Restriction map for
pF1KA0324
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# NO CUT ENZYMES ( 62 enzymes) #
##################################
Acc65I AccI AflIII AgeI Alw44I
Aor51HI ApaLI AscI AseI AsnI
Asp718I AviII BbrPI BciVI BsiEI
BsiWI BspDI BspLU11I BssHII Bst1107I
BstBI BstZ17I ClaI CpoI DraI
EagI EclXI Eco47III Eco52I EcoRV
EcoT22I FseI FspI HpaI KpnI
KspI MfeI MluI MunI NcoI
NdeI NheI NotI NruI NsiI
NspV PacI PinAI PmaCI PmeI
PmlI PshBI PvuI RsrII SacII
SalI SbfI ScaI SfiI SfuI
Sse8387I SwaI
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# ONE CUT ENZYMES ( 36 enzymes) #
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AclI 6119 AACGTT AA^CGTT
AflII 1155 CTTAAG C^TTAAG
AhdI 8059 GACTCTCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 8059 GACTCTCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 4510 GAGCTC G[AT]GC[AT]^C
AvrII 4477 CCTAGG C^CTAGG
BalI 6809 TGGCCA TGG^CCA
BfrI 1155 CTTAAG C^TTAAG
BlnI 4477 CCTAGG C^CTAGG
BsaAI 433 TACGTA [CT]AC^GT[GA]
BsiHKAI 4510 GAGCTC G[AT]GC[AT]^C
BsmI 6401 GAATGC GAATGC(1/-1)
Bsp1407I 2 TGTACA T^GTACA
BspHI 6650 TCATGA T^CATGA
BsrDI 3683 CATTGC GCAATG(2/0)
BsrGI 2 TGTACA T^GTACA
BstAPI 6700 GCAGCCTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
DraIII 4841 CACAGAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 2027 GACGCAGTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 8059 GACTCTCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 3450 GAATTC G^AATTC
MluNI 6809 TGGCCA TGG^CCA
MscI 6809 TGGCCA TGG^CCA
PflMI 8002 CCACCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Psp1406I 6119 AACGTT AA^CGTT
RcaI 6650 TCATGA T^CATGA
SacI 4510 GAGCTC GAGCT^C
SgrAI 5617 CACCGGCG C[GA]^CCGG[CT]G
SmaI 29 CCCGGG CCC^GGG
SnaBI 433 TACGTA TAC^GTA
SpeI 3072 ACTAGT A^CTAGT
SspBI 2 TGTACA T^GTACA
SspI 7051 AATATT AAT^ATT
Van91I 8002 CCACCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 29 CCCGGG C^CCGGG
XmaI 29 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 19 enzymes) #
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BbeI 128 GGCGCC GGCGC^C
6080 GGCGCC
BsmBI 2604 GAGACG CGTCTC(1/5)
5691 GAGACG
BssSI 178 CACGAG CACGAG(-5/-1)
5300 CTCGTG
BstEII 5468 GGTCACC G^GT[ACGT]ACC
5964 GGTCACC
BstPI 5468 GGTCACC G^GT[ACGT]ACC
5964 GGTCACC
EcoO65I 5468 GGTCACC G^GT[ACGT]ACC
5964 GGTCACC
HincII 4753 GTTGAC GT[CT]^[GA]AC
5385 GTCAAC
HindII 4753 GTTGAC GT[CT]^[GA]AC
5385 GTCAAC
HindIII 2115 AAGCTT A^AGCTT
4460 AAGCTT
KasI 128 GGCGCC G^GCGCC
6080 GGCGCC
MslI 1556 CACAGAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
7507 CACTCTGATG
NaeI 6454 GCCGGC GCC^GGC
7057 GCCGGC
NarI 128 GGCGCC GG^CGCC
6080 GGCGCC
NgoMIV 6454 GCCGGC G^CCGGC
7057 GCCGGC
SapI 232 GAAGAGC GCTCTTC(1/4)
1304 GCTCTTC
SexAI 936 ACCAGGT A^CC[AT]GGT
7010 ACCTGGT
SfoI 128 GGCGCC GGC^GCC
6080 GGCGCC
SphI 1296 GCATGC GCATG^C
7475 GCATGC
XcmI 6818 CCAGAACAGCGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
7100 CCAGTGCTAGGATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 141 enzymes) #
#########################################
AatII AccII AccIII AciI AcsI
AcyI AfaI AluI AlwI AlwNI
AocI ApaI ApoI Asp700I AspI
AvaI AvaII BamHI BanI BanII
BbsI BbvCI BbvI BcgI BclI
BcnI BfaI BglI BglII BlpI
BmyI BpmI Bpu1102I BpuAI BsaBI
BsaHI BsaI BsaJI BsaWI BseAI
BseRI BsgI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspEI BspMI
BsrBI BsrFI BsrI BssKI BstF5I
BstNI BstUI BstXI BstYI Bsu36I
Cac8I CelII CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI Eco81I EcoNI EcoO109I EcoRII
EcoT14I FauI FbaI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MamI MboI MboII MflI MnlI
MroI MseI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV NspI PaeR7I PflFI PleI
PpuMI PshAI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
Tth111I TthHB8I XbaI XhoI XhoII
XmnI