 GGCGCT [GA]GCGC^[CT]
3515 AGCGCT
HapII 962 CCGG C^CGG
2630 CCGG
HpaII 962 CCGG C^CGG
2630 CCGG
MflI 640 AGATCC [GA]^GATC[CT]
2712 GGATCT
MslI 2524 CACAGAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
3422 CACTAAGATG
MspI 962 CCGG C^CGG
2630 CCGG
PpuMI 1647 AGGACCC [GA]G^G[AT]CC[CT]
2435 AGGACCT
ScaI 1798 AGTACT AGT^ACT
2773 AGTACT
SspI 1217 AATATT AAT^ATT
1618 AATATT
Tsp45I 1589 GTGAC ^GT[GC]AC
2107 GTCAC
XhoII 640 AGATCC [GA]^GATC[CT]
2712 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 85 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI AlwNI ApoI Asp700I AvaII
BbsI BbvI BfaI BmyI BpmI
BpuAI BsaJI BsgI BsiYI BslI
BsmAI BsmFI BsmI Bsp1286I BspMI
BsrI BssKI BstF5I BstNI BstXI
CfoI Cfr13I DdeI DpnII DraIII
EarI EcoRI EcoRII EcoT14I Fnu4HI
FokI HaeIII HhaI HinP1I HincII
HindII HindIII HinfI HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MnlI MseI MspA1I MvaI
MwoI NdeII NlaIII NlaIV NspI
PleI PstI PvuII RsaI SapI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StyI TaqI TfiI Tru9I
TseI Tsp509I TspRI TthHB8I XmnI