Restriction map for
pF1KA0260
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# NO CUT ENZYMES ( 144 enzymes) #
##################################
Acc65I AccI AccIII AclI AcsI
AflII AflIII AgeI AhdI Alw44I
AlwI AlwNI AocI Aor51HI ApaI
ApaLI ApoI AscI Asp700I Asp718I
AspEI AspI AvaII AviII AvrII
BamHI BanI BbeI BbvCI BcgI
BciVI BclI BcnI BfaI BfrI
BglII BlnI BpmI BsaBI BsaI
BsaWI BseAI BsiWI BsmAI BsmBI
BsmI Bsp1407I BspDI BspEI BspLU11I
BspMI BsrBI BsrGI BsrI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstXI BstYI BstZ17I Bsu36I Cfr13I
ClaI CpoI DraII DrdI DsaI
Eam1105I Eco47III Eco81I EcoNI EcoO109I
EcoO65I EcoRI EcoRV EcoT14I EcoT22I
FbaI FseI FspI HincII HindII
HindIII HpaI KasI KpnI KspI
MaeI MaeIII MamI MfeI MflI
MluI MroI MslI MunI NarI
NciI NcoI NdeI NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PleI PmeI PpuMI
PshAI Psp1406I PvuI RsrII SacII
SalI Sau96I SbfI SexAI SfiI
SfoI SfuI SgrAI SmaI SpeI
Sse8387I SspBI SspI StuI StyI
SwaI Tsp45I Tth111I XbaI XhoI
XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 63 enzymes) #
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AatII 17 GACGTC GACGT^C
AccII 51 CGCG CG^CG
AseI 882 ATTAAT AT^TAAT
AsnI 882 ATTAAT AT^TAAT
AvaI 29 CTCGGG C^[CT]CG[GA]G
BanII 153 GAGCTC G[GA]GC[CT]^C
BbrPI 927 CACGTG CAC^GTG
BbsI 465 GAAGAC GAAGAC(2/6)
BglI 244 GCCACAGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 1008 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1008 GCTGAGC GC^T[ACGT]AGC
BpuAI 465 GAAGAC GAAGAC(2/6)
BsaJI 692 CCCTGG C^C[ACGT][ACGT]GG
BsgI 825 CTGCAC GTGCAG(16/14)
BsiEI 98 CGGCCG CG[GA][CT]^CG
BsmFI 366 GGGAC GGGAC(10/14)
BsoBI 29 CTCGGG C^[CT]CG[GA]G
BspHI 677 TCATGA T^CATGA
BsrFI 136 GCCGGC [GA]^CCGG[CT]
BstAPI 598 GCAAATGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 51 CGCG CG^CG
CelII 1008 GCTGAGC GC^T[ACGT]AGC
CfoI 274 GCGC GCG^C
Cfr10I 136 GCCGGC [GA]^CCGG[CT]
DraI 948 TTTAAA TTT^AAA
DraIII 215 CACTATGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 98 CGGCCG C^GGCCG
EarI 1000 GAAGAG CTCTTC(1/4)
EclXI 98 CGGCCG C^GGCCG
Eco52I 98 CGGCCG C^GGCCG
FauI 49 CCCGC CCCGC(4/6)
HaeII 273 GGCGCT [GA]GCGC^[CT]
HapII 137 CCGG C^CGG
HgaI 94 GCGTC GACGC(5/10)
HhaI 274 GCGC GCG^C
HinP1I 274 GCGC G^CGC
HpaII 137 CCGG C^CGG
Ksp632I 1000 GAAGAG CTCTTC(1/4)
MspA1I 1006 CAGCTG C[AC]G^C[GT]G
MspI 137 CCGG C^CGG
MvnI 51 CGCG CG^CG
NaeI 136 GCCGGC GCC^GGC
NgoMIV 136 GCCGGC G^CCGGC
NspI 24 GCATGC [GA]CATG^[CT]
PflMI 234 CCAGATGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 927 CACGTG CAC^GTG
PmlI 927 CACGTG CAC^GTG
PshBI 882 ATTAAT AT^TAAT
PstI 769 CTGCAG CTGCA^G
PvuII 1006 CAGCTG CAG^CTG
RcaI 677 TCATGA T^CATGA
SacI 153 GAGCTC GAGCT^C
SapI 1000 GAAGAGC GCTCTTC(1/4)
ScaI 819 AGTACT AGT^ACT
SfaNI 718 GATGC GCATC(5/9)
SfcI 769 CTGCAG C^T[GA][CT]AG
SmlI 460 CTCAAG C^T[CT][GA]AG
SnaBI 610 TACGTA TAC^GTA
SphI 24 GCATGC GCATG^C
TaqI 318 TCGA T^CGA
TthHB8I 318 TCGA T^CGA
Van91I 234 CCAGATGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 530 CCAGCTCTGACTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AcyI 17 GACGTC G[GA]^CG[CT]C
93 GGCGTC
AspHI 153 GAGCTC G[AT]GC[AT]^C
187 GTGCTC
BmyI 153 GAGCTC G[AGT]GC[ACT]^C
187 GTGCTC
BsaAI 610 TACGTA [CT]AC^GT[GA]
927 CACGTG
BsaHI 17 GACGTC G[GA]^CG[CT]C
93 GGCGTC
BsiHKAI 153 GAGCTC G[AT]GC[AT]^C
187 GTGCTC
Bsp1286I 153 GAGCTC G[AGT]GC[ACT]^C
187 GTGCTC
BssKI 693 CCTGG ^CC[ACGT]GG
972 CCTGG
BstF5I 426 CATCC GGATG(2/0)
89 GGATG
BstNI 693 CCTGG CC^[AT]GG
972 CCTGG
DpnII 165 GATC ^GATC
550 GATC
EcoRII 693 CCTGG ^CC[AT]GG
972 CCTGG
FokI 426 CATCC GGATG(9/13)
89 GGATG
Hin1I 17 GACGTC G[GA]^CG[CT]C
93 GGCGTC
HinfI 628 GATTC G^A[ACGT]TC
681 GATTC
MboI 165 GATC ^GATC
550 GATC
MvaI 693 CCTGG CC^[AT]GG
972 CCTGG
NdeII 165 GATC ^GATC
550 GATC
NlaIII 25 CATG CATG^
678 CATG
Sau3AI 165 GATC ^GATC
550 GATC
ScrFI 693 CCTGG CC^[ACGT]GG
972 CCTGG
TfiI 628 GATTC G^A[AT]TC
681 GATTC
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# 3 OR MORE CUT ENZYMES ( 29 enzymes) #
#########################################
AciI AfaI AluI BalI BbvI
BseRI BsiYI BslI BsrDI Cac8I
DdeI EaeI Fnu4HI HaeIII HphI
ItaI MaeII MboII MluNI MnlI
MscI MseI MwoI NlaIV RsaI
Tru9I TseI Tsp509I TspRI