Restriction map for
pF1KA0399
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# NO CUT ENZYMES ( 50 enzymes) #
##################################
AccIII AclI AflII AhdI AscI
AseI AsnI Asp700I AspEI AspI
AviII BfrI BseAI BsiWI BspEI
BssHII Bst1107I BstZ17I CpoI Eam1105I
EcoRV EcoT22I FseI FspI HpaI
MfeI MluI MroI MunI NotI
NruI NsiI PacI PflFI PmeI
PshBI Psp1406I PvuI RsrII SalI
SbfI SfiI SgrAI SnaBI SpeI
Sse8387I SwaI Tth111I XbaI XmnI
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# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AatII 4675 GACGTC GACGT^C
Acc65I 2102 GGTACC G^GTACC
AccI 6454 GTAGAC GT^[AC][GT]AC
AgeI 5931 ACCGGT A^CCGGT
Asp718I 2102 GGTACC G^GTACC
AvrII 5430 CCTAGG C^CTAGG
BbeI 322 GGCGCC GGCGC^C
BbvCI 373 GCTGAGG CCTCAGC(-5/-2)
BlnI 5430 CCTAGG C^CTAGG
BsaBI 2769 GATGAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 8384 CGGCCG CG[GA][CT]^CG
Bsp1407I 2492 TGTACA T^GTACA
BspDI 5378 ATCGAT AT^CGAT
BsrBI 256 GAGCGG CCGCTC(-3/-3)
BsrGI 2492 TGTACA T^GTACA
BssSI 6736 CTCGTG CACGAG(-5/-1)
BstBI 4142 TTCGAA TT^CGAA
BstEII 5637 GGTAACC G^GT[ACGT]ACC
BstPI 5637 GGTAACC G^GT[ACGT]ACC
ClaI 5378 ATCGAT AT^CGAT
DraI 867 TTTAAA TTT^AAA
DrdI 2800 GACACTCTCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 8384 CGGCCG C^GGCCG
EclXI 8384 CGGCCG C^GGCCG
Eco52I 8384 CGGCCG C^GGCCG
EcoO65I 5637 GGTAACC G^GT[ACGT]ACC
HindIII 1879 AAGCTT A^AGCTT
KasI 322 GGCGCC G^GCGCC
KpnI 2102 GGTACC GGTAC^C
MamI 2769 GATGAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NarI 322 GGCGCC GG^CGCC
NdeI 7942 CATATG CA^TATG
NheI 6142 GCTAGC G^CTAGC
NspV 4142 TTCGAA TT^CGAA
PinAI 5931 ACCGGT A^CCGGT
ScaI 6962 AGTACT AGT^ACT
SexAI 2237 ACCTGGT A^CC[AT]GGT
SfoI 322 GGCGCC GGC^GCC
SfuI 4142 TTCGAA TT^CGAA
SmaI 108 CCCGGG CCC^GGG
SspBI 2492 TGTACA T^GTACA
SspI 2024 AATATT AAT^ATT
XmaCI 108 CCCGGG C^CCGGG
XmaI 108 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 12 enzymes) #
###################################
Aor51HI 126 AGCGCT AGC^GCT
2069 AGCGCT
BamHI 2721 GGATCC G^GATCC
6099 GGATCC
BbrPI 7861 CACGTG CAC^GTG
7897 CACGTG
BsmI 969 GAATGC GAATGC(1/-1)
2353 GAATGC
Eco47III 126 AGCGCT AGC^GCT
2069 AGCGCT
PaeR7I 2822 CTCGAG C^TCGAG
5907 CTCGAG
PmaCI 7861 CACGTG CAC^GTG
7897 CACGTG
PmlI 7861 CACGTG CAC^GTG
7897 CACGTG
PshAI 3532 GACAGCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
4456 GACCAGAGTC
SphI 5816 GCATGC GCATG^C
8062 GCATGC
XcmI 67 CCACACCAGGACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2021 CCAAATATTTGATGG
XhoI 2822 CTCGAG C^TCGAG
5907 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 152 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AluI Alw44I AlwI AlwNI
AocI ApaI ApaLI ApoI AspHI
AvaI AvaII BalI BanI BanII
BbsI BbvI BcgI BciVI BclI
BcnI BfaI BglI BglII BlpI
BmyI BpmI Bpu1102I BpuAI BsaAI
BsaHI BsaI BsaJI BsaWI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmBI BsmFI BsoBI Bsp1286I BspHI
BspLU11I BspMI BsrDI BsrFI BsrI
BssKI BstAPI BstF5I BstNI BstUI
BstXI BstYI Bsu36I Cac8I CelII
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DraIII DsaI EaeI EarI
Eco81I EcoNI EcoO109I EcoRI EcoRII
EcoT14I FauI FbaI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HincII HindII HinfI
HpaII HphI ItaI Ksp632I KspI
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NaeI NciI NcoI NdeII
NgoMIV NlaIII NlaIV NspI PflMI
PleI PpuMI PstI PvuII RcaI
RsaI SacI SacII SapI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XhoII