Restriction map for
pF1KSDB1496
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# NO CUT ENZYMES ( 166 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AflII AflIII
AgeI AhdI Alw44I AocI Aor51HI
ApaLI ApoI AscI AseI AsnI
Asp718I AspEI AspHI AspI AvaI
AviII AvrII BamHI BbeI BbrPI
BbsI BcgI BciVI BclI BcnI
BfrI BglI BlnI BlpI BpmI
Bpu1102I BpuAI BsaAI BsaBI BsaHI
BsaWI BseAI BseRI BsgI BsiEI
BsiHKAI BsiWI BsmBI BsmFI BsoBI
BspDI BspEI BspLU11I BsrDI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstXI BstZ17I Bsu36I
CelII ClaI CpoI DraI DraIII
DrdI EagI Eam1105I EarI EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
FspI HaeII HgaI Hin1I HincII
HindII HindIII HinfI HpaI KasI
KpnI Ksp632I KspI MaeII MamI
MfeI MluI MroI MunI MvnI
NaeI NarI NciI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PleI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspI StuI SwaI
TaqI TfiI Tsp45I Tth111I TthHB8I
Van91I XbaI XcmI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AfaI 522 GTAC GT^AC
AlwI 192 GATCC GGATC(4/5)
AlwNI 594 CAGTCTCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp700I 636 GAATGCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaII 542 GGACC G^G[AT]CC
BalI 59 TGGCCA TGG^CCA
BanI 820 GGCACC G^G[CT][GA]CC
BbvCI 262 GCTGAGG CCTCAGC(-5/-2)
BfaI 436 CTAG C^TAG
BglII 296 AGATCT A^GATCT
BsaI 142 GAGACC GGTCTC(1/5)
BsmI 636 GAATGC GAATGC(1/-1)
Bsp1407I 521 TGTACA T^GTACA
BspHI 770 TCATGA T^CATGA
BspMI 547 ACCTGC ACCTGC(4/8)
BsrBI 406 GAGCGG CCGCTC(-3/-3)
BsrFI 280 ACCGGC [GA]^CCGG[CT]
BsrGI 521 TGTACA T^GTACA
BstF5I 442 CATCC GGATG(2/0)
BstYI 296 AGATCT [GA]^GATC[CT]
Cfr10I 280 ACCGGC [GA]^CCGG[CT]
DsaI 631 CCATGG C^C[GA][CT]GG
EaeI 59 TGGCCA [CT]^GGCC[GA]
EcoT14I 631 CCATGG C^C[AT][AT]GG
FauI 408 GCGGG CCCGC(4/6)
FokI 442 CATCC GGATG(9/13)
HapII 281 CCGG C^CGG
HpaII 281 CCGG C^CGG
MaeI 436 CTAG C^TAG
MaeIII 349 GTAAC ^GT[ACGT]AC
MflI 296 AGATCT [GA]^GATC[CT]
MluNI 59 TGGCCA TGG^CCA
MscI 59 TGGCCA TGG^CCA
MseI 803 TTAA T^TAA
MspA1I 689 CAGCTG C[AC]G^C[GT]G
MspI 281 CCGG C^CGG
NcoI 631 CCATGG C^CATGG
PstI 740 CTGCAG CTGCA^G
PvuII 689 CAGCTG CAG^CTG
RcaI 770 TCATGA T^CATGA
RsaI 522 GTAC GT^AC
SfcI 740 CTGCAG C^T[GA][CT]AG
SgrAI 279 CACCGGCG C[GA]^CCGG[CT]G
SspBI 521 TGTACA T^GTACA
StyI 631 CCATGG C^C[AT][AT]GG
Tru9I 803 TTAA T^TAA
Tsp509I 138 AATT ^AATT
XhoII 296 AGATCT [GA]^GATC[CT]
XmnI 636 GAATGCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 18 enzymes) #
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AciI 326 GCGG CCGC(-3/-1)
408 GCGG
ApaI 38 GGGCCC GGGCC^C
248 GGGCCC
BanII 38 GGGCCC G[GA]GC[CT]^C
248 GGGCCC
BmyI 38 GGGCCC G[AGT]GC[ACT]^C
248 GGGCCC
BsmAI 142 GAGAC GTCTC(1/5)
596 GTCTC
Bsp1286I 38 GGGCCC G[AGT]GC[ACT]^C
248 GGGCCC
Cac8I 258 GCTGGC GC[ACGT]^[ACGT]GC
502 GCCTGC
CfoI 284 GCGC GCG^C
341 GCGC
DpnII 192 GATC ^GATC
297 GATC
HhaI 284 GCGC GCG^C
341 GCGC
HinP1I 284 GCGC G^CGC
341 GCGC
HphI 464 TCACC GGTGA(8/7)
764 TCACC
MboI 192 GATC ^GATC
297 GATC
MboII 233 TCTTC GAAGA(8/7)
236 TCTTC
MslI 183 CATCAATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
765 CACCTTCATG
NdeII 192 GATC ^GATC
297 GATC
Sau3AI 192 GATC ^GATC
297 GATC
SfaNI 607 GATGC GCATC(5/9)
461 GCATC
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# 3 OR MORE CUT ENZYMES ( 25 enzymes) #
#########################################
AluI BbvI BsaJI BsiYI BslI
BsrI BssKI BstNI Cfr13I DdeI
DraII EcoO109I EcoRII Fnu4HI HaeIII
ItaI MnlI MvaI MwoI NlaIII
NlaIV Sau96I ScrFI TseI TspRI