Restriction map for
pF1KSDF0400
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# NO CUT ENZYMES ( 102 enzymes) #
##################################
AatII Acc65I AccI AflII AflIII
AhdI Alw44I AlwI AocI Aor51HI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AviII AvrII
BamHI BbrPI BcgI BciVI BclI
BfrI BglII BlnI BsaAI BsaBI
BsaI BsiWI BsmI BspDI BspHI
BspLU11I BsrDI BssHII BssSI Bst1107I
BstBI BstYI BstZ17I Bsu36I ClaI
CpoI DraI DrdI Eam1105I Eco47III
Eco81I EcoRV EcoT22I FbaI FseI
FspI HpaI KpnI MamI MfeI
MflI MluI MseI MunI NcoI
NdeI NheI NotI NsiI NspV
PacI PaeR7I PflFI PmaCI PmeI
PmlI PshAI PshBI PvuI RcaI
RsrII SacI SalI SapI SbfI
SfuI SmlI SnaBI SpeI SphI
Sse8387I SspI StuI SwaI TaqI
Tru9I Tth111I TthHB8I XbaI XhoI
XhoII XmnI
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# ONE CUT ENZYMES ( 35 enzymes) #
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AccIII 1883 TCCGGA T^CCGGA
AclI 1234 AACGTT AA^CGTT
AcsI 314 GAATTC [GA]^AATT[CT]
AgeI 1361 ACCGGT A^CCGGT
ApoI 314 GAATTC [GA]^AATT[CT]
AspHI 1609 GTGCTC G[AT]GC[AT]^C
BbvCI 618 CCTCAGC CCTCAGC(-5/-2)
BfaI 1606 CTAG C^TAG
BseAI 1883 TCCGGA T^CCGGA
BsiEI 2068 CGGCCG CG[GA][CT]^CG
BsiHKAI 1609 GTGCTC G[AT]GC[AT]^C
BspEI 1883 TCCGGA T^CCGGA
BstAPI 174 GCAGACCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 453 CCAGTGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraIII 1921 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 2068 CGGCCG C^GGCCG
EclXI 2068 CGGCCG C^GGCCG
Eco52I 2068 CGGCCG C^GGCCG
EcoRI 314 GAATTC G^AATTC
MaeI 1606 CTAG C^TAG
MroI 1883 TCCGGA T^CCGGA
NaeI 203 GCCGGC GCC^GGC
NgoMIV 203 GCCGGC G^CCGGC
NruI 1939 TCGCGA TCG^CGA
NspI 513 GCATGT [GA]CATG^[CT]
PinAI 1361 ACCGGT A^CCGGT
Psp1406I 1234 AACGTT AA^CGTT
PstI 1634 CTGCAG CTGCA^G
ScaI 965 AGTACT AGT^ACT
SexAI 1922 ACCTGGT A^CC[AT]GGT
SfcI 1634 CTGCAG C^T[GA][CT]AG
SfiI 867 GGCCCAGACGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SgrAI 202 CGCCGGCG C[GA]^CCGG[CT]G
TfiI 2150 GATTC G^A[AT]TC
Tsp509I 315 AATT ^AATT
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# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AlwNI 244 CAGCGGCTG CAG[ACGT][ACGT][ACGT]^CTG
1009 CAGTACCTG
BalI 1145 TGGCCA TGG^CCA
1758 TGGCCA
BbeI 224 GGCGCC GGCGC^C
717 GGCGCC
BlpI 363 GCTGAGC GC^T[ACGT]AGC
1818 GCTCAGC
Bpu1102I 363 GCTGAGC GC^T[ACGT]AGC
1818 GCTCAGC
BsaWI 1361 ACCGGT [AT]^CCGG[AT]
1883 TCCGGA
BsgI 172 GTGCAG GTGCAG(16/14)
766 GTGCAG
BsmBI 1167 CGTCTC CGTCTC(1/5)
1842 CGTCTC
Bsp1407I 593 TGTACA T^GTACA
1280 TGTACA
BsrBI 28 GAGCGG CCGCTC(-3/-3)
79 GAGCGG
BsrGI 593 TGTACA T^GTACA
1280 TGTACA
BstEII 1332 GGTCACC G^GT[ACGT]ACC
1449 GGTCACC
BstPI 1332 GGTCACC G^GT[ACGT]ACC
1449 GGTCACC
CelII 363 GCTGAGC GC^T[ACGT]AGC
1818 GCTCAGC
EcoO65I 1332 GGTCACC G^GT[ACGT]ACC
1449 GGTCACC
HindIII 384 AAGCTT A^AGCTT
2000 AAGCTT
KasI 224 GGCGCC G^GCGCC
717 GGCGCC
MaeII 908 ACGT A^CGT
1235 ACGT
MluNI 1145 TGGCCA TGG^CCA
1758 TGGCCA
MscI 1145 TGGCCA TGG^CCA
1758 TGGCCA
MslI 759 CATCCGGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1452 CACCTGGATG
NarI 224 GGCGCC GG^CGCC
717 GGCGCC
PpuMI 1050 GGGTCCC [GA]G^G[AT]CC[CT]
1931 AGGACCT
SfoI 224 GGCGCC GGC^GCC
717 GGCGCC
SspBI 593 TGTACA T^GTACA
1280 TGTACA
XcmI 471 CCAGTCCCCGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1415 CCAAGAATGTGCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 95 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
ApaI AvaI AvaII BanI BanII
BbsI BbvI BcnI BglI BmyI
BpmI BpuAI BsaHI BsaJI BseRI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrFI BsrI BssKI
BstF5I BstNI BstUI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EarI EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HincII HindII HinfI
HpaII HphI ItaI Ksp632I KspI
MaeIII MboI MboII MnlI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV PflMI PleI
PvuII RsaI SacII Sau3AI Sau96I
ScrFI SfaNI SmaI StyI TseI
Tsp45I TspRI Van91I XmaCI XmaI