##################################
# NO CUT ENZYMES (   53 enzymes) #
##################################

AccIII      AclI        AflII       AgeI        AscI        
AseI        AsnI        AvrII       BamHI       BfrI        
BlnI        BsaBI       BseAI       BsiWI       BsmI        
BspEI       BspHI       Bst1107I    BstBI       BstZ17I     
DraI        DrdI        EcoRI       EcoRV       EcoT22I     
FseI        HindIII     HpaI        MamI        MfeI        
MroI        MseI        MunI        NdeI        NotI        
NsiI        NspV        PacI        PinAI       PmeI        
PshBI       Psp1406I    PvuI        RcaI        SfuI        
SgrAI       SnaBI       SpeI        SphI        SspI        
SwaI        Tru9I       XbaI        


###################################
# ONE CUT ENZYMES (   34 enzymes) #
###################################

Acc65I       5997  GGTACC               G^GTACC
AcsI         2977  AAATTC               [GA]^AATT[CT]
AhdI         5968  GACATCACGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI         6784  CCTGAGG              CC^T[ACGT]AGG
ApoI         2977  AAATTC               [GA]^AATT[CT]
Asp700I      2760  GAAAGGGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I      5997  GGTACC               G^GTACC
AspEI        5968  GACATCACGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BclI         7538  TGATCA               T^GATCA
BsaWI        7527  ACCGGA               [AT]^CCGG[AT]
BsmBI        1226  CGTCTC               CGTCTC(1/5)
BspDI        6622  ATCGAT               AT^CGAT
BspLU11I     1811  ACATGT               A^CATGT
Bsu36I       6784  CCTGAGG              CC^T[ACGT]AGG
ClaI         6622  ATCGAT               AT^CGAT
CpoI         7234  CGGACCG              CG^G[AT]CCG
Eam1105I     5968  GACATCACGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I       6784  CCTGAGG              CC^T[ACGT]AGG
FbaI         7538  TGATCA               T^GATCA
KpnI         5997  GGTACC               GGTAC^C
KspI          749  CCGCGG               CCGC^GG
MluI         2400  ACGCGT               A^CGCGT
NruI          304  TCGCGA               TCG^CGA
PaeR7I       7024  CTCGAG               C^TCGAG
RsrII        7234  CGGACCG              CG^G[AT]CCG
SacII         749  CCGCGG               CCGC^GG
SalI         5908  GTCGAC               G^TCGAC
SbfI         4662  CCTGCAGG             CCTGCA^GG
SfiI         1190  GGCCTGTGCGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
Sse8387I     4662  CCTGCAGG             CCTGCA^GG
TfiI         7370  GATTC                G^A[AT]TC
Tsp509I      2978  AATT                 ^AATT
XhoI         7024  CTCGAG               C^TCGAG
XmnI         2760  GAAAGGGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   13 enzymes) #
###################################

AatII        2389  GACGTC               GACGT^C
             5875  GACGTC              
Alw44I       1153  GTGCAC               G^TGCAC
             4678  GTGCAC              
Aor51HI      4637  AGCGCT               AGC^GCT
             5180  AGCGCT              
ApaLI        1153  GTGCAC               G^TGCAC
             4678  GTGCAC              
BciVI        4067  GTATCC               GTATCC(6/5)
             7157  GTATCC              
BssHII         50  GCGCGC               G^CGCGC
             1277  GCGCGC              
Eco47III     4637  AGCGCT               AGC^GCT
             5180  AGCGCT              
NheI         1062  GCTAGC               G^CTAGC
             3957  GCTAGC              
ScaI         1733  AGTACT               AGT^ACT
             2951  AGTACT              
SmaI         1216  CCCGGG               CCC^GGG
             4882  CCCGGG              
StuI         1880  AGGCCT               AGG^CCT
             6713  AGGCCT              
XmaCI        1216  CCCGGG               C^CCGGG
             4882  CCCGGG              
XmaI         1216  CCCGGG               C^CCGGG
             4882  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (  158 enzymes) #
#########################################

AccI        AccII       AciI        AcyI        AfaI        
AflIII      AluI        AlwI        AlwNI       ApaI        
AspHI       AspI        AvaI        AvaII       AviII       
BalI        BanI        BanII       BbeI        BbrPI       
BbsI        BbvCI       BbvI        BcgI        BcnI        
BfaI        BglI        BglII       BlpI        BmyI        
BpmI        Bpu1102I    BpuAI       BsaAI       BsaHI       
BsaI        BsaJI       BseRI       BsgI        BsiEI       
BsiHKAI     BsiYI       BslI        BsmAI       BsmFI       
BsoBI       Bsp1286I    Bsp1407I    BspMI       BsrBI       
BsrDI       BsrFI       BsrGI       BsrI        BssKI       
BssSI       BstAPI      BstEII      BstF5I      BstNI       
BstPI       BstUI       BstXI       BstYI       Cac8I       
CelII       CfoI        Cfr10I      Cfr13I      DdeI        
DpnII       DraII       DraIII      DsaI        EaeI        
EagI        EarI        EclXI       Eco52I      EcoNI       
EcoO109I    EcoO65I     EcoRII      EcoT14I     FauI        
Fnu4HI      FokI        FspI        HaeII       HaeIII      
HapII       HgaI        HhaI        Hin1I       HinP1I      
HincII      HindII      HinfI       HpaII       HphI        
ItaI        KasI        Ksp632I     MaeI        MaeII       
MaeIII      MboI        MboII       MflI        MluNI       
MnlI        MscI        MslI        MspA1I      MspI        
MvaI        MvnI        MwoI        NaeI        NarI        
NciI        NcoI        NdeII       NgoMIV      NlaIII      
NlaIV       NspI        PflFI       PflMI       PleI        
PmaCI       PmlI        PpuMI       PshAI       PstI        
PvuII       RsaI        SacI        SapI        Sau3AI      
Sau96I      ScrFI       SexAI       SfaNI       SfcI        
SfoI        SmlI        SspBI       StyI        TaqI        
TseI        Tsp45I      TspRI       Tth111I     TthHB8I     
Van91I      XcmI        XhoII