Restriction map for
pF1KSDB0238
##################################
# NO CUT ENZYMES ( 82 enzymes) #
##################################
AatII AccI AclI AflII AgeI
AhdI Alw44I Aor51HI ApaLI AscI
AseI AsnI Asp700I AspEI AviII
AvrII BbvCI BclI BfrI BlnI
BlpI Bpu1102I BsaBI Bsp1407I BspDI
BspHI BspLU11I BsrGI BssHII Bst1107I
BstBI BstZ17I CelII ClaI CpoI
DraI Eam1105I Eco47III EcoRI EcoRV
EcoT22I FbaI FseI FspI HindIII
HpaI MamI MfeI MluI MunI
NaeI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PinAI PmeI PshBI Psp1406I
PvuI RcaI RsrII SalI SbfI
SfiI SfuI SgrAI SnaBI SpeI
Sse8387I SspBI SspI SwaI XbaI
XhoI XmnI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
Acc65I 2963 GGTACC G^GTACC
AccIII 500 TCCGGA T^CCGGA
AcsI 1951 GAATTT [GA]^AATT[CT]
ApoI 1951 GAATTT [GA]^AATT[CT]
Asp718I 2963 GGTACC G^GTACC
AspI 856 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 1109 GGATCC G^GATCC
BciVI 2539 GGATAC GTATCC(6/5)
BfaI 3766 CTAG C^TAG
BglII 524 AGATCT A^GATCT
BseAI 500 TCCGGA T^CCGGA
BsiWI 3015 CGTACG C^GTACG
BsmBI 3099 CGTCTC CGTCTC(1/5)
BsmI 1448 GCATTC GAATGC(1/-1)
BspEI 500 TCCGGA T^CCGGA
BsrDI 3719 GCAATG GCAATG(2/0)
BssSI 2276 CACGAG CACGAG(-5/-1)
BstAPI 41 GCAGCCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
EagI 2500 CGGCCG C^GGCCG
EclXI 2500 CGGCCG C^GGCCG
Eco52I 2500 CGGCCG C^GGCCG
HincII 2371 GTCAAC GT[CT]^[GA]AC
HindII 2371 GTCAAC GT[CT]^[GA]AC
KpnI 2963 GGTACC GGTAC^C
KspI 2535 CCGCGG CCGC^GG
MaeI 3766 CTAG C^TAG
MroI 500 TCCGGA T^CCGGA
NspI 966 GCATGC [GA]CATG^[CT]
PflFI 856 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 2115 GACTGTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 3216 GAGCTC GAGCT^C
SacII 2535 CCGCGG CCGC^GG
ScaI 1703 AGTACT AGT^ACT
SexAI 752 ACCTGGT A^CC[AT]GGT
SmaI 2089 CCCGGG CCC^GGG
SphI 966 GCATGC GCATG^C
Tsp509I 1952 AATT ^AATT
Tth111I 856 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 2089 CCCGGG C^CCGGG
XmaI 2089 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 14 enzymes) #
###################################
AflIII 20 ACGTGT A^C[GA][CT]GT
2330 ACACGT
AocI 2128 CCTGAGG CC^T[ACGT]AGG
2266 CCTCAGG
BbrPI 1611 CACGTG CAC^GTG
2199 CACGTG
BcgI 1343 GCAAGGCCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1574 GCAGCACAATCG
BsrBI 2209 CCGCTC CCGCTC(-3/-3)
547 GAGCGG
Bsu36I 2128 CCTGAGG CC^T[ACGT]AGG
2266 CCTCAGG
DrdI 676 GACCTCCGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1057 GACTGCCAAGTC
Eco81I 2128 CCTGAGG CC^T[ACGT]AGG
2266 CCTCAGG
EcoNI 2511 CCTGGCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2871 CCTGGATGAGG
HgaI 1478 GACGC GACGC(5/10)
1878 GACGC
PmaCI 1611 CACGTG CAC^GTG
2199 CACGTG
PmlI 1611 CACGTG CAC^GTG
2199 CACGTG
SapI 238 GAAGAGC GCTCTTC(1/4)
726 GCTCTTC
TfiI 1100 GAATC G^A[AT]TC
1149 GATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 122 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI ApaI AspHI AvaI
AvaII BalI BanI BanII BbeI
BbsI BbvI BcnI BglI BmyI
BpmI BpuAI BsaAI BsaHI BsaI
BsaJI BsaWI BseRI BsgI BsiEI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrFI BsrI
BssKI BstEII BstF5I BstNI BstPI
BstUI BstXI BstYI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DraIII DsaI EaeI EarI EcoO109I
EcoO65I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI KasI Ksp632I MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NarI NciI
NdeII NlaIII NlaIV PflMI PleI
PpuMI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SfoI
SmlI StuI StyI TaqI Tru9I
TseI Tsp45I TspRI TthHB8I Van91I
XcmI XhoII