Restriction map for
pF1KSDB0234
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# NO CUT ENZYMES ( 83 enzymes) #
##################################
AatII AccII AclI AflII AflIII
AgeI AhdI Aor51HI AscI AseI
AsnI AspEI AspI AviII AvrII
BcgI BfrI BglI BglII BlnI
BlpI Bpu1102I BsiEI BsiWI BsmBI
BspDI BspLU11I BsrBI BsrDI BssHII
BssSI BstBI BstUI BstXI CelII
ClaI CpoI DrdI EagI Eam1105I
EclXI Eco47III Eco52I FauI FseI
FspI HgaI KspI MfeI MluI
MspA1I MunI MvnI NheI NotI
NruI NspI NspV PacI PflFI
PinAI PmeI PshBI Psp1406I PvuI
PvuII RsrII SacI SacII SalI
SbfI SexAI SfiI SfuI SmaI
SnaBI SphI Sse8387I SwaI Tth111I
XbaI XmaCI XmaI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AcyI 298 GGCGCC G[GA]^CG[CT]C
Alw44I 3282 GTGCAC G^TGCAC
ApaLI 3282 GTGCAC G^TGCAC
Asp700I 3465 GAACCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BamHI 1860 GGATCC G^GATCC
BbeI 298 GGCGCC GGCGC^C
BbrPI 2884 CACGTG CAC^GTG
BbvCI 310 GCTGAGG CCTCAGC(-5/-2)
BcnI 2763 CCCGG CC^[GC]GG
BsaAI 2884 CACGTG [CT]AC^GT[GA]
BsaHI 298 GGCGCC G[GA]^CG[CT]C
BsgI 2985 GTGCAG GTGCAG(16/14)
Bsp1407I 525 TGTACA T^GTACA
BspMI 714 GCAGGT ACCTGC(4/8)
BsrGI 525 TGTACA T^GTACA
Bst1107I 2142 GTATAC GTA^TAC
BstAPI 3571 GCAGGAGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1029 GGTTACC G^GT[ACGT]ACC
BstPI 1029 GGTTACC G^GT[ACGT]ACC
BstZ17I 2142 GTATAC GTA^TAC
EcoO65I 1029 GGTTACC G^GT[ACGT]ACC
EcoRV 571 GATATC GAT^ATC
EcoT22I 2519 ATGCAT ATGCA^T
Hin1I 298 GGCGCC G[GA]^CG[CT]C
HpaI 2320 GTTAAC GTT^AAC
KasI 298 GGCGCC G^GCGCC
NaeI 2718 GCCGGC GCC^GGC
NarI 298 GGCGCC GG^CGCC
NciI 2763 CCCGG CC^[GC]GG
NcoI 2468 CCATGG C^CATGG
NdeI 305 CATATG CA^TATG
NgoMIV 2718 GCCGGC G^CCGGC
NsiI 2519 ATGCAT ATGCA^T
PaeR7I 1019 CTCGAG C^TCGAG
PflMI 3437 CCAGAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 2884 CACGTG CAC^GTG
PmlI 2884 CACGTG CAC^GTG
PshAI 3091 GACAGTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SapI 2948 GCTCTTC GCTCTTC(1/4)
ScaI 1765 AGTACT AGT^ACT
SfoI 298 GGCGCC GGC^GCC
SgrAI 2460 CGCCGGTG C[GA]^CCGG[CT]G
SpeI 2956 ACTAGT A^CTAGT
SspBI 525 TGTACA T^GTACA
StuI 3185 AGGCCT AGG^CCT
Van91I 3437 CCAGAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 3507 CCATTTCAACAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 1019 CTCGAG C^TCGAG
XmnI 3465 GAACCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 38 enzymes) #
###################################
Acc65I 802 GGTACC G^GTACC
2276 GGTACC
AccI 187 GTCTAC GT^[AC][GT]AC
2142 GTATAC
AccIII 2177 TCCGGA T^CCGGA
3192 TCCGGA
ApaI 2760 GGGCCC GGGCC^C
3015 GGGCCC
Asp718I 802 GGTACC G^GTACC
2276 GGTACC
AspHI 3282 GTGCAC G[AT]GC[AT]^C
3598 GAGCAC
AvaI 1019 CTCGAG C^[CT]CG[GA]G
3254 CCCGAG
BalI 601 TGGCCA TGG^CCA
2804 TGGCCA
BanII 2760 GGGCCC G[GA]GC[CT]^C
3015 GGGCCC
BbsI 1486 GAAGAC GAAGAC(2/6)
3451 GAAGAC
BciVI 399 GGATAC GTATCC(6/5)
232 GTATCC
BclI 374 TGATCA T^GATCA
467 TGATCA
BpuAI 1486 GAAGAC GAAGAC(2/6)
3451 GAAGAC
BsaBI 657 GATGAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2448 GATTGCCATC
BseAI 2177 TCCGGA T^CCGGA
3192 TCCGGA
BsiHKAI 3282 GTGCAC G[AT]GC[AT]^C
3598 GAGCAC
BsmI 789 GAATGC GAATGC(1/-1)
1530 GAATGC
BsoBI 1019 CTCGAG C^[CT]CG[GA]G
3254 CCCGAG
BspEI 2177 TCCGGA T^CCGGA
3192 TCCGGA
BspHI 2444 TCATGA T^CATGA
2688 TCATGA
DraIII 976 CACAAAGTG CAC[ACGT][ACGT][ACGT]^GTG
2881 CACCACGTG
DsaI 2084 CCGTGG C^C[GA][CT]GG
2468 CCATGG
EcoNI 41 CCTGTTTAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
505 CCTGACACAGG
EcoRI 1300 GAATTC G^AATTC
3172 GAATTC
EcoT14I 2468 CCATGG C^C[AT][AT]GG
2994 CCAAGG
FbaI 374 TGATCA T^GATCA
467 TGATCA
HindIII 1372 AAGCTT A^AGCTT
3412 AAGCTT
KpnI 802 GGTACC GGTAC^C
2276 GGTACC
MamI 657 GATGAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2448 GATTGCCATC
MluNI 601 TGGCCA TGG^CCA
2804 TGGCCA
MroI 2177 TCCGGA T^CCGGA
3192 TCCGGA
MscI 601 TGGCCA TGG^CCA
2804 TGGCCA
PstI 712 CTGCAG CTGCA^G
3084 CTGCAG
RcaI 2444 TCATGA T^CATGA
2688 TCATGA
SspI 442 AATATT AAT^ATT
1253 AATATT
StyI 2468 CCATGG C^C[AT][AT]GG
2994 CCAAGG
TaqI 1020 TCGA T^CGA
2660 TCGA
TthHB8I 1020 TCGA T^CGA
2660 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 88 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI AocI ApoI AvaII BanI
BbvI BfaI BmyI BpmI BsaI
BsaJI BsaWI BseRI BsiYI BslI
BsmAI BsmFI Bsp1286I BsrFI BsrI
BssKI BstF5I BstNI BstYI Bsu36I
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraI DraII EaeI EarI
Eco81I EcoO109I EcoRII Fnu4HI FokI
HaeII HaeIII HapII HhaI HinP1I
HincII HindII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspI MvaI MwoI NdeII
NlaIII NlaIV PleI PpuMI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI XhoII