Restriction map for
pF1KA0187
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# NO CUT ENZYMES ( 76 enzymes) #
##################################
AatII Acc65I AccIII AflII AflIII
AgeI AscI AseI AsnI Asp718I
AviII AvrII BamHI BbeI BbrPI
BfrI BlnI BsaBI BseAI BsiWI
Bsp1407I BspDI BspEI BspLU11I BsrBI
BsrGI BssHII BssSI BstAPI BstEII
BstPI ClaI CpoI EcoO65I FauI
FseI FspI HpaI KasI KpnI
KspI MamI MluI MroI NaeI
NarI NdeI NgoMIV NheI NotI
NruI PacI PaeR7I PinAI PmaCI
PmeI PmlI PshAI PshBI PvuI
RsrII SacII SalI SbfI ScaI
SfiI SfoI SgrAI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
XhoI
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# ONE CUT ENZYMES ( 60 enzymes) #
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AccI 2910 GTATAC GT^[AC][GT]AC
AclI 489 AACGTT AA^CGTT
AcyI 3418 GGCGTC G[GA]^CG[CT]C
Alw44I 2371 GTGCAC G^TGCAC
Aor51HI 974 AGCGCT AGC^GCT
ApaLI 2371 GTGCAC G^TGCAC
AspI 1048 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 3158 TGGCCA TGG^CCA
BcgI 99 CGAAGAAGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 3303 GTATCC GTATCC(6/5)
BclI 1422 TGATCA T^GATCA
BglI 1153 GCCAAGATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 441 AGATCT A^GATCT
BlpI 1532 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1532 GCTCAGC GC^T[ACGT]AGC
BsaAI 2761 TACGTG [CT]AC^GT[GA]
BsaHI 3418 GGCGTC G[GA]^CG[CT]C
BsaWI 830 ACCGGA [AT]^CCGG[AT]
BsiEI 3587 CGGCCG CG[GA][CT]^CG
BsmBI 95 GAGACG CGTCTC(1/5)
BspHI 3606 TCATGA T^CATGA
BspMI 3689 ACCTGC ACCTGC(4/8)
BsrFI 3585 ACCGGC [GA]^CCGG[CT]
Bst1107I 2910 GTATAC GTA^TAC
BstBI 3182 TTCGAA TT^CGAA
BstXI 2717 CCATTATCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 2910 GTATAC GTA^TAC
CelII 1532 GCTCAGC GC^T[ACGT]AGC
Cfr10I 3585 ACCGGC [GA]^CCGG[CT]
DraI 981 TTTAAA TTT^AAA
DraIII 1796 CACTCAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 354 GACGATTGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 3587 CGGCCG C^GGCCG
EclXI 3587 CGGCCG C^GGCCG
Eco47III 974 AGCGCT AGC^GCT
Eco52I 3587 CGGCCG C^GGCCG
EcoRI 3779 GAATTC G^AATTC
EcoRV 805 GATATC GAT^ATC
EcoT22I 2926 ATGCAT ATGCA^T
FbaI 1422 TGATCA T^GATCA
HaeII 974 AGCGCT [GA]GCGC^[CT]
Hin1I 3418 GGCGTC G[GA]^CG[CT]C
MfeI 3838 CAATTG C^AATTG
MluNI 3158 TGGCCA TGG^CCA
MscI 3158 TGGCCA TGG^CCA
MslI 408 CATTAACATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 3838 CAATTG C^AATTG
NcoI 3414 CCATGG C^CATGG
NsiI 2926 ATGCAT ATGCA^T
NspV 3182 TTCGAA TT^CGAA
PflFI 1048 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Psp1406I 489 AACGTT AA^CGTT
RcaI 3606 TCATGA T^CATGA
SexAI 3373 ACCTGGT A^CC[AT]GGT
SfuI 3182 TTCGAA TT^CGAA
SmaI 633 CCCGGG CCC^GGG
Tth111I 1048 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 2124 TCTAGA T^CTAGA
XmaCI 633 CCCGGG C^CCGGG
XmaI 633 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AccII 2587 CGCG CG^CG
3598 CGCG
AhdI 3370 GACACCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3584 GACCGGCCGTC
AlwNI 612 CAGGTTCTG CAG[ACGT][ACGT][ACGT]^CTG
1879 CAGAAACTG
AocI 3462 CCTGAGG CC^T[ACGT]AGG
3759 CCTCAGG
ApaI 1099 GGGCCC GGGCC^C
2949 GGGCCC
Asp700I 2671 GAAAATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3768 GAAGCTCTTC
AspEI 3370 GACACCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3584 GACCGGCCGTC
AvaI 633 CCCGGG C^[CT]CG[GA]G
2813 CCCGAG
BbvCI 2026 GCTGAGG CCTCAGC(-5/-2)
3829 GCTGAGG
BsaI 1909 GAGACC GGTCTC(1/5)
3582 GAGACC
BsmI 1277 GAATGC GAATGC(1/-1)
3383 GAATGC
BsoBI 633 CCCGGG C^[CT]CG[GA]G
2813 CCCGAG
BsrDI 1741 CATTGC GCAATG(2/0)
2929 CATTGC
BstUI 2587 CGCG CG^CG
3598 CGCG
Bsu36I 3462 CCTGAGG CC^T[ACGT]AGG
3759 CCTCAGG
DsaI 3392 CCACGG C^C[GA][CT]GG
3414 CCATGG
EaeI 3158 TGGCCA [CT]^GGCC[GA]
3587 CGGCCG
Eam1105I 3370 GACACCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3584 GACCGGCCGTC
Eco81I 3462 CCTGAGG CC^T[ACGT]AGG
3759 CCTCAGG
EcoNI 167 CCTTTCACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2033 CCTTTCTGAGG
MvnI 2587 CGCG CG^CG
3598 CGCG
NspI 893 ACATGC [GA]CATG^[CT]
2924 ACATGC
PpuMI 264 GGGACCT [GA]G^G[AT]CC[CT]
3755 AGGACCT
PstI 76 CTGCAG CTGCA^G
1592 CTGCAG
StuI 733 AGGCCT AGG^CCT
2030 AGGCCT
XcmI 1103 CCACCCATGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2204 CCAGATTTCTTGTGG
XmnI 2671 GAAAATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3768 GAAGCTCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 95 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AspHI AvaII BanI BanII
BbsI BbvI BcnI BfaI BmyI
BpmI BpuAI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BsrI BssKI BstF5I BstNI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraII EarI EcoO109I EcoRII
EcoT14I Fnu4HI FokI HaeIII HapII
HgaI HhaI HinP1I HincII HindII
HindIII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV PflMI PleI PvuII
RsaI SacI SapI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XhoII