Restriction map for
pF1KE2504
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# NO CUT ENZYMES ( 98 enzymes) #
##################################
AclI AflII AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI AseI
AsnI AspEI AvrII BanII BbeI
BbrPI BbvCI BclI BfrI BglII
BlnI BsiEI BsiWI Bsp1407I BsrDI
BsrFI BsrGI BssSI Bst1107I BstBI
BstZ17I Bsu36I Cfr10I CpoI DraI
DraIII EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoT22I FbaI FseI
HgaI HpaI KasI KspI MfeI
MluI MunI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflMI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI Van91I XbaI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AccI 2899 GTAGAC GT^[AC][GT]AC
AccII 52 CGCG CG^CG
AccIII 98 TCCGGA T^CCGGA
AscI 50 GGCGCGCC GG^CGCGCC
Asp700I 1650 GAAGGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 1834 GAGCAC G[AT]GC[AT]^C
AspI 2695 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 1626 TGCGCA TGC^GCA
BciVI 1899 GTATCC GTATCC(6/5)
BfaI 451 CTAG C^TAG
BlpI 2100 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 2100 GCTGAGC GC^T[ACGT]AGC
BsaAI 369 TACGTG [CT]AC^GT[GA]
BsaBI 102 GATGGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 98 TCCGGA T^CCGGA
BsiHKAI 1834 GAGCAC G[AT]GC[AT]^C
BspEI 98 TCCGGA T^CCGGA
BspHI 1841 TCATGA T^CATGA
BspMI 2300 ACCTGC ACCTGC(4/8)
BssHII 51 GCGCGC G^CGCGC
BstEII 999 GGTAACC G^GT[ACGT]ACC
BstPI 999 GGTAACC G^GT[ACGT]ACC
BstUI 52 CGCG CG^CG
CelII 2100 GCTGAGC GC^T[ACGT]AGC
DrdI 2488 GACTTGGAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 1945 GAAGAG CTCTTC(1/4)
EcoO65I 999 GGTAACC G^GT[ACGT]ACC
EcoRI 1012 GAATTC G^AATTC
EcoRV 2503 GATATC GAT^ATC
FspI 1626 TGCGCA TGC^GCA
HindIII 697 AAGCTT A^AGCTT
Ksp632I 1945 GAAGAG CTCTTC(1/4)
MaeI 451 CTAG C^TAG
MamI 102 GATGGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 98 TCCGGA T^CCGGA
MvnI 52 CGCG CG^CG
NcoI 2054 CCATGG C^CATGG
PflFI 2695 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PstI 1714 CTGCAG CTGCA^G
RcaI 1841 TCATGA T^CATGA
Tth111I 2695 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmnI 1650 GAAGGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 33 enzymes) #
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AatII 940 GACGTC GACGT^C
1381 GACGTC
Acc65I 152 GGTACC G^GTACC
470 GGTACC
AcyI 940 GACGTC G[GA]^CG[CT]C
1381 GACGTC
AflIII 2666 ACATGT A^C[GA][CT]GT
2783 ACATGT
Asp718I 152 GGTACC G^GTACC
470 GGTACC
BalI 104 TGGCCA TGG^CCA
1935 TGGCCA
BcgI 522 CGAGTACAGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
857 GCATGGTGTTCG
BmyI 869 GTGCCC G[AGT]GC[ACT]^C
1834 GAGCAC
BsaHI 940 GACGTC G[GA]^CG[CT]C
1381 GACGTC
BsaI 2738 GAGACC GGTCTC(1/5)
2700 GGTCTC
BsaWI 98 TCCGGA [AT]^CCGG[AT]
986 ACCGGA
BsmBI 1383 CGTCTC CGTCTC(1/5)
2729 CGTCTC
BsmI 889 GAATGC GAATGC(1/-1)
2530 GAATGC
Bsp1286I 869 GTGCCC G[AGT]GC[ACT]^C
1834 GAGCAC
BspDI 2368 ATCGAT AT^CGAT
2500 ATCGAT
BspLU11I 2666 ACATGT A^CATGT
2783 ACATGT
BsrBI 2248 CCGCTC CCGCTC(-3/-3)
45 GAGCGG
BstXI 956 CCAAGTGTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2223 CCACGACTTTGG
ClaI 2368 ATCGAT AT^CGAT
2500 ATCGAT
DraII 1759 AGGTCCT [GA]G^G[ACGT]CC[CT]
2009 AGGACCT
DsaI 1091 CCGTGG C^C[GA][CT]GG
2054 CCATGG
EcoNI 2136 CCTCAGTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2616 CCTGTCCCAGG
EcoO109I 1759 AGGTCCT [GA]G^G[ACGT]CC[CT]
2009 AGGACCT
FauI 2640 CCCGC CCCGC(4/6)
47 GCGGG
HaeII 1084 AGCGCC [GA]GCGC^[CT]
2649 AGCGCC
Hin1I 940 GACGTC G[GA]^CG[CT]C
1381 GACGTC
KpnI 152 GGTACC GGTAC^C
470 GGTACC
MluNI 104 TGGCCA TGG^CCA
1935 TGGCCA
MscI 104 TGGCCA TGG^CCA
1935 TGGCCA
PpuMI 1759 AGGTCCT [GA]G^G[AT]CC[CT]
2009 AGGACCT
PvuII 901 CAGCTG CAG^CTG
1592 CAGCTG
SmlI 541 CTCAAG C^T[CT][GA]AG
1033 CTCAAG
TfiI 1606 GATTC G^A[AT]TC
2419 GATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 85 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI AvaI AvaII BamHI
BanI BbsI BbvI BcnI BglI
BpmI BpuAI BsaJI BseRI BsgI
BsiYI BslI BsmAI BsmFI BsoBI
BsrI BssKI BstAPI BstF5I BstNI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII EaeI EcoRII EcoT14I Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HincII HindII HinfI HpaII HphI
ItaI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV NspI PleI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
StyI TaqI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XcmI XhoII