Restriction map for
pF1KA0419
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# NO CUT ENZYMES ( 131 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I Aor51HI ApaI ApaLI AscI
AseI AsnI Asp718I AspEI AspHI
AvaI AviII AvrII BamHI BanI
BanII BbeI BbrPI BbvCI BcgI
BciVI BcnI BfrI BglI BglII
BlnI BpmI BsaAI BsaHI BsaWI
BseAI BsgI BsiEI BsiHKAI BsiWI
BsmBI BsoBI Bsp1407I BspDI BspEI
BspLU11I BsrBI BsrFI BsrGI BssHII
Bst1107I BstEII BstPI BstUI BstXI
BstZ17I Cfr10I ClaI CpoI DraII
DraIII DrdI DsaI EagI Eam1105I
EclXI Eco47III Eco52I EcoNI EcoO109I
EcoO65I EcoRV EcoT22I FseI FspI
HaeII HapII Hin1I HpaII KasI
KpnI KspI MfeI MluI MroI
MspI MunI MvnI NaeI NarI
NciI NcoI NgoMIV NotI NruI
NsiI NspI PacI PaeR7I PinAI
PmaCI PmeI PmlI PpuMI PshBI
Psp1406I PvuI RsrII SacI SacII
SalI SbfI ScaI SexAI SfiI
SfoI SgrAI SmaI SnaBI SphI
Sse8387I SspBI SwaI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AocI 1864 CCTAAGG CC^T[ACGT]AGG
AspI 734 GACGCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 987 TGGCCA TGG^CCA
BclI 300 TGATCA T^GATCA
BlpI 2014 GCTGAGC GC^T[ACGT]AGC
BmyI 1261 GTGCCC G[AGT]GC[ACT]^C
Bpu1102I 2014 GCTGAGC GC^T[ACGT]AGC
BsaBI 295 GATTTTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 2360 GGTCTC GGTCTC(1/5)
BsmFI 608 GTCCC GGGAC(10/14)
Bsp1286I 1261 GTGCCC G[AGT]GC[ACT]^C
BspMI 210 GCAGGT ACCTGC(4/8)
BstBI 960 TTCGAA TT^CGAA
Bsu36I 1864 CCTAAGG CC^T[ACGT]AGG
CelII 2014 GCTGAGC GC^T[ACGT]AGC
CfoI 1469 GCGC GCG^C
DraI 1021 TTTAAA TTT^AAA
Eco81I 1864 CCTAAGG CC^T[ACGT]AGG
EcoRI 2494 GAATTC G^AATTC
FauI 1297 GCGGG CCCGC(4/6)
FbaI 300 TGATCA T^GATCA
HgaI 734 GACGC GACGC(5/10)
HhaI 1469 GCGC GCG^C
HinP1I 1469 GCGC G^CGC
HphI 2231 TCACC GGTGA(8/7)
MaeII 1134 ACGT A^CGT
MamI 295 GATTTTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 987 TGGCCA TGG^CCA
MscI 987 TGGCCA TGG^CCA
NheI 952 GCTAGC G^CTAGC
NlaIV 607 GGTCCC GG[ACGT]^[ACGT]CC
NspV 960 TTCGAA TT^CGAA
PflFI 734 GACGCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1549 CCAAACTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 237 GACTCGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SfuI 960 TTCGAA TT^CGAA
SspI 532 AATATT AAT^ATT
StuI 2332 AGGCCT AGG^CCT
Tsp45I 2156 GTGAC ^GT[GC]AC
Tth111I 734 GACGCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1549 CCAAACTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 2278 CCATTCTACCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 29 enzymes) #
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AccI 2435 GTAGAC GT^[AC][GT]AC
2891 GTAGAC
AfaI 1487 GTAC GT^AC
2601 GTAC
AlwNI 685 CAGAATCTG CAG[ACGT][ACGT][ACGT]^CTG
1485 CAGTACCTG
AvaII 453 GGACC G^G[AT]CC
607 GGTCC
BsaJI 906 CCAAGG C^C[ACGT][ACGT]GG
2856 CCAAGG
BseRI 4 GAGGAG GAGGAG(10/8)
1924 GAGGAG
BsiYI 1549 CCAAACTTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
2851 CCAAACCAAGG
BslI 1549 CCAAACTTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
2851 CCAAACCAAGG
BsmI 2792 GAATGC GAATGC(1/-1)
2865 GAATGC
BspHI 2610 TCATGA T^CATGA
2661 TCATGA
BssKI 246 CCAGG ^CC[ACGT]GG
1139 CCAGG
BssSI 2720 CACGAG CACGAG(-5/-1)
239 CTCGTG
BstAPI 280 GCACCTAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1839 GCAACATTTGC
BstNI 246 CCAGG CC^[AT]GG
1139 CCAGG
Cfr13I 453 GGACC G^G[ACGT]CC
607 GGTCC
EaeI 987 TGGCCA [CT]^GGCC[GA]
2930 CGGCCA
EcoRII 246 CCAGG ^CC[AT]GG
1139 CCAGG
EcoT14I 906 CCAAGG C^C[AT][AT]GG
2856 CCAAGG
MvaI 246 CCAGG CC^[AT]GG
1139 CCAGG
NdeI 1079 CATATG CA^TATG
1520 CATATG
PvuII 2636 CAGCTG CAG^CTG
2735 CAGCTG
RcaI 2610 TCATGA T^CATGA
2661 TCATGA
RsaI 1487 GTAC GT^AC
2601 GTAC
SapI 2056 GAAGAGC GCTCTTC(1/4)
2342 GAAGAGC
Sau96I 453 GGACC G^G[ACGT]CC
607 GGTCC
ScrFI 246 CCAGG CC^[ACGT]GG
1139 CCAGG
SpeI 1906 ACTAGT A^CTAGT
2302 ACTAGT
StyI 906 CCAAGG C^C[AT][AT]GG
2856 CCAAGG
XbaI 730 TCTAGA T^CTAGA
1002 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 56 enzymes) #
#########################################
AciI AcsI AluI AlwI ApoI
Asp700I BbsI BbvI BfaI BpuAI
BsmAI BsrDI BsrI BstF5I BstYI
Cac8I DdeI DpnII EarI Fnu4HI
FokI HaeIII HincII HindII HindIII
HinfI HpaI ItaI Ksp632I MaeI
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MwoI NdeII
NlaIII PleI PstI Sau3AI SfaNI
SfcI SmlI TaqI TfiI Tru9I
TseI Tsp509I TspRI TthHB8I XhoII
XmnI