Restriction map for
pF1KSDB1483
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# NO CUT ENZYMES ( 76 enzymes) #
##################################
Acc65I AclI AflII AgeI AhdI
AocI Aor51HI ApaI AscI AseI
AsnI Asp718I AspEI AspI AvrII
BciVI BfaI BfrI BlnI BlpI
Bpu1102I BspDI BspLU11I BssHII Bst1107I
BstBI BstZ17I Bsu36I CelII ClaI
DraI DraIII DrdI Eam1105I Eco47III
Eco81I EcoRV EcoT22I FseI HindIII
HpaI KpnI KspI MaeI MfeI
MluI MunI NdeI NheI NotI
NruI NsiI NspV PacI PflFI
PinAI PmeI PshAI PshBI Psp1406I
PvuI SacII SalI SbfI SfuI
SgrAI SmaI SnaBI SpeI Sse8387I
SspI SwaI Tth111I XbaI XmaCI
XmaI
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# ONE CUT ENZYMES ( 36 enzymes) #
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AflIII 2618 ACACGT A^C[GA][CT]GT
BbrPI 787 CACGTG CAC^GTG
BbsI 1804 GTCTTC GAAGAC(2/6)
BbvCI 2670 GCTGAGG CCTCAGC(-5/-2)
BglII 2204 AGATCT A^GATCT
BpuAI 1804 GTCTTC GAAGAC(2/6)
BsaBI 2994 GATAGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiWI 2681 CGTACG C^GTACG
BsmBI 3583 GAGACG CGTCTC(1/5)
BsmI 2826 GCATTC GAATGC(1/-1)
BspHI 2873 TCATGA T^CATGA
BsrFI 170 GCCGGC [GA]^CCGG[CT]
BstAPI 1874 GCACTATCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 655 GGTGACC G^GT[ACGT]ACC
BstPI 655 GGTGACC G^GT[ACGT]ACC
Cfr10I 170 GCCGGC [GA]^CCGG[CT]
CpoI 2042 CGGTCCG CG^G[AT]CCG
EagI 2685 CGGCCG C^GGCCG
EclXI 2685 CGGCCG C^GGCCG
Eco52I 2685 CGGCCG C^GGCCG
EcoO65I 655 GGTGACC G^GT[ACGT]ACC
EcoRI 3379 GAATTC G^AATTC
MamI 2994 GATAGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MseI 2264 TTAA T^TAA
NaeI 170 GCCGGC GCC^GGC
NgoMIV 170 GCCGGC G^CCGGC
PmaCI 787 CACGTG CAC^GTG
PmlI 787 CACGTG CAC^GTG
RcaI 2873 TCATGA T^CATGA
RsrII 2042 CGGTCCG CG^G[AT]CCG
SapI 2883 GCTCTTC GCTCTTC(1/4)
ScaI 1178 AGTACT AGT^ACT
SexAI 737 ACCAGGT A^CC[AT]GGT
SfiI 2397 GGCCAAGGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SphI 995 GCATGC GCATG^C
Tru9I 2264 TTAA T^TAA
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# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AatII 1417 GACGTC GACGT^C
3585 GACGTC
AccI 1168 GTCTAC GT^[AC][GT]AC
2743 GTCTAC
AccIII 2945 TCCGGA T^CCGGA
3383 TCCGGA
Alw44I 75 GTGCAC G^TGCAC
1158 GTGCAC
AlwNI 2384 CAGGCACTG CAG[ACGT][ACGT][ACGT]^CTG
3244 CAGATCCTG
ApaLI 75 GTGCAC G^TGCAC
1158 GTGCAC
BamHI 2159 GGATCC G^GATCC
3272 GGATCC
BcgI 393 CGAAGGATGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2334 CGACGGGCCTGC
BclI 1091 TGATCA T^GATCA
1478 TGATCA
BsaAI 787 CACGTG [CT]AC^GT[GA]
1171 TACGTG
BsaI 1327 GAGACC GGTCTC(1/5)
2659 GAGACC
BsaWI 2945 TCCGGA [AT]^CCGG[AT]
3383 TCCGGA
BseAI 2945 TCCGGA T^CCGGA
3383 TCCGGA
BspEI 2945 TCCGGA T^CCGGA
3383 TCCGGA
BssSI 292 CTCGTG CACGAG(-5/-1)
1799 CTCGTG
FbaI 1091 TGATCA T^GATCA
1478 TGATCA
HincII 1132 GTCAAC GT[CT]^[GA]AC
2518 GTCAAC
HindII 1132 GTCAAC GT[CT]^[GA]AC
2518 GTCAAC
MroI 2945 TCCGGA T^CCGGA
3383 TCCGGA
NspI 995 GCATGC [GA]CATG^[CT]
1727 GCATGT
PaeR7I 2134 CTCGAG C^TCGAG
3462 CTCGAG
PflMI 786 CCACGTGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3573 CCACAGCCTGG
PvuII 3027 CAGCTG CAG^CTG
3043 CAGCTG
Van91I 786 CCACGTGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3573 CCACAGCCTGG
XhoI 2134 CTCGAG C^TCGAG
3462 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 121 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI ApoI Asp700I AspHI
AvaI AvaII AviII BalI BanI
BanII BbeI BbvI BcnI BglI
BmyI BpmI BsaHI BsaJI BseRI
BsgI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I Bsp1407I
BspMI BsrBI BsrDI BsrGI BsrI
BssKI BstF5I BstNI BstUI BstXI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI FspI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI KasI
Ksp632I MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MslI
MspA1I MspI MvaI MvnI MwoI
NarI NciI NcoI NdeII NlaIII
NlaIV PleI PpuMI PstI RsaI
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI SfoI SmlI SspBI StuI
StyI TaqI TfiI TseI Tsp45I
Tsp509I TspRI TthHB8I XcmI XhoII
XmnI