Restriction map for
pF1KA0406
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# NO CUT ENZYMES ( 106 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AflII AgeI Alw44I Aor51HI
ApaLI AscI Asp700I Asp718I AspI
AvaI AviII BamHI BbeI BbrPI
BciVI BcnI BfrI BlpI Bpu1102I
BseAI BsiEI BsiWI BsmBI BsoBI
Bsp1407I BspDI BspEI BspHI BsrBI
BsrGI BssHII BssSI Bst1107I BstBI
BstUI BstXI BstZ17I CelII CfoI
ClaI CpoI DrdI EagI EclXI
Eco47III Eco52I EcoRI EcoRV FseI
FspI HaeII HhaI HinP1I HindIII
HpaI KasI KpnI KspI MfeI
MluI MroI MunI MvnI NaeI
NarI NciI NgoMIV NheI NotI
NruI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI Psp1406I
PvuI RcaI RsrII SacI SacII
SalI SbfI SfiI SfoI SfuI
SgrAI SmaI Sse8387I SspBI SspI
SwaI Tth111I XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AcyI 2761 GACGCC G[GA]^CG[CT]C
AflIII 253 ACATGT A^C[GA][CT]GT
AocI 19 CCTGAGG CC^T[ACGT]AGG
AseI 387 ATTAAT AT^TAAT
AsnI 387 ATTAAT AT^TAAT
AvrII 2994 CCTAGG C^CTAGG
BanI 2333 GGCACC G^G[CT][GA]CC
BbvCI 1385 CCTCAGC CCTCAGC(-5/-2)
BcgI 3233 CGACCAACGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 2069 TGATCA T^GATCA
BglI 2764 GCCCCCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 710 AGATCT A^GATCT
BlnI 2994 CCTAGG C^CTAGG
BsaAI 2798 TACGTA [CT]AC^GT[GA]
BsaBI 2626 GATAAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 2761 GACGCC G[GA]^CG[CT]C
BsaI 1982 GAGACC GGTCTC(1/5)
BsaWI 1553 ACCGGA [AT]^CCGG[AT]
BspLU11I 253 ACATGT A^CATGT
BsrDI 2570 CATTGC GCAATG(2/0)
BsrFI 2837 GCCGGT [GA]^CCGG[CT]
BstAPI 778 GCAAAACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 19 CCTGAGG CC^T[ACGT]AGG
Cfr10I 2837 GCCGGT [GA]^CCGG[CT]
DraIII 3187 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 2588 CCATGG C^C[GA][CT]GG
Eco81I 19 CCTGAGG CC^T[ACGT]AGG
EcoT22I 1881 ATGCAT ATGCA^T
FauI 3212 GCGGG CCCGC(4/6)
FbaI 2069 TGATCA T^GATCA
Hin1I 2761 GACGCC G[GA]^CG[CT]C
HincII 858 GTTGAC GT[CT]^[GA]AC
HindII 858 GTTGAC GT[CT]^[GA]AC
MamI 2626 GATAAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NcoI 2588 CCATGG C^CATGG
NdeI 1913 CATATG CA^TATG
NsiI 1881 ATGCAT ATGCA^T
PshAI 2539 GACACCCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 387 ATTAAT AT^TAAT
SapI 2400 GAAGAGC GCTCTTC(1/4)
ScaI 1965 AGTACT AGT^ACT
SexAI 3124 ACCTGGT A^CC[AT]GGT
SnaBI 2798 TACGTA TAC^GTA
SpeI 1014 ACTAGT A^CTAGT
SphI 2280 GCATGC GCATG^C
XbaI 1305 TCTAGA T^CTAGA
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# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AcsI 1189 AAATTC [GA]^AATT[CT]
2079 AAATTC
AhdI 183 GACCCCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1114 GACATCTTGTC
ApaI 1233 GGGCCC GGGCC^C
2970 GGGCCC
ApoI 1189 AAATTC [GA]^AATT[CT]
2079 AAATTC
AspEI 183 GACCCCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1114 GACATCTTGTC
BalI 1902 TGGCCA TGG^CCA
2225 TGGCCA
BstEII 1716 GGTTACC G^GT[ACGT]ACC
3004 GGTGACC
BstPI 1716 GGTTACC G^GT[ACGT]ACC
3004 GGTGACC
DraI 673 TTTAAA TTT^AAA
2109 TTTAAA
EaeI 1902 TGGCCA [CT]^GGCC[GA]
2225 TGGCCA
Eam1105I 183 GACCCCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1114 GACATCTTGTC
EcoNI 993 CCTTCTGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2994 CCTAGGTGAGG
EcoO65I 1716 GGTTACC G^GT[ACGT]ACC
3004 GGTGACC
HapII 1554 CCGG C^CGG
2838 CCGG
HpaII 1554 CCGG C^CGG
2838 CCGG
MluNI 1902 TGGCCA TGG^CCA
2225 TGGCCA
MscI 1902 TGGCCA TGG^CCA
2225 TGGCCA
MspI 1554 CCGG C^CGG
2838 CCGG
NspI 253 ACATGT [GA]CATG^[CT]
2280 GCATGC
PflMI 72 CCAGACAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
213 CCAAAGTGTGG
TaqI 977 TCGA T^CGA
2426 TCGA
TthHB8I 977 TCGA T^CGA
2426 TCGA
Van91I 72 CCAGACAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
213 CCAAAGTGTGG
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# 3 OR MORE CUT ENZYMES ( 83 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AspHI AvaII BanII BbsI BbvI
BfaI BmyI BpmI BpuAI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI Bsp1286I BspMI
BsrI BssKI BstF5I BstNI BstYI
Cac8I Cfr13I DdeI DpnII DraII
EarI EcoO109I EcoRII EcoT14I Fnu4HI
FokI HaeIII HgaI HinfI HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MvaI MwoI NdeII
NlaIII NlaIV PleI PpuMI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StuI StyI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI XcmI XhoII