Restriction map for
pF1KA0179
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# NO CUT ENZYMES ( 101 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AflII AgeI AhdI Alw44I
Aor51HI ApaLI AscI AseI AsnI
Asp718I AspEI AspI AviII AvrII
BamHI BanI BbeI BclI BfrI
BglII BlnI BlpI Bpu1102I BsaI
BsaWI BseAI BsiWI BsmI Bsp1407I
BspEI BspHI BsrGI BssHII BssSI
Bst1107I BstUI BstZ17I CelII CpoI
DraI DrdI Eam1105I Eco47III EcoRV
EcoT22I FbaI FseI FspI HindIII
KasI KpnI KspI MfeI MluI
MroI MunI MvnI NarI NcoI
NdeI NheI NotI NruI NsiI
PacI PflFI PinAI PmeI PshAI
PshBI Psp1406I PvuI PvuII RcaI
RsrII SacII SalI SbfI ScaI
SexAI SfiI SfoI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SspI SwaI Tth111I XbaI XmaCI
XmaI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AcyI 48 GGCGTC G[GA]^CG[CT]C
Asp700I 145 GAAGAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI 2162 TGGCCA TGG^CCA
BbrPI 1268 CACGTG CAC^GTG
BcgI 1278 CGAGCCAGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 706 GTATCC GTATCC(6/5)
BsaAI 1268 CACGTG [CT]AC^GT[GA]
BsaBI 505 GATCAGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 48 GGCGTC G[GA]^CG[CT]C
BsiEI 20 CGGCCG CG[GA][CT]^CG
BsmBI 634 GAGACG CGTCTC(1/5)
BspDI 523 ATCGAT AT^CGAT
BspLU11I 1433 ACATGT A^CATGT
BsrBI 77 GAGCGG CCGCTC(-3/-3)
BstAPI 1164 GCACCACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 595 TTCGAA TT^CGAA
BstEII 670 GGTGACC G^GT[ACGT]ACC
BstPI 670 GGTGACC G^GT[ACGT]ACC
BstXI 2201 CCAGGGCTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 26 AGATCC [GA]^GATC[CT]
ClaI 523 ATCGAT AT^CGAT
DraIII 2113 CACGGGGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 20 CGGCCG C^GGCCG
EclXI 20 CGGCCG C^GGCCG
Eco52I 20 CGGCCG C^GGCCG
EcoO65I 670 GGTGACC G^GT[ACGT]ACC
EcoRI 2029 GAATTC G^AATTC
EcoT14I 2188 CCAAGG C^C[AT][AT]GG
HaeII 911 AGCGCC [GA]GCGC^[CT]
Hin1I 48 GGCGTC G[GA]^CG[CT]C
HincII 199 GTTAAC GT[CT]^[GA]AC
HindII 199 GTTAAC GT[CT]^[GA]AC
HpaI 199 GTTAAC GTT^AAC
MamI 505 GATCAGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 26 AGATCC [GA]^GATC[CT]
MluNI 2162 TGGCCA TGG^CCA
MscI 2162 TGGCCA TGG^CCA
MseI 200 TTAA T^TAA
MslI 102 CATCAGCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NspI 1433 ACATGT [GA]CATG^[CT]
NspV 595 TTCGAA TT^CGAA
PaeR7I 2078 CTCGAG C^TCGAG
PmaCI 1268 CACGTG CAC^GTG
PmlI 1268 CACGTG CAC^GTG
SfuI 595 TTCGAA TT^CGAA
StuI 1241 AGGCCT AGG^CCT
StyI 2188 CCAAGG C^C[AT][AT]GG
Tru9I 200 TTAA T^TAA
XcmI 2147 CCAGCTCACCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 2078 CTCGAG C^TCGAG
XhoII 26 AGATCC [GA]^GATC[CT]
XmnI 145 GAAGAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AcsI 934 AAATTC [GA]^AATT[CT]
2029 GAATTC
AfaI 99 GTAC GT^AC
571 GTAC
AflIII 1269 ACGTGT A^C[GA][CT]GT
1433 ACATGT
AlwI 27 GATCC GGATC(4/5)
526 GATCC
AocI 318 CCTGAGG CC^T[ACGT]AGG
1465 CCTCAGG
ApoI 934 AAATTC [GA]^AATT[CT]
2029 GAATTC
BbvCI 682 GCTGAGG CCTCAGC(-5/-2)
1851 GCTGAGG
BfaI 187 CTAG C^TAG
1475 CTAG
BseRI 685 GAGGAG GAGGAG(10/8)
1312 GAGGAG
BsgI 1199 GTGCAG GTGCAG(16/14)
1286 GTGCAG
BspMI 1169 ACCTGC ACCTGC(4/8)
2142 ACCTGC
BsrDI 177 CATTGC GCAATG(2/0)
1577 CATTGC
BsrFI 18 GCCGGC [GA]^CCGG[CT]
1563 GCCGGC
Bsu36I 318 CCTGAGG CC^T[ACGT]AGG
1465 CCTCAGG
Cfr10I 18 GCCGGC [GA]^CCGG[CT]
1563 GCCGGC
Eco81I 318 CCTGAGG CC^T[ACGT]AGG
1465 CCTCAGG
EcoNI 390 CCTGATGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1594 CCTCCCACAGG
FauI 7 CCCGC CCCGC(4/6)
1606 CCCGC
HgaI 114 GACGC GACGC(5/10)
49 GCGTC
MaeI 187 CTAG C^TAG
1475 CTAG
MaeII 1269 ACGT A^CGT
1297 ACGT
MaeIII 671 GTGAC ^GT[ACGT]AC
1996 GTCAC
MspA1I 40 CAGCGG C[AC]G^C[GT]G
209 CAGCGG
NaeI 18 GCCGGC GCC^GGC
1563 GCCGGC
NgoMIV 18 GCCGGC G^CCGGC
1563 GCCGGC
PstI 1171 CTGCAG CTGCA^G
1654 CTGCAG
RsaI 99 GTAC GT^AC
571 GTAC
SacI 163 GAGCTC GAGCT^C
1687 GAGCTC
SfaNI 65 GCATC GCATC(5/9)
1329 GCATC
Tsp45I 671 GTGAC ^GT[GC]AC
1996 GTCAC
#########################################
# 3 OR MORE CUT ENZYMES ( 75 enzymes) #
#########################################
AciI AluI AlwNI ApaI AspHI
AvaI AvaII BanII BbsI BbvI
BcnI BglI BmyI BpmI BpuAI
BsaJI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BsrI BssKI
BstF5I BstNI Cac8I CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI EcoO109I EcoRII Fnu4HI FokI
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MboI
MboII MnlI MspI MvaI MwoI
NciI NdeII NlaIII NlaIV PflMI
PleI PpuMI SapI Sau3AI Sau96I
ScrFI SfcI SmlI TaqI TfiI
TseI Tsp509I TspRI TthHB8I Van91I