Restriction map for
pF1KA0309
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# NO CUT ENZYMES ( 45 enzymes) #
##################################
AccIII Alw44I ApaLI AscI AseI
AsnI BamHI BclI BseAI BsiWI
Bsp1407I BspDI BspEI BsrGI BssHII
Bst1107I BstZ17I ClaI CpoI DrdI
EcoRI EcoT22I FbaI KspI MluI
MroI NdeI NheI NotI NsiI
PacI PmeI PshBI PvuI RsrII
SacII SalI SbfI SfiI SnaBI
Sse8387I SspBI SspI SwaI XbaI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AatII 9023 GACGTC GACGT^C
AccI 4362 GTCTAC GT^[AC][GT]AC
AccII 2332 CGCG CG^CG
AclI 1991 AACGTT AA^CGTT
AflII 5559 CTTAAG C^TTAAG
AflIII 4053 ACATGT A^C[GA][CT]GT
Asp700I 509 GAATTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvrII 6778 CCTAGG C^CTAGG
BbeI 2979 GGCGCC GGCGC^C
BbrPI 3661 CACGTG CAC^GTG
BcgI 8813 CGACCCGGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 5559 CTTAAG C^TTAAG
BglII 7206 AGATCT A^GATCT
BlnI 6778 CCTAGG C^CTAGG
BsaAI 3661 CACGTG [CT]AC^GT[GA]
BsaBI 2731 GATGTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BspLU11I 4053 ACATGT A^CATGT
BsrBI 4946 CCGCTC CCGCTC(-3/-3)
BstBI 7697 TTCGAA TT^CGAA
BstUI 2332 CGCG CG^CG
DraI 2008 TTTAAA TTT^AAA
DraIII 5635 CACCGAGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 4856 CGGCCG C^GGCCG
EclXI 4856 CGGCCG C^GGCCG
Eco52I 4856 CGGCCG C^GGCCG
EcoRV 6214 GATATC GAT^ATC
FseI 6313 GGCCGGCC GGCCGG^CC
HindIII 1837 AAGCTT A^AGCTT
HpaI 3498 GTTAAC GTT^AAC
KasI 2979 GGCGCC G^GCGCC
MamI 2731 GATGTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 5482 CAATTG C^AATTG
MunI 5482 CAATTG C^AATTG
MvnI 2332 CGCG CG^CG
NarI 2979 GGCGCC GG^CGCC
NruI 2331 TCGCGA TCG^CGA
NspV 7697 TTCGAA TT^CGAA
PmaCI 3661 CACGTG CAC^GTG
PmlI 3661 CACGTG CAC^GTG
Psp1406I 1991 AACGTT AA^CGTT
SexAI 4017 ACCTGGT A^CC[AT]GGT
SfoI 2979 GGCGCC GGC^GCC
SfuI 7697 TTCGAA TT^CGAA
SgrAI 6592 CACCGGCG C[GA]^CCGG[CT]G
SpeI 7807 ACTAGT A^CTAGT
XmnI 509 GAATTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 16 enzymes) #
###################################
Acc65I 4398 GGTACC G^GTACC
4629 GGTACC
AcsI 6265 GAATTT [GA]^AATT[CT]
6409 AAATTC
AgeI 2661 ACCGGT A^CCGGT
4395 ACCGGT
Aor51HI 2201 AGCGCT AGC^GCT
6395 AGCGCT
ApoI 6265 GAATTT [GA]^AATT[CT]
6409 AAATTC
Asp718I 4398 GGTACC G^GTACC
4629 GGTACC
BciVI 1173 GGATAC GTATCC(6/5)
4969 GTATCC
BsiEI 2659 CGACCG CG[GA][CT]^CG
4856 CGGCCG
BsmBI 2235 GAGACG CGTCTC(1/5)
7988 GAGACG
BspHI 999 TCATGA T^CATGA
2591 TCATGA
Eco47III 2201 AGCGCT AGC^GCT
6395 AGCGCT
KpnI 4398 GGTACC GGTAC^C
4629 GGTACC
PinAI 2661 ACCGGT A^CCGGT
4395 ACCGGT
RcaI 999 TCATGA T^CATGA
2591 TCATGA
ScaI 656 AGTACT AGT^ACT
1586 AGTACT
SphI 4087 GCATGC GCATG^C
5427 GCATGC
#########################################
# 3 OR MORE CUT ENZYMES ( 151 enzymes) #
#########################################
AciI AcyI AfaI AhdI AluI
AlwI AlwNI AocI ApaI AspEI
AspHI AspI AvaI AvaII AviII
BalI BanI BanII BbsI BbvCI
BbvI BcnI BfaI BglI BlpI
BmyI BpmI Bpu1102I BpuAI BsaHI
BsaI BsaJI BsaWI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI BsoBI Bsp1286I BspMI BsrDI
BsrFI BsrI BssKI BssSI BstAPI
BstEII BstF5I BstNI BstPI BstXI
BstYI Bsu36I Cac8I CelII CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI Eam1105I EarI Eco81I
EcoNI EcoO109I EcoO65I EcoRII EcoT14I
FauI Fnu4HI FokI FspI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HincII HindII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MwoI NaeI NciI
NcoI NdeII NgoMIV NlaIII NlaIV
NspI PaeR7I PflFI PflMI PleI
PpuMI PshAI PstI PvuII RsaI
SacI SapI Sau3AI Sau96I ScrFI
SfaNI SfcI SmaI SmlI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI Tth111I TthHB8I
Van91I XcmI XhoI XhoII XmaCI
XmaI