Restriction map for
pF1KSDF0187
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# NO CUT ENZYMES ( 126 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcyI AflII AgeI Aor51HI AscI
AseI AsnI Asp718I AspI AviII
AvrII BalI BbeI BbrPI BbvCI
BcgI BciVI BclI BcnI BfrI
BglI BglII BlnI BlpI Bpu1102I
BsaAI BsaBI BsaHI BsaWI BseAI
BsiEI BsiWI BsmI Bsp1407I BspDI
BspEI BspMI BsrBI BsrFI BsrGI
BssHII Bst1107I BstAPI BstBI BstEII
BstPI BstXI BstZ17I CelII Cfr10I
ClaI CpoI DraI DrdI EagI
EclXI Eco47III Eco52I EcoO65I EcoRV
FbaI FseI FspI HapII HgaI
Hin1I HpaI HpaII KasI KpnI
KspI MamI MfeI MluI MluNI
MroI MscI MspI MunI NaeI
NarI NciI NcoI NdeI NgoMIV
NheI NotI NruI NspV PacI
PflFI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI PvuII
RsrII SacII SalI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI Tth111I XmaCI
XmaI
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# ONE CUT ENZYMES ( 34 enzymes) #
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AfaI 1638 GTAC GT^AC
AflIII 107 ACATGT A^C[GA][CT]GT
AhdI 419 GACAACAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1554 GTGCAC G^TGCAC
AocI 955 CCTGAGG CC^T[ACGT]AGG
ApaI 205 GGGCCC GGGCC^C
ApaLI 1554 GTGCAC G^TGCAC
AspEI 419 GACAACAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BanI 471 GGTGCC G^G[CT][GA]CC
BsaI 629 GAGACC GGTCTC(1/5)
BsmBI 548 CGTCTC CGTCTC(1/5)
BspLU11I 107 ACATGT A^CATGT
BsrDI 673 GCAATG GCAATG(2/0)
Bsu36I 955 CCTGAGG CC^T[ACGT]AGG
DraIII 1557 CACTAAGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 256 CGGCCA [CT]^GGCC[GA]
Eam1105I 419 GACAACAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 955 CCTGAGG CC^T[ACGT]AGG
EcoNI 321 CCTGCCCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 1658 ATGCAT ATGCA^T
HaeII 179 GGCGCT [GA]GCGC^[CT]
HincII 828 GTCAAC GT[CT]^[GA]AC
HindII 828 GTCAAC GT[CT]^[GA]AC
MaeII 912 ACGT A^CGT
MseI 1247 TTAA T^TAA
MspA1I 659 CAGCGG C[AC]G^C[GT]G
NsiI 1658 ATGCAT ATGCA^T
NspI 107 ACATGT [GA]CATG^[CT]
PpuMI 425 AGGTCCC [GA]G^G[AT]CC[CT]
PstI 346 CTGCAG CTGCA^G
RsaI 1638 GTAC GT^AC
SapI 580 GCTCTTC GCTCTTC(1/4)
Tru9I 1247 TTAA T^TAA
XbaI 390 TCTAGA T^CTAGA
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# TWO CUT ENZYMES ( 29 enzymes) #
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AccII 868 CGCG CG^CG
1668 CGCG
AcsI 1442 GAATTC [GA]^AATT[CT]
1526 GAATTC
ApoI 1442 GAATTC [GA]^AATT[CT]
1526 GAATTC
BamHI 480 GGATCC G^GATCC
1274 GGATCC
BbsI 100 GAAGAC GAAGAC(2/6)
394 GAAGAC
BfaI 301 CTAG C^TAG
391 CTAG
BpuAI 100 GAAGAC GAAGAC(2/6)
394 GAAGAC
BspHI 612 TCATGA T^CATGA
1296 TCATGA
BstUI 868 CGCG CG^CG
1668 CGCG
Cac8I 456 GCTCGC GC[ACGT]^[ACGT]GC
993 GCCAGC
DraII 205 GGGCCCC [GA]G^G[ACGT]CC[CT]
425 AGGTCCC
DsaI 26 CCACGG C^C[GA][CT]GG
1112 CCACGG
EarI 581 CTCTTC CTCTTC(1/4)
85 GAAGAG
EcoO109I 205 GGGCCCC [GA]G^G[ACGT]CC[CT]
425 AGGTCCC
EcoRI 1442 GAATTC G^AATTC
1526 GAATTC
HindIII 1068 AAGCTT A^AGCTT
1487 AAGCTT
Ksp632I 581 CTCTTC CTCTTC(1/4)
85 GAAGAG
MaeI 301 CTAG C^TAG
391 CTAG
MslI 693 CATGGCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1129 CACAGATGTG
MvnI 868 CGCG CG^CG
1668 CGCG
PaeR7I 498 CTCGAG C^TCGAG
1535 CTCGAG
PflMI 633 CCAGGCGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
669 CCAGGCAATGG
PleI 493 GAGTC GAGTC(4/5)
826 GAGTC
RcaI 612 TCATGA T^CATGA
1296 TCATGA
SmlI 498 CTCGAG C^T[CT][GA]AG
1535 CTCGAG
TspRI 247 CAGTG CA[GC]TG[ACGT][ACGT]^
1197 CACTG
Van91I 633 CCAGGCGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
669 CCAGGCAATGG
XcmI 192 CCAGAACACTTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
891 CCAGAAAGAGTTTGG
XhoI 498 CTCGAG C^TCGAG
1535 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 69 enzymes) #
#########################################
AciI AluI AlwI AlwNI Asp700I
AspHI AvaI AvaII BanII BbvI
BmyI BpmI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BsrI BssKI BssSI
BstF5I BstNI BstYI CfoI Cfr13I
DdeI DpnII EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HhaI HinP1I
HinfI HphI ItaI MaeIII MboI
MboII MflI MnlI MvaI MwoI
NdeII NlaIII NlaIV SacI Sau3AI
Sau96I ScrFI SfaNI SfcI StuI
StyI TaqI TfiI TseI Tsp45I
Tsp509I TthHB8I XhoII XmnI