Restriction map for
pF1KA0294
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# NO CUT ENZYMES ( 68 enzymes) #
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AatII Acc65I AclI AgeI AhdI
ApaI AscI AseI AsnI Asp718I
AspEI AvrII BbrPI BcgI BciVI
BlnI BlpI Bpu1102I BsiWI BsmI
Bsp1407I BsrDI BsrGI BssHII Bst1107I
BstBI BstZ17I CelII CpoI DrdI
Eam1105I EcoRV FseI HpaI KpnI
KspI MfeI MluI MunI NaeI
NdeI NgoMIV NheI NotI NruI
NspV PacI PaeR7I PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
RsrII SacII ScaI SexAI SfiI
SfuI SgrAI SpeI SspBI SspI
StuI SwaI XhoI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AccIII 3175 TCCGGA T^CCGGA
AflII 1243 CTTAAG C^TTAAG
Alw44I 3952 GTGCAC G^TGCAC
Aor51HI 331 AGCGCT AGC^GCT
ApaLI 3952 GTGCAC G^TGCAC
AspI 1053 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 821 TGCGCA TGC^GCA
BbeI 3990 GGCGCC GGCGC^C
BbvCI 334 GCTGAGG CCTCAGC(-5/-2)
BfrI 1243 CTTAAG C^TTAAG
BglII 1081 AGATCT A^GATCT
BsaAI 1231 TACGTA [CT]AC^GT[GA]
BseAI 3175 TCCGGA T^CCGGA
BspDI 3744 ATCGAT AT^CGAT
BspEI 3175 TCCGGA T^CCGGA
BsrFI 3483 GCCGGT [GA]^CCGG[CT]
BstAPI 2792 GCATCCTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 3703 GGTGACC G^GT[ACGT]ACC
BstPI 3703 GGTGACC G^GT[ACGT]ACC
Cfr10I 3483 GCCGGT [GA]^CCGG[CT]
ClaI 3744 ATCGAT AT^CGAT
DraIII 3159 CACGTTGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 2867 CGGCCG C^GGCCG
EclXI 2867 CGGCCG C^GGCCG
Eco47III 331 AGCGCT AGC^GCT
Eco52I 2867 CGGCCG C^GGCCG
EcoO65I 3703 GGTGACC G^GT[ACGT]ACC
EcoRI 2354 GAATTC G^AATTC
EcoT22I 1463 ATGCAT ATGCA^T
FspI 821 TGCGCA TGC^GCA
HindIII 1819 AAGCTT A^AGCTT
KasI 3990 GGCGCC G^GCGCC
MroI 3175 TCCGGA T^CCGGA
NarI 3990 GGCGCC GG^CGCC
NcoI 1451 CCATGG C^CATGG
NsiI 1463 ATGCAT ATGCA^T
PflFI 1053 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 2712 CCATCAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuI 1578 CGATCG CGAT^CG
SacI 1714 GAGCTC GAGCT^C
SalI 1213 GTCGAC G^TCGAC
SbfI 3351 CCTGCAGG CCTGCA^GG
SfoI 3990 GGCGCC GGC^GCC
SmaI 2371 CCCGGG CCC^GGG
SnaBI 1231 TACGTA TAC^GTA
SphI 2801 GCATGC GCATG^C
Sse8387I 3351 CCTGCAGG CCTGCA^GG
Tth111I 1053 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 2712 CCATCAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 3813 TCTAGA T^CTAGA
XmaCI 2371 CCCGGG C^CCGGG
XmaI 2371 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 16 enzymes) #
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AccI 1213 GTCGAC GT^[AC][GT]AC
3140 GTCTAC
AocI 3305 CCTCAGG CC^T[ACGT]AGG
3640 CCTCAGG
BamHI 3082 GGATCC G^GATCC
3858 GGATCC
BclI 1761 TGATCA T^GATCA
3110 TGATCA
BglI 580 GCCCGCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
3907 GCCAGCTCGGC
BsaWI 3175 TCCGGA [AT]^CCGG[AT]
3517 ACCGGA
BsgI 182 CTGCAC GTGCAG(16/14)
3586 CTGCAC
BsmBI 2736 CGTCTC CGTCTC(1/5)
3017 CGTCTC
BspLU11I 1519 ACATGT A^CATGT
2034 ACATGT
BsrBI 26 CCGCTC CCGCTC(-3/-3)
1038 CCGCTC
Bsu36I 3305 CCTCAGG CC^T[ACGT]AGG
3640 CCTCAGG
DraI 864 TTTAAA TTT^AAA
1551 TTTAAA
Eco81I 3305 CCTCAGG CC^T[ACGT]AGG
3640 CCTCAGG
FbaI 1761 TGATCA T^GATCA
3110 TGATCA
PpuMI 811 AGGTCCT [GA]G^G[AT]CC[CT]
3781 GGGTCCC
SapI 772 GAAGAGC GCTCTTC(1/4)
3982 GAAGAGC
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# 3 OR MORE CUT ENZYMES ( 122 enzymes) #
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AccII AciI AcsI AcyI AfaI
AflIII AluI AlwI AlwNI ApoI
Asp700I AspHI AvaI AvaII BalI
BanI BanII BbsI BbvI BcnI
BfaI BmyI BpmI BpuAI BsaBI
BsaHI BsaI BsaJI BseRI BsiEI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspHI BspMI BsrI
BssKI BssSI BstF5I BstNI BstUI
BstXI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HincII HindII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MamI MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV NspI PleI
PstI PvuII RcaI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XcmI
XhoII XmnI