Restriction map for
pF1KA0197
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# NO CUT ENZYMES ( 86 enzymes) #
##################################
AatII AclI AgeI AhdI AocI
Aor51HI AscI AseI AsnI Asp700I
AspEI AspI AviII AvrII BalI
BamHI BbvCI BcgI BciVI BlnI
BsaI BsiEI BsiWI BsmBI BspDI
BsrBI BsrDI BsrFI BssHII BssSI
Bst1107I BstZ17I Bsu36I Cfr10I ClaI
CpoI DraI DraIII EaeI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI FseI FspI HgaI KspI
MluI MluNI MscI NaeI NgoMIV
NotI NruI PacI PaeR7I PflFI
PinAI PmeI PpuMI PshAI PshBI
Psp1406I PvuI RsrII SacI SacII
SalI SapI SbfI SfiI SgrAI
SmaI SnaBI SpeI Sse8387I StuI
SwaI Tth111I XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 51 enzymes) #
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Acc65I 1583 GGTACC G^GTACC
AccI 2760 GTCTAC GT^[AC][GT]AC
AccIII 1797 TCCGGA T^CCGGA
AcyI 118 GGCGCC G[GA]^CG[CT]C
AflII 616 CTTAAG C^TTAAG
AflIII 2899 ACATGT A^C[GA][CT]GT
Alw44I 376 GTGCAC G^TGCAC
ApaI 1115 GGGCCC GGGCC^C
ApaLI 376 GTGCAC G^TGCAC
Asp718I 1583 GGTACC G^GTACC
AvaI 1694 CTCGGG C^[CT]CG[GA]G
BbeI 118 GGCGCC GGCGC^C
BbrPI 3098 CACGTG CAC^GTG
BfrI 616 CTTAAG C^TTAAG
BglI 3858 GCCTAATTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaBI 1227 GATTGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 118 GGCGCC G[GA]^CG[CT]C
BsaWI 1797 TCCGGA [AT]^CCGG[AT]
BseAI 1797 TCCGGA T^CCGGA
BsoBI 1694 CTCGGG C^[CT]CG[GA]G
Bsp1407I 2559 TGTACA T^GTACA
BspEI 1797 TCCGGA T^CCGGA
BspHI 3378 TCATGA T^CATGA
BspLU11I 2899 ACATGT A^CATGT
BspMI 1180 GCAGGT ACCTGC(4/8)
BsrGI 2559 TGTACA T^GTACA
BstEII 724 GGTGACC G^GT[ACGT]ACC
BstPI 724 GGTGACC G^GT[ACGT]ACC
DrdI 3293 GACTTATTCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 724 GGTGACC G^GT[ACGT]ACC
HaeII 118 GGCGCC [GA]GCGC^[CT]
Hin1I 118 GGCGCC G[GA]^CG[CT]C
HpaI 360 GTTAAC GTT^AAC
KasI 118 GGCGCC G^GCGCC
KpnI 1583 GGTACC GGTAC^C
MamI 1227 GATTGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 1797 TCCGGA T^CCGGA
NarI 118 GGCGCC GG^CGCC
NcoI 947 CCATGG C^CATGG
NheI 3696 GCTAGC G^CTAGC
PflMI 3328 CCAGTGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 3098 CACGTG CAC^GTG
PmlI 3098 CACGTG CAC^GTG
RcaI 3378 TCATGA T^CATGA
SexAI 639 ACCTGGT A^CC[AT]GGT
SfoI 118 GGCGCC GGC^GCC
SphI 975 GCATGC GCATG^C
SspBI 2559 TGTACA T^GTACA
Van91I 3328 CCAGTGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 2529 TCTAGA T^CTAGA
XcmI 3304 CCAGAATATGCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 33 enzymes) #
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AccII 52 CGCG CG^CG
141 CGCG
AspHI 376 GTGCAC G[AT]GC[AT]^C
1017 GAGCAC
AvaII 3114 GGACC G^G[AT]CC
3938 GGACC
BanII 11 GAGCCC G[GA]GC[CT]^C
1115 GGGCCC
BbsI 210 GAAGAC GAAGAC(2/6)
1499 GTCTTC
BclI 1242 TGATCA T^GATCA
1832 TGATCA
BcnI 404 CCCGG CC^[GC]GG
3240 CCGGG
BglII 2091 AGATCT A^GATCT
3438 AGATCT
BpuAI 210 GAAGAC GAAGAC(2/6)
1499 GTCTTC
BsaAI 157 TACGTG [CT]AC^GT[GA]
3098 CACGTG
BsiHKAI 376 GTGCAC G[AT]GC[AT]^C
1017 GAGCAC
BsmI 3209 GAATGC GAATGC(1/-1)
3388 GAATGC
BstAPI 684 GCAACGATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1206 GCAGCCTCTGC
BstUI 52 CGCG CG^CG
141 CGCG
DraII 572 AGGCCCT [GA]G^G[ACGT]CC[CT]
1115 GGGCCCT
EcoO109I 572 AGGCCCT [GA]G^G[ACGT]CC[CT]
1115 GGGCCCT
EcoRV 1108 GATATC GAT^ATC
1687 GATATC
EcoT14I 947 CCATGG C^C[AT][AT]GG
3458 CCTTGG
EcoT22I 973 ATGCAT ATGCA^T
3642 ATGCAT
FbaI 1242 TGATCA T^GATCA
1832 TGATCA
HincII 360 GTTAAC GT[CT]^[GA]AC
430 GTTGAC
HindII 360 GTTAAC GT[CT]^[GA]AC
430 GTTGAC
HindIII 1304 AAGCTT A^AGCTT
3601 AAGCTT
MaeII 158 ACGT A^CGT
3099 ACGT
MvnI 52 CGCG CG^CG
141 CGCG
NciI 404 CCCGG CC^[GC]GG
3240 CCGGG
NdeI 1704 CATATG CA^TATG
2945 CATATG
NsiI 973 ATGCAT ATGCA^T
3642 ATGCAT
PvuII 2110 CAGCTG CAG^CTG
3395 CAGCTG
ScaI 2068 AGTACT AGT^ACT
2295 AGTACT
SmlI 616 CTTAAG C^T[CT][GA]AG
3247 CTTGAG
SspI 450 AATATT AAT^ATT
1987 AATATT
StyI 947 CCATGG C^C[AT][AT]GG
3458 CCTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 88 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI BanI BbvI BfaI
BlpI BmyI BpmI Bpu1102I BsaJI
BseRI BsgI BsiYI BslI BsmAI
BsmFI Bsp1286I BsrI BssKI BstBI
BstF5I BstNI BstXI BstYI Cac8I
CelII CfoI Cfr13I DdeI DpnII
DsaI EarI EcoRI EcoRII FauI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeIII MboI MboII
MfeI MflI MnlI MseI MslI
MspA1I MspI MunI MvaI MwoI
NdeII NlaIII NlaIV NspI NspV
PleI PstI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SfuI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII