Restriction map for
pF1KB5641
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# NO CUT ENZYMES ( 94 enzymes) #
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Acc65I AccIII AflII AgeI AocI
ApaI AscI Asp718I AviII AvrII
BbeI BciVI BfrI BglI BlnI
BsaBI BsaI BseAI BsiEI BsiWI
Bsp1407I BspDI BspEI BspHI BspMI
BsrBI BsrFI BsrGI BssHII Bst1107I
BstAPI BstBI BstEII BstPI BstZ17I
Bsu36I Cfr10I ClaI CpoI DraIII
EagI EclXI Eco52I Eco81I EcoNI
EcoO65I EcoRI FauI FseI FspI
HgaI HindIII KasI KpnI KspI
MamI MfeI MluI MroI MunI
NaeI NarI NdeI NgoMIV NheI
NotI NruI NspV PacI PinAI
PmeI PvuI RcaI RsrII SacI
SacII SalI SapI SbfI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI Sse8387I SspBI SspI
SwaI XbaI XmaCI XmaI
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# ONE CUT ENZYMES ( 59 enzymes) #
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AccI 1801 GTAGAC GT^[AC][GT]AC
AclI 789 AACGTT AA^CGTT
AcsI 1565 GAATTT [GA]^AATT[CT]
AhdI 1873 GACTGGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 734 GTGCAC G^TGCAC
ApaLI 734 GTGCAC G^TGCAC
ApoI 1565 GAATTT [GA]^AATT[CT]
AseI 1087 ATTAAT AT^TAAT
AsnI 1087 ATTAAT AT^TAAT
Asp700I 7 GAAGTACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 1873 GACTGGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 734 GTGCAC G[AT]GC[AT]^C
AspI 1378 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 435 CTCGAG C^[CT]CG[GA]G
BalI 53 TGGCCA TGG^CCA
BanI 759 GGCACC G^G[CT][GA]CC
BbrPI 1774 CACGTG CAC^GTG
BbsI 655 GAAGAC GAAGAC(2/6)
BbvCI 509 CCTCAGC CCTCAGC(-5/-2)
BcgI 2064 CGAGCCTAATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 849 TGATCA T^GATCA
BlpI 1437 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1437 GCTCAGC GC^T[ACGT]AGC
BpuAI 655 GAAGAC GAAGAC(2/6)
BsaAI 1774 CACGTG [CT]AC^GT[GA]
BsaWI 743 ACCGGA [AT]^CCGG[AT]
BseRI 772 GAGGAG GAGGAG(10/8)
BsiHKAI 734 GTGCAC G[AT]GC[AT]^C
BsmBI 963 CGTCTC CGTCTC(1/5)
BsmI 1662 GAATGC GAATGC(1/-1)
BsoBI 435 CTCGAG C^[CT]CG[GA]G
BspLU11I 1409 ACATGT A^CATGT
BssSI 1627 CACGAG CACGAG(-5/-1)
CelII 1437 GCTCAGC GC^T[ACGT]AGC
DraI 1143 TTTAAA TTT^AAA
Eam1105I 1873 GACTGGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRV 2117 GATATC GAT^ATC
EcoT22I 1175 ATGCAT ATGCA^T
FbaI 849 TGATCA T^GATCA
HpaI 1126 GTTAAC GTT^AAC
MluNI 53 TGGCCA TGG^CCA
MscI 53 TGGCCA TGG^CCA
NsiI 1175 ATGCAT ATGCA^T
PaeR7I 435 CTCGAG C^TCGAG
PflFI 1378 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 750 CCAGAACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 1774 CACGTG CAC^GTG
PmlI 1774 CACGTG CAC^GTG
PshAI 638 GACCCCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 1087 ATTAAT AT^TAAT
Psp1406I 789 AACGTT AA^CGTT
SmlI 435 CTCGAG C^T[CT][GA]AG
SphI 1113 GCATGC GCATG^C
StuI 587 AGGCCT AGG^CCT
Tth111I 1378 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 750 CCAGAACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 284 CCAACATCAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 435 CTCGAG C^TCGAG
XmnI 7 GAAGTACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 24 enzymes) #
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AatII 73 GACGTC GACGT^C
1996 GACGTC
AccII 1498 CGCG CG^CG
1516 CGCG
AcyI 73 GACGTC G[GA]^CG[CT]C
1996 GACGTC
AflIII 1409 ACATGT A^C[GA][CT]GT
2081 ACGTGT
Aor51HI 1671 AGCGCT AGC^GCT
2036 AGCGCT
BamHI 246 GGATCC G^GATCC
746 GGATCC
BanII 366 GAGCCC G[GA]GC[CT]^C
415 GAGCCC
BfaI 523 CTAG C^TAG
992 CTAG
BpmI 982 CTCCAG CTGGAG(16/14)
1423 CTGGAG
BsaHI 73 GACGTC G[GA]^CG[CT]C
1996 GACGTC
BsrDI 906 CATTGC GCAATG(2/0)
1611 CATTGC
BstUI 1498 CGCG CG^CG
1516 CGCG
BstXI 1640 CCAAGATTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1667 CCAAAGCGCTGG
CfoI 1672 GCGC GCG^C
2037 GCGC
DrdI 786 GACAACGTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1804 GACAAACCAGTC
Eco47III 1671 AGCGCT AGC^GCT
2036 AGCGCT
HaeII 1671 AGCGCT [GA]GCGC^[CT]
2036 AGCGCT
HhaI 1672 GCGC GCG^C
2037 GCGC
Hin1I 73 GACGTC G[GA]^CG[CT]C
1996 GACGTC
HinP1I 1672 GCGC G^CGC
2037 GCGC
MaeI 523 CTAG C^TAG
992 CTAG
MvnI 1498 CGCG CG^CG
1516 CGCG
ScaI 9 AGTACT AGT^ACT
1781 AGTACT
Tsp509I 487 AATT ^AATT
1566 AATT
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# 3 OR MORE CUT ENZYMES ( 81 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AvaII BbvI BcnI BglII BmyI
BsaJI BsgI BsiYI BslI BsmAI
BsmFI Bsp1286I BsrI BssKI BstF5I
BstNI BstYI Cac8I Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
EcoO109I EcoRII EcoT14I Fnu4HI FokI
HaeIII HapII HincII HindII HinfI
HpaII HphI ItaI Ksp632I MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MwoI NciI NcoI NdeII NlaIII
NlaIV NspI PleI PpuMI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI StyI TaqI TfiI
Tru9I TseI Tsp45I TspRI TthHB8I
XhoII