Restriction map for
pF1KA0068
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# NO CUT ENZYMES ( 59 enzymes) #
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AatII AclI AflII AscI AseI
AsnI AspI AviII AvrII BfrI
BlnI BsiEI BsiWI BspHI Bst1107I
BstBI BstZ17I CpoI DraI DrdI
EagI EclXI Eco52I EcoRV FseI
FspI HincII HindII HpaI KspI
MfeI MluI MunI NdeI NotI
NruI NspV PacI PaeR7I PflFI
PmeI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacII SalI SfiI
SfuI SgrAI SpeI SphI SspI
SwaI Tth111I XbaI XhoI
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# ONE CUT ENZYMES ( 56 enzymes) #
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Acc65I 2441 GGTACC G^GTACC
AccI 3409 GTCTAC GT^[AC][GT]AC
AccIII 3299 TCCGGA T^CCGGA
AgeI 2585 ACCGGT A^CCGGT
Aor51HI 1291 AGCGCT AGC^GCT
ApaI 1756 GGGCCC GGGCC^C
Asp718I 2441 GGTACC G^GTACC
BalI 641 TGGCCA TGG^CCA
BbeI 422 GGCGCC GGCGC^C
BbrPI 1219 CACGTG CAC^GTG
BbvCI 3205 CCTCAGC CCTCAGC(-5/-2)
BciVI 2912 GTATCC GTATCC(6/5)
BglI 1482 GCCGCTGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 2873 AGATCT A^GATCT
BlpI 2433 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 2433 GCTGAGC GC^T[ACGT]AGC
BsaBI 1047 GATCCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 1960 GAGACC GGTCTC(1/5)
BseAI 3299 TCCGGA T^CCGGA
BspDI 1974 ATCGAT AT^CGAT
BspEI 3299 TCCGGA T^CCGGA
BspLU11I 497 ACATGT A^CATGT
BstAPI 1185 GCAGGGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1467 GGTGACC G^GT[ACGT]ACC
BstPI 1467 GGTGACC G^GT[ACGT]ACC
CelII 2433 GCTGAGC GC^T[ACGT]AGC
ClaI 1974 ATCGAT AT^CGAT
DraIII 2488 CACAACGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 641 TGGCCA [CT]^GGCC[GA]
Eco47III 1291 AGCGCT AGC^GCT
EcoO65I 1467 GGTGACC G^GT[ACGT]ACC
EcoRI 3620 GAATTC G^AATTC
HindIII 1243 AAGCTT A^AGCTT
KasI 422 GGCGCC G^GCGCC
KpnI 2441 GGTACC GGTAC^C
MamI 1047 GATCCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 641 TGGCCA TGG^CCA
MroI 3299 TCCGGA T^CCGGA
MscI 641 TGGCCA TGG^CCA
NaeI 2753 GCCGGC GCC^GGC
NarI 422 GGCGCC GG^CGCC
NcoI 1892 CCATGG C^CATGG
NgoMIV 2753 GCCGGC G^CCGGC
NheI 2115 GCTAGC G^CTAGC
PflMI 1950 CCACATCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 2585 ACCGGT A^CCGGT
PmaCI 1219 CACGTG CAC^GTG
PmlI 1219 CACGTG CAC^GTG
SexAI 2038 ACCAGGT A^CC[AT]GGT
SfoI 422 GGCGCC GGC^GCC
SmaI 269 CCCGGG CCC^GGG
SnaBI 584 TACGTA TAC^GTA
StuI 1377 AGGCCT AGG^CCT
Van91I 1950 CCACATCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 269 CCCGGG C^CCGGG
XmaI 269 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 34 enzymes) #
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AflIII 497 ACATGT A^C[GA][CT]GT
2492 ACGTGT
AhdI 25 GACGCGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
451 GACTTCGTGTC
Alw44I 999 GTGCAC G^TGCAC
1249 GTGCAC
AocI 349 CCTGAGG CC^T[ACGT]AGG
1473 CCTTAGG
ApaLI 999 GTGCAC G^TGCAC
1249 GTGCAC
Asp700I 447 GAAGGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1777 GAAAAATTTC
AspEI 25 GACGCGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
451 GACTTCGTGTC
AvaI 269 CCCGGG C^[CT]CG[GA]G
1122 CTCGGG
BanI 422 GGCGCC G^G[CT][GA]CC
2441 GGTACC
BsmI 2188 GAATGC GAATGC(1/-1)
3416 GCATTC
BsoBI 269 CCCGGG C^[CT]CG[GA]G
1122 CTCGGG
Bsp1407I 248 TGTACA T^GTACA
2009 TGTACA
BsrBI 572 CCGCTC CCGCTC(-3/-3)
1620 GAGCGG
BsrDI 1707 CATTGC GCAATG(2/0)
3344 GCAATG
BsrGI 248 TGTACA T^GTACA
2009 TGTACA
BssHII 1090 GCGCGC G^CGCGC
1214 GCGCGC
BssSI 2893 CACGAG CACGAG(-5/-1)
3433 CACGAG
BstXI 632 CCATGTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1760 CCACCATATTGG
Bsu36I 349 CCTGAGG CC^T[ACGT]AGG
1473 CCTTAGG
Eam1105I 25 GACGCGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
451 GACTTCGTGTC
Eco81I 349 CCTGAGG CC^T[ACGT]AGG
1473 CCTTAGG
EcoT22I 149 ATGCAT ATGCA^T
3355 ATGCAT
MseI 3551 TTAA T^TAA
3587 TTAA
NsiI 149 ATGCAT ATGCA^T
3355 ATGCAT
SacI 706 GAGCTC GAGCT^C
2536 GAGCTC
SapI 703 GAAGAGC GCTCTTC(1/4)
1167 GCTCTTC
SbfI 1380 CCTGCAGG CCTGCA^GG
1530 CCTGCAGG
ScaI 890 AGTACT AGT^ACT
1265 AGTACT
SmlI 1737 CTTGAG C^T[CT][GA]AG
1750 CTTGAG
Sse8387I 1380 CCTGCAGG CCTGCA^GG
1530 CCTGCAGG
SspBI 248 TGTACA T^GTACA
2009 TGTACA
Tru9I 3551 TTAA T^TAA
3587 TTAA
XcmI 2381 CCATAGTTGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3558 CCAGAAACATGATGG
XmnI 447 GAAGGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1777 GAAAAATTTC
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# 3 OR MORE CUT ENZYMES ( 109 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI ApoI AspHI
AvaII BamHI BanII BbsI BbvI
BcgI BclI BcnI BfaI BmyI
BpmI BpuAI BsaAI BsaHI BsaJI
BsaWI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmBI BsmFI Bsp1286I
BspMI BsrFI BsrI BssKI BstF5I
BstNI BstUI BstYI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EarI EcoNI EcoO109I EcoRII
EcoT14I FauI FbaI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV NspI
PleI PpuMI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
StyI TaqI TfiI TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII