Restriction map for
pF1KB5479
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# NO CUT ENZYMES ( 128 enzymes) #
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AatII AccI AccIII AclI AcsI
AcyI AflII AflIII AgeI AhdI
AocI Aor51HI ApaI ApoI AscI
AseI AsnI Asp700I AspEI AspI
AvrII BamHI BbeI BbrPI BbvCI
BcgI BclI BfrI BlnI BlpI
Bpu1102I BsaAI BsaBI BsaHI BsaI
BseAI BsiEI BsiWI BsmAI BsmBI
Bsp1407I BspDI BspEI BspLU11I BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstZ17I Bsu36I
CelII Cfr10I ClaI CpoI DraI
DrdI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI HgaI Hin1I
HindIII HpaI KasI KspI MaeII
MamI MfeI MluI MroI MseI
MunI NaeI NarI NgoMIV NheI
NotI NruI NsiI NspV PacI
PflFI PinAI PmaCI PmeI PmlI
PshBI Psp1406I PvuI RsrII SacII
SalI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
SwaI Tru9I Tsp509I Tth111I XbaI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 35 enzymes) #
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AccII 1105 CGCG CG^CG
Alw44I 586 GTGCAC G^TGCAC
ApaLI 586 GTGCAC G^TGCAC
AvaI 1259 CTCGAG C^[CT]CG[GA]G
AviII 1159 TGCGCA TGC^GCA
BalI 23 TGGCCA TGG^CCA
BanII 612 GAGCTC G[GA]GC[CT]^C
BciVI 437 GTATCC GTATCC(6/5)
BcnI 406 CCGGG CC^[GC]GG
BglI 1056 GCCATTGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 746 AGATCT A^GATCT
BsmI 969 GAATGC GAATGC(1/-1)
BsoBI 1259 CTCGAG C^[CT]CG[GA]G
BspHI 1010 TCATGA T^CATGA
BsrBI 363 CCGCTC CCGCTC(-3/-3)
BsrDI 714 CATTGC GCAATG(2/0)
BstUI 1105 CGCG CG^CG
DraIII 159 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoNI 519 CCTGAAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FspI 1159 TGCGCA TGC^GCA
HaeII 170 AGCGCC [GA]GCGC^[CT]
HincII 37 GTCAAC GT[CT]^[GA]AC
HindII 37 GTCAAC GT[CT]^[GA]AC
MluNI 23 TGGCCA TGG^CCA
MscI 23 TGGCCA TGG^CCA
MvnI 1105 CGCG CG^CG
NciI 406 CCGGG CC^[GC]GG
NdeI 1450 CATATG CA^TATG
PaeR7I 1259 CTCGAG C^TCGAG
PvuII 445 CAGCTG CAG^CTG
RcaI 1010 TCATGA T^CATGA
SacI 612 GAGCTC GAGCT^C
SapI 411 GAAGAGC GCTCTTC(1/4)
StuI 1268 AGGCCT AGG^CCT
XhoI 1259 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 25 enzymes) #
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Acc65I 384 GGTACC G^GTACC
943 GGTACC
AlwNI 442 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
577 CAGCCTCTG
Asp718I 384 GGTACC G^GTACC
943 GGTACC
AspHI 586 GTGCAC G[AT]GC[AT]^C
612 GAGCTC
BbsI 814 GAAGAC GAAGAC(2/6)
1405 GTCTTC
BfaI 187 CTAG C^TAG
541 CTAG
BpuAI 814 GAAGAC GAAGAC(2/6)
1405 GTCTTC
BsaWI 284 ACCGGA [AT]^CCGG[AT]
965 ACCGGA
BseRI 1048 GAGGAG GAGGAG(10/8)
1051 GAGGAG
BsiHKAI 586 GTGCAC G[AT]GC[AT]^C
612 GAGCTC
BspMI 818 ACCTGC ACCTGC(4/8)
124 GCAGGT
EaeI 23 TGGCCA [CT]^GGCC[GA]
1101 TGGCCG
EarI 391 GAAGAG CTCTTC(1/4)
411 GAAGAG
KpnI 384 GGTACC GGTAC^C
943 GGTACC
Ksp632I 391 GAAGAG CTCTTC(1/4)
411 GAAGAG
MaeI 187 CTAG C^TAG
541 CTAG
MspA1I 150 CAGCGG C[AC]G^C[GT]G
445 CAGCTG
NcoI 911 CCATGG C^CATGG
1319 CCATGG
PleI 1003 GACTC GAGTC(4/5)
1193 GACTC
PshAI 64 GACCACAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
556 GACCACAGTC
PstI 122 CTGCAG CTGCA^G
1253 CTGCAG
SfcI 122 CTGCAG C^T[GA][CT]AG
1253 CTGCAG
TaqI 1260 TCGA T^CGA
1392 TCGA
TthHB8I 1260 TCGA T^CGA
1392 TCGA
XcmI 471 CCAGCCTCCAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
478 CCAGATGGTGCCTGG
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# 3 OR MORE CUT ENZYMES ( 70 enzymes) #
#########################################
AciI AfaI AluI AlwI AvaII
BanI BbvI BmyI BpmI BsaJI
BsgI BsiYI BslI BsmFI Bsp1286I
BsrI BssKI BstF5I BstNI BstXI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI MaeIII MboI MboII
MflI MnlI MslI MspI MvaI
MwoI NdeII NlaIII NlaIV NspI
PflMI PpuMI RsaI Sau3AI Sau96I
ScrFI SfaNI SmlI StyI TfiI
TseI Tsp45I TspRI Van91I XhoII