Restriction map for
pF1KSDF0183
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# NO CUT ENZYMES ( 134 enzymes) #
##################################
Acc65I AccI AccIII AclI AcsI
AflII AflIII AgeI AhdI Alw44I
AlwNI Aor51HI ApaI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AviII AvrII BamHI
BbrPI BbsI BbvCI BcgI BclI
BfaI BfrI BglI BglII BlnI
BlpI BpmI Bpu1102I BpuAI BsaAI
BsaBI BsaWI BseAI BseRI BsgI
BsiWI BsmBI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrDI BsrGI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstXI BstZ17I CelII ClaI
CpoI DraIII DrdI Eam1105I Eco47III
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI HincII HindII HindIII
HinfI HpaI KpnI MaeI MamI
MfeI MluI MroI MunI NdeI
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PleI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SalI SapI SbfI ScaI SfiI
SfuI SgrAI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI TfiI Tsp45I TspRI Tth111I
XbaI XcmI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 52 enzymes) #
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AatII 427 GACGTC GACGT^C
AocI 86 CCTCAGG CC^T[ACGT]AGG
AvaI 66 CCCGGG C^[CT]CG[GA]G
AvaII 645 GGTCC G^G[AT]CC
BalI 290 TGGCCA TGG^CCA
BanI 61 GGCGCC G^G[CT][GA]CC
BanII 247 GAGCTC G[GA]GC[CT]^C
BbeI 61 GGCGCC GGCGC^C
BciVI 266 GTATCC GTATCC(6/5)
BsaI 220 GAGACC GGTCTC(1/5)
BsoBI 66 CCCGGG C^[CT]CG[GA]G
BsrBI 198 GAGCGG CCGCTC(-3/-3)
BsrI 492 ACTGG ACTGG(1/-1)
BstAPI 96 GCAGGGGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 70 GGATCT [GA]^GATC[CT]
Bsu36I 86 CCTCAGG CC^T[ACGT]AGG
DraI 513 TTTAAA TTT^AAA
DraII 407 AGGCCCC [GA]G^G[ACGT]CC[CT]
EarI 403 GAAGAG CTCTTC(1/4)
Eco81I 86 CCTCAGG CC^T[ACGT]AGG
EcoNI 279 CCTGCCGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 407 AGGCCCC [GA]G^G[ACGT]CC[CT]
FauI 46 CCCGC CCCGC(4/6)
HaeII 61 GGCGCC [GA]GCGC^[CT]
KasI 61 GGCGCC G^GCGCC
Ksp632I 403 GAAGAG CTCTTC(1/4)
KspI 47 CCGCGG CCGC^GG
MaeII 428 ACGT A^CGT
MaeIII 632 GTAAC ^GT[ACGT]AC
MboII 403 GAAGA GAAGA(8/7)
MflI 70 GGATCT [GA]^GATC[CT]
MluNI 290 TGGCCA TGG^CCA
MscI 290 TGGCCA TGG^CCA
MslI 432 CATCAATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 58 GCCGGC GCC^GGC
NarI 61 GGCGCC GG^CGCC
NgoMIV 58 GCCGGC G^CCGGC
NspI 363 GCATGT [GA]CATG^[CT]
PflMI 636 CCAACACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 29 CTGCAG CTGCA^G
PvuII 457 CAGCTG CAG^CTG
SacI 247 GAGCTC GAGCT^C
SacII 47 CCGCGG CCGC^GG
SexAI 641 ACCTGGT A^CC[AT]GGT
SfaNI 582 GCATC GCATC(5/9)
SfcI 29 CTGCAG C^T[GA][CT]AG
SfoI 61 GGCGCC GGC^GCC
SmaI 66 CCCGGG CCC^GGG
Van91I 636 CCAACACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 70 GGATCT [GA]^GATC[CT]
XmaCI 66 CCCGGG C^CCGGG
XmaI 66 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 36 enzymes) #
###################################
AccII 48 CGCG CG^CG
232 CGCG
AcyI 61 GGCGCC G[GA]^CG[CT]C
427 GACGTC
AfaI 123 GTAC GT^AC
606 GTAC
AlwI 70 GGATC GGATC(4/5)
495 GGATC
AspHI 133 GTGCTC G[AT]GC[AT]^C
247 GAGCTC
BmyI 133 GTGCTC G[AGT]GC[ACT]^C
247 GAGCTC
BsaHI 61 GGCGCC G[GA]^CG[CT]C
427 GACGTC
BsiEI 56 CGGCCG CG[GA][CT]^CG
260 CGGCCG
BsiHKAI 133 GTGCTC G[AT]GC[AT]^C
247 GAGCTC
BsmAI 220 GAGAC GTCTC(1/5)
464 GAGAC
BsmFI 113 GGGAC GGGAC(10/14)
425 GGGAC
Bsp1286I 133 GTGCTC G[AGT]GC[ACT]^C
247 GAGCTC
BspMI 285 GCAGGT ACCTGC(4/8)
306 GCAGGT
BsrFI 58 GCCGGC [GA]^CCGG[CT]
262 GCCGGT
BstF5I 252 CATCC GGATG(2/0)
583 CATCC
BstUI 48 CGCG CG^CG
232 CGCG
CfoI 62 GCGC GCG^C
80 GCGC
Cfr10I 58 GCCGGC [GA]^CCGG[CT]
262 GCCGGT
Cfr13I 408 GGCCC G^G[ACGT]CC
645 GGTCC
EagI 56 CGGCCG C^GGCCG
260 CGGCCG
EclXI 56 CGGCCG C^GGCCG
260 CGGCCG
Eco52I 56 CGGCCG C^GGCCG
260 CGGCCG
FokI 252 CATCC GGATG(9/13)
583 CATCC
HgaI 416 GACGC GACGC(5/10)
569 GCGTC
HhaI 62 GCGC GCG^C
80 GCGC
Hin1I 61 GGCGCC G[GA]^CG[CT]C
427 GACGTC
HinP1I 62 GCGC G^CGC
80 GCGC
HphI 140 TCACC GGTGA(8/7)
444 TCACC
MseI 509 TTAA T^TAA
514 TTAA
MvnI 48 CGCG CG^CG
232 CGCG
NcoI 293 CCATGG C^CATGG
356 CCATGG
NlaIV 61 GGCGCC GG[ACGT]^[ACGT]CC
408 GGCCCC
RsaI 123 GTAC GT^AC
606 GTAC
Sau96I 408 GGCCC G^G[ACGT]CC
645 GGTCC
Tru9I 509 TTAA T^TAA
514 TTAA
Tsp509I 511 AATT ^AATT
525 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 36 enzymes) #
#########################################
AciI AluI BbvI BcnI BsaJI
BsiYI BslI BssKI BstNI Cac8I
DdeI DpnII DsaI EaeI EcoRII
EcoT14I Fnu4HI HaeIII HapII HpaII
ItaI MboI MnlI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
Sau3AI ScrFI StyI TaqI TseI
TthHB8I