Restriction map for
pF1KA0391
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# NO CUT ENZYMES ( 135 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflIII AgeI Alw44I
AlwNI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp700I Asp718I AspI
AvaI AviII BamHI BbeI BbrPI
BbvCI BcgI BclI BglI BglII
BlpI Bpu1102I BsaAI BsaBI BsaHI
BseAI BsiEI BsiWI BsmI BsoBI
Bsp1407I BspDI BspEI BspLU11I BsrBI
BsrDI BsrFI BsrGI BssHII Bst1107I
BstAPI BstUI BstYI BstZ17I CelII
CfoI Cfr10I ClaI CpoI DraII
DraIII DrdI DsaI EagI EclXI
Eco47III Eco52I EcoNI EcoO109I EcoT22I
FbaI FseI FspI HaeII HgaI
HhaI Hin1I HinP1I HincII HindII
HpaI KasI KpnI KspI MaeII
MamI MflI MluI MroI MvnI
NaeI NarI NcoI NgoMIV NheI
NotI NruI NsiI PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
RsrII SacII SalI SbfI ScaI
SexAI SfiI SfoI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SspI SwaI Tth111I XbaI XcmI
XhoI XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 38 enzymes) #
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AhdI 1268 GACTGCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 1114 CCTCAGG CC^T[ACGT]AGG
AspEI 1268 GACTGCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 758 GAGCTC G[AT]GC[AT]^C
AvrII 1278 CCTAGG C^CTAGG
BalI 987 TGGCCA TGG^CCA
BanI 315 GGCACC G^G[CT][GA]CC
BlnI 1278 CCTAGG C^CTAGG
BsaWI 403 ACCGGA [AT]^CCGG[AT]
BseRI 364 GAGGAG GAGGAG(10/8)
BsgI 1411 CTGCAC GTGCAG(16/14)
BsiHKAI 758 GAGCTC G[AT]GC[AT]^C
BsmBI 1239 CGTCTC CGTCTC(1/5)
BspMI 1430 GCAGGT ACCTGC(4/8)
BssSI 1614 CTCGTG CACGAG(-5/-1)
BstEII 1090 GGTGACC G^GT[ACGT]ACC
BstPI 1090 GGTGACC G^GT[ACGT]ACC
BstXI 1669 CCAACCAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 1114 CCTCAGG CC^T[ACGT]AGG
EaeI 987 TGGCCA [CT]^GGCC[GA]
Eam1105I 1268 GACTGCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 1114 CCTCAGG CC^T[ACGT]AGG
EcoO65I 1090 GGTGACC G^GT[ACGT]ACC
EcoRV 197 GATATC GAT^ATC
FauI 1455 GCGGG CCCGC(4/6)
HphI 1090 GGTGA GGTGA(8/7)
MfeI 1532 CAATTG C^AATTG
MluNI 987 TGGCCA TGG^CCA
MscI 987 TGGCCA TGG^CCA
MunI 1532 CAATTG C^AATTG
NspI 1292 ACATGC [GA]CATG^[CT]
PflMI 52 CCATACCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 1428 CTGCAG CTGCA^G
SacI 758 GAGCTC GAGCT^C
SapI 45 GAAGAGC GCTCTTC(1/4)
StuI 1466 AGGCCT AGG^CCT
Tsp45I 1091 GTGAC ^GT[GC]AC
Van91I 52 CCATACCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AciI 105 CCGC CCGC(-3/-1)
1455 GCGG
AfaI 1098 GTAC GT^AC
1666 GTAC
AvaII 1275 GGTCC G^G[AT]CC
1458 GGACC
BanII 48 GAGCCC G[GA]GC[CT]^C
758 GAGCTC
BbsI 1636 GAAGAC GAAGAC(2/6)
641 GTCTTC
BciVI 749 GTATCC GTATCC(6/5)
939 GTATCC
BcnI 651 CCGGG CC^[GC]GG
1418 CCGGG
BpmI 260 CTGGAG CTGGAG(16/14)
1607 CTGGAG
BpuAI 1636 GAAGAC GAAGAC(2/6)
641 GTCTTC
BsaI 1445 GAGACC GGTCTC(1/5)
1180 GGTCTC
BsmFI 1457 GGGAC GGGAC(10/14)
1518 GGGAC
BspHI 816 TCATGA T^CATGA
1067 TCATGA
BstBI 23 TTCGAA TT^CGAA
418 TTCGAA
DraI 390 TTTAAA TTT^AAA
843 TTTAAA
EarI 45 GAAGAG CTCTTC(1/4)
1333 GAAGAG
EcoRI 288 GAATTC G^AATTC
357 GAATTC
Ksp632I 45 GAAGAG CTCTTC(1/4)
1333 GAAGAG
NciI 651 CCGGG CC^[GC]GG
1418 CCGGG
NdeI 562 CATATG CA^TATG
1628 CATATG
NspV 23 TTCGAA TT^CGAA
418 TTCGAA
RcaI 816 TCATGA T^CATGA
1067 TCATGA
RsaI 1098 GTAC GT^AC
1666 GTAC
SfcI 461 CTATAG C^T[GA][CT]AG
1428 CTGCAG
SfuI 23 TTCGAA TT^CGAA
418 TTCGAA
TaqI 24 TCGA T^CGA
419 TCGA
TthHB8I 24 TCGA T^CGA
419 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 59 enzymes) #
#########################################
AcsI AflII AluI AlwI ApoI
BbvI BfaI BfrI BmyI BsaJI
BsiYI BslI BsmAI Bsp1286I BsrI
BssKI BstF5I BstNI Cac8I Cfr13I
DdeI DpnII EcoRII EcoT14I Fnu4HI
FokI HaeIII HapII HindIII HinfI
HpaII ItaI MaeI MaeIII MboI
MboII MnlI MseI MslI MspA1I
MspI MvaI MwoI NdeII NlaIII
NlaIV PleI PvuII Sau3AI Sau96I
ScrFI SfaNI SmlI StyI TfiI
Tru9I TseI Tsp509I TspRI