Restriction map for
pF1KSDB2508
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# NO CUT ENZYMES ( 145 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AgeI AhdI AocI
ApaI ApoI AscI AseI AsnI
Asp718I AspEI AspI AviII AvrII
BbrPI BbsI BbvCI BciVI BclI
BfaI BfrI BglII BlnI BlpI
Bpu1102I BpuAI BsaAI BsaBI BsaWI
BseAI BsgI BsiEI BsiWI BsmBI
BsmFI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrDI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstXI BstZ17I Bsu36I
CelII ClaI CpoI DraI DraII
DraIII DrdI EagI Eam1105I EclXI
Eco52I Eco81I EcoNI EcoO109I EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
FspI HgaI HincII HindII HindIII
HpaI KpnI KspI MaeI MamI
MfeI MluI MroI MseI MslI
MspA1I MunI NdeI NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PinAI PleI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI PvuII RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI SfiI SfuI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI TaqI Tru9I Tsp509I
Tth111I TthHB8I XbaI XcmI XhoI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AccII 185 CGCG CG^CG
AflIII 209 ACGTGT A^C[GA][CT]GT
Aor51HI 61 AGCGCT AGC^GCT
Asp700I 240 GAAAGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 51 CCCGGG C^[CT]CG[GA]G
AvaII 231 GGACC G^G[AT]CC
BalI 566 TGGCCA TGG^CCA
BamHI 496 GGATCC G^GATCC
BcgI 134 GCACTGCCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BpmI 622 CTGGAG CTGGAG(16/14)
BsaI 201 GGTCTC GGTCTC(1/5)
BsoBI 51 CCCGGG C^[CT]CG[GA]G
BspMI 233 ACCTGC ACCTGC(4/8)
BsrFI 38 GCCGGC [GA]^CCGG[CT]
BsrI 684 CCAGT ACTGG(1/-1)
BstUI 185 CGCG CG^CG
BstYI 496 GGATCC [GA]^GATC[CT]
Cfr10I 38 GCCGGC [GA]^CCGG[CT]
DdeI 633 CTCAG C^T[ACGT]AG
EaeI 566 TGGCCA [CT]^GGCC[GA]
Eco47III 61 AGCGCT AGC^GCT
EcoT14I 492 CCATGG C^C[AT][AT]GG
HinfI 255 GAATC G^A[ACGT]TC
HphI 512 TCACC GGTGA(8/7)
MaeII 209 ACGT A^CGT
MaeIII 511 GTCAC ^GT[ACGT]AC
MflI 496 GGATCC [GA]^GATC[CT]
MluNI 566 TGGCCA TGG^CCA
MscI 566 TGGCCA TGG^CCA
MvnI 185 CGCG CG^CG
NaeI 38 GCCGGC GCC^GGC
NcoI 492 CCATGG C^CATGG
NgoMIV 38 GCCGGC G^CCGGC
PflMI 189 CCAGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 412 CTGCAG CTGCA^G
SexAI 533 ACCTGGT A^CC[AT]GGT
SfcI 412 CTGCAG C^T[GA][CT]AG
SgrAI 37 CGCCGGCG C[GA]^CCGG[CT]G
SmaI 51 CCCGGG CCC^GGG
StyI 492 CCATGG C^C[AT][AT]GG
TfiI 255 GAATC G^A[AT]TC
Tsp45I 511 GTCAC ^GT[GC]AC
Van91I 189 CCAGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 496 GGATCC [GA]^GATC[CT]
XmaCI 51 CCCGGG C^CCGGG
XmaI 51 CCCGGG C^CCGGG
XmnI 240 GAAAGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AcyI 35 GGCGCC G[GA]^CG[CT]C
109 GGCGCC
AfaI 3 GTAC GT^AC
489 GTAC
Alw44I 132 GTGCAC G^TGCAC
223 GTGCAC
AlwI 497 GATCC GGATC(4/5)
496 GGATC
ApaLI 132 GTGCAC G^TGCAC
223 GTGCAC
AspHI 132 GTGCAC G[AT]GC[AT]^C
223 GTGCAC
BbeI 35 GGCGCC GGCGC^C
109 GGCGCC
BglI 27 GCCCGGCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
50 GCCCGGGAGGC
BsaHI 35 GGCGCC G[GA]^CG[CT]C
109 GGCGCC
BseRI 45 GAGGAG GAGGAG(10/8)
610 GAGGAG
BsiHKAI 132 GTGCAC G[AT]GC[AT]^C
223 GTGCAC
BsmAI 202 GTCTC GTCTC(1/5)
320 GTCTC
DpnII 372 GATC ^GATC
497 GATC
EarI 607 GAAGAG CTCTTC(1/4)
643 GAAGAG
Hin1I 35 GGCGCC G[GA]^CG[CT]C
109 GGCGCC
KasI 35 GGCGCC G^GCGCC
109 GGCGCC
Ksp632I 607 GAAGAG CTCTTC(1/4)
643 GAAGAG
MboI 372 GATC ^GATC
497 GATC
NarI 35 GGCGCC GG^CGCC
109 GGCGCC
NdeII 372 GATC ^GATC
497 GATC
NlaIII 252 CATG CATG^
493 CATG
RsaI 3 GTAC GT^AC
489 GTAC
Sau3AI 372 GATC ^GATC
497 GATC
SfaNI 308 GCATC GCATC(5/9)
357 GCATC
SfoI 35 GGCGCC GGC^GCC
109 GGCGCC
#########################################
# 3 OR MORE CUT ENZYMES ( 41 enzymes) #
#########################################
AciI AluI AlwNI BanI BanII
BbvI BcnI BmyI BsaJI BsiYI
BslI Bsp1286I BssKI BstF5I BstNI
Cac8I CfoI Cfr13I DsaI EcoRII
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI HinP1I HpaII ItaI
MboII MnlI MspI MvaI MwoI
NciI NlaIV Sau96I ScrFI TseI
TspRI