Restriction map for
pF1KA0010
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# NO CUT ENZYMES ( 91 enzymes) #
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AatII AccI AcyI AflII AgeI
AhdI Aor51HI AscI AspEI AspI
AvrII BalI BbeI BbrPI BbvCI
BfrI BglI BlnI BsaAI BsaBI
BsaHI BsiEI BsiWI BsmBI Bsp1407I
BspDI BspHI BsrFI BsrGI BssHII
Bst1107I BstAPI BstEII BstPI BstZ17I
Cfr10I ClaI CpoI EaeI EagI
Eam1105I EclXI Eco47III Eco52I EcoO65I
FseI Hin1I HpaI KasI KspI
MamI MfeI MluI MluNI MscI
MunI NaeI NarI NdeI NgoMIV
NotI NruI PflFI PinAI PmaCI
PmeI PmlI PshAI PvuI PvuII
RcaI RsrII SacI SacII SalI
SbfI ScaI SfiI SfoI SgrAI
SmaI SnaBI SpeI Sse8387I SspBI
StuI SwaI Tth111I XbaI XmaCI
XmaI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AccIII 756 TCCGGA T^CCGGA
AclI 335 AACGTT AA^CGTT
AflIII 1944 ACATGT A^C[GA][CT]GT
AocI 2590 CCTGAGG CC^T[ACGT]AGG
ApaI 992 GGGCCC GGGCC^C
Asp700I 2389 GAAGGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII 861 TGCGCA TGC^GCA
BamHI 2252 GGATCC G^GATCC
BcgI 1648 CGAGATGCATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 3087 GTATCC GTATCC(6/5)
BglII 2099 AGATCT A^GATCT
BlpI 3243 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 3243 GCTGAGC GC^T[ACGT]AGC
BsaI 626 GGTCTC GGTCTC(1/5)
BseAI 756 TCCGGA T^CCGGA
BsgI 1300 GTGCAG GTGCAG(16/14)
BspEI 756 TCCGGA T^CCGGA
BspLU11I 1944 ACATGT A^CATGT
BsrBI 3127 GAGCGG CCGCTC(-3/-3)
BssSI 1848 CACGAG CACGAG(-5/-1)
BstBI 10 TTCGAA TT^CGAA
BstXI 1870 CCAAACCACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 2590 CCTGAGG CC^T[ACGT]AGG
CelII 3243 GCTGAGC GC^T[ACGT]AGC
DraI 2328 TTTAAA TTT^AAA
DraIII 2761 CACTTGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 2722 GACATCCCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 2590 CCTGAGG CC^T[ACGT]AGG
EcoRI 1552 GAATTC G^AATTC
EcoRV 1443 GATATC GAT^ATC
FspI 861 TGCGCA TGC^GCA
HgaI 27 GACGC GACGC(5/10)
HincII 1398 GTCAAC GT[CT]^[GA]AC
HindII 1398 GTCAAC GT[CT]^[GA]AC
HindIII 652 AAGCTT A^AGCTT
MroI 756 TCCGGA T^CCGGA
NheI 562 GCTAGC G^CTAGC
NspV 10 TTCGAA TT^CGAA
PacI 361 TTAATTAA TTAAT^TAA
PpuMI 2921 AGGACCT [GA]G^G[AT]CC[CT]
Psp1406I 335 AACGTT AA^CGTT
SexAI 1229 ACCTGGT A^CC[AT]GGT
SfuI 10 TTCGAA TT^CGAA
SphI 1654 GCATGC GCATG^C
XmnI 2389 GAAGGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 31 enzymes) #
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Acc65I 1317 GGTACC G^GTACC
1425 GGTACC
Alw44I 2260 GTGCAC G^TGCAC
2897 GTGCAC
AlwNI 298 CAGCTTCTG CAG[ACGT][ACGT][ACGT]^CTG
3144 CAGCACCTG
ApaLI 2260 GTGCAC G^TGCAC
2897 GTGCAC
AseI 2167 ATTAAT AT^TAAT
2886 ATTAAT
AsnI 2167 ATTAAT AT^TAAT
2886 ATTAAT
Asp718I 1317 GGTACC G^GTACC
1425 GGTACC
AvaII 2149 GGTCC G^G[AT]CC
2922 GGACC
BanI 1317 GGTACC G^G[CT][GA]CC
1425 GGTACC
BanII 883 GAGCCC G[GA]GC[CT]^C
992 GGGCCC
BclI 1409 TGATCA T^GATCA
2865 TGATCA
BpmI 2222 CTCCAG CTGGAG(16/14)
2988 CTGGAG
BsaWI 756 TCCGGA [AT]^CCGG[AT]
2744 ACCGGA
BsrDI 261 CATTGC GCAATG(2/0)
731 CATTGC
DsaI 1271 CCATGG C^C[GA][CT]GG
2277 CCATGG
EarI 1618 GAAGAG CTCTTC(1/4)
2619 GAAGAG
EcoNI 996 CCTCTCTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2679 CCTGGGAGAGG
EcoT22I 1652 ATGCAT ATGCA^T
1767 ATGCAT
FbaI 1409 TGATCA T^GATCA
2865 TGATCA
KpnI 1317 GGTACC GGTAC^C
1425 GGTACC
Ksp632I 1618 GAAGAG CTCTTC(1/4)
2619 GAAGAG
NcoI 1271 CCATGG C^CATGG
2277 CCATGG
NsiI 1652 ATGCAT ATGCA^T
1767 ATGCAT
PaeR7I 686 CTCGAG C^TCGAG
2845 CTCGAG
PflMI 2277 CCATGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2760 CCACTTGGTGG
PshBI 2167 ATTAAT AT^TAAT
2886 ATTAAT
SapI 1618 GAAGAGC GCTCTTC(1/4)
2619 GAAGAGC
SspI 194 AATATT AAT^ATT
671 AATATT
Van91I 2277 CCATGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2760 CCACTTGGTGG
XcmI 1991 CCACCCTGGACGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2549 CCAGTGCCGACGTGG
XhoI 686 CTCGAG C^TCGAG
2845 CTCGAG
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# 3 OR MORE CUT ENZYMES ( 91 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI ApoI AspHI AvaI BbsI
BbvI BcnI BfaI BmyI BpuAI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI BsoBI Bsp1286I
BspMI BsrI BssKI BstF5I BstNI
BstUI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraII EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV NspI PleI PstI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII