Restriction map for
pF1KA0055
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# NO CUT ENZYMES ( 109 enzymes) #
##################################
AatII Acc65I AccII AflII AgeI
AhdI AocI ApaI AscI AseI
AsnI Asp718I AspEI AspI AvaI
AvrII BalI BamHI BanI BbeI
BbrPI BcgI BcnI BfrI BglI
BlnI BsiEI BsiWI BsmBI BsoBI
BspDI BsrFI BssHII BstBI BstEII
BstPI BstUI BstXI Bsu36I Cfr10I
ClaI CpoI DrdI DsaI EaeI
EagI Eam1105I EclXI Eco52I Eco81I
EcoNI EcoO65I EcoT14I EcoT22I FseI
HindIII KasI KpnI KspI MfeI
MluI MluNI MscI MunI MvnI
NaeI NarI NciI NcoI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
PvuI PvuII RsrII SacII SalI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I StyI SwaI Tth111I
XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AccI 930 GTATAC GT^[AC][GT]AC
AclI 3035 AACGTT AA^CGTT
Alw44I 109 GTGCAC G^TGCAC
AlwI 1937 GGATC GGATC(4/5)
Aor51HI 2141 AGCGCT AGC^GCT
ApaLI 109 GTGCAC G^TGCAC
AviII 869 TGCGCA TGC^GCA
BanII 2966 GAGCTC G[GA]GC[CT]^C
BbsI 66 GAAGAC GAAGAC(2/6)
BbvCI 1619 CCTCAGC CCTCAGC(-5/-2)
BlpI 2110 GCTGAGC GC^T[ACGT]AGC
BpmI 1136 CTCCAG CTGGAG(16/14)
Bpu1102I 2110 GCTGAGC GC^T[ACGT]AGC
BpuAI 66 GAAGAC GAAGAC(2/6)
BsaAI 196 TACGTG [CT]AC^GT[GA]
BsaBI 2991 GATAGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaJI 2168 CCCAGG C^C[ACGT][ACGT]GG
BsgI 2060 CTGCAC GTGCAG(16/14)
Bsp1407I 2884 TGTACA T^GTACA
BsrBI 2113 GAGCGG CCGCTC(-3/-3)
BsrGI 2884 TGTACA T^GTACA
BssSI 3332 CACGAG CACGAG(-5/-1)
Bst1107I 930 GTATAC GTA^TAC
BstZ17I 930 GTATAC GTA^TAC
CelII 2110 GCTGAGC GC^T[ACGT]AGC
DraII 172 AGGGCCT [GA]G^G[ACGT]CC[CT]
DraIII 1916 CACGAAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 2141 AGCGCT AGC^GCT
EcoO109I 172 AGGGCCT [GA]G^G[ACGT]CC[CT]
EcoRI 1835 GAATTC G^AATTC
EcoRV 3268 GATATC GAT^ATC
FauI 2115 GCGGG CCCGC(4/6)
FspI 869 TGCGCA TGC^GCA
HaeII 2141 AGCGCT [GA]GCGC^[CT]
HpaI 1368 GTTAAC GTT^AAC
MamI 2991 GATAGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MspA1I 394 CAGCGG C[AC]G^C[GT]G
NdeI 2029 CATATG CA^TATG
Psp1406I 3035 AACGTT AA^CGTT
SacI 2966 GAGCTC GAGCT^C
SspBI 2884 TGTACA T^GTACA
SspI 2370 AATATT AAT^ATT
StuI 2840 AGGCCT AGG^CCT
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# TWO CUT ENZYMES ( 37 enzymes) #
###################################
AccIII 353 TCCGGA T^CCGGA
821 TCCGGA
AciI 396 GCGG CCGC(-3/-1)
2115 GCGG
AcyI 965 GACGCC G[GA]^CG[CT]C
1050 GACGCC
AflIII 2239 ACATGT A^C[GA][CT]GT
2812 ACATGT
AlwNI 1148 CAGTTGCTG CAG[ACGT][ACGT][ACGT]^CTG
1400 CAGAAACTG
Asp700I 1021 GAATCAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2611 GAATTGCTTC
BciVI 1863 GGATAC GTATCC(6/5)
2587 GGATAC
BclI 2694 TGATCA T^GATCA
3252 TGATCA
BsaHI 965 GACGCC G[GA]^CG[CT]C
1050 GACGCC
BsaI 526 GAGACC GGTCTC(1/5)
2635 GGTCTC
BseAI 353 TCCGGA T^CCGGA
821 TCCGGA
BseRI 1301 CTCCTC GAGGAG(10/8)
2151 CTCCTC
BsmFI 1116 GGGAC GGGAC(10/14)
3327 GGGAC
BsmI 145 GAATGC GAATGC(1/-1)
617 GAATGC
BspEI 353 TCCGGA T^CCGGA
821 TCCGGA
BspHI 2498 TCATGA T^CATGA
3255 TCATGA
BspLU11I 2239 ACATGT A^CATGT
2812 ACATGT
Cac8I 3 GCCTGC GC[ACGT]^[ACGT]GC
1799 GCAAGC
CfoI 870 GCGC GCG^C
2142 GCGC
DraI 2548 TTTAAA TTT^AAA
2710 TTTAAA
FbaI 2694 TGATCA T^GATCA
3252 TGATCA
HgaI 965 GACGC GACGC(5/10)
1050 GACGC
HhaI 870 GCGC GCG^C
2142 GCGC
Hin1I 965 GACGCC G[GA]^CG[CT]C
1050 GACGCC
HinP1I 870 GCGC G^CGC
2142 GCGC
HincII 1368 GTTAAC GT[CT]^[GA]AC
1749 GTCAAC
HindII 1368 GTTAAC GT[CT]^[GA]AC
1749 GTCAAC
MaeII 197 ACGT A^CGT
3036 ACGT
MroI 353 TCCGGA T^CCGGA
821 TCCGGA
PflMI 1825 CCAGAAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1981 CCAATCACTGG
PstI 2717 CTGCAG CTGCA^G
2952 CTGCAG
RcaI 2498 TCATGA T^CATGA
3255 TCATGA
SapI 1255 GAAGAGC GCTCTTC(1/4)
1408 GCTCTTC
SmlI 364 CTTGAG C^T[CT][GA]AG
2262 CTCAAG
Van91I 1825 CCAGAAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1981 CCAATCACTGG
XcmI 258 CCATTCAATACTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
492 CCAAAAGAGCAATGG
XmnI 1021 GAATCAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2611 GAATTGCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 69 enzymes) #
#########################################
AcsI AfaI AluI ApoI AspHI
AvaII BbvI BfaI BglII BmyI
BsaWI BsiHKAI BsiYI BslI BsmAI
Bsp1286I BspMI BsrDI BsrI BssKI
BstAPI BstF5I BstNI BstYI Cfr13I
DdeI DpnII EarI EcoRII Fnu4HI
FokI HaeIII HapII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeIII
MboI MboII MflI MnlI MseI
MslI MspI MvaI MwoI NdeII
NlaIII NlaIV NspI PleI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII