Restriction map for
pF1KSDB0060
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# NO CUT ENZYMES ( 92 enzymes) #
##################################
AatII AccIII AflII AgeI Alw44I
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp700I AviII AvrII
BalI BbrPI BfrI BglI BglII
BlnI BsaAI BsaWI BseAI BsiEI
BsiWI Bsp1407I BspEI BsrFI BsrGI
BssHII BstBI BstEII BstPI BstXI
Bsu36I Cfr10I CpoI DsaI EaeI
EagI EclXI Eco47III Eco52I Eco81I
EcoO65I EcoRV FseI FspI HindIII
HpaI KspI MluI MluNI MroI
MscI NaeI NcoI NdeI NgoMIV
NheI NotI NruI NspV PacI
PinAI PmaCI PmeI PmlI PshBI
PvuI RsrII SacII SalI SapI
SbfI ScaI SexAI SfiI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SwaI XcmI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 57 enzymes) #
###################################
AccI 1389 GTATAC GT^[AC][GT]AC
AclI 28 AACGTT AA^CGTT
AcyI 1094 GGCGCC G[GA]^CG[CT]C
AflIII 2051 ACATGT A^C[GA][CT]GT
AhdI 13 GACCAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 13 GACCAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 1958 CTCGAG C^[CT]CG[GA]G
BamHI 56 GGATCC G^GATCC
BbeI 1094 GGCGCC GGCGC^C
BciVI 711 GGATAC GTATCC(6/5)
BlpI 291 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 291 GCTGAGC GC^T[ACGT]AGC
BsaBI 2227 GATTTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 1094 GGCGCC G[GA]^CG[CT]C
BsmBI 2403 GAGACG CGTCTC(1/5)
BsmI 2358 GAATGC GAATGC(1/-1)
BsoBI 1958 CTCGAG C^[CT]CG[GA]G
BspHI 1491 TCATGA T^CATGA
BspLU11I 2051 ACATGT A^CATGT
BspMI 1694 ACCTGC ACCTGC(4/8)
BsrBI 942 CCGCTC CCGCTC(-3/-3)
Bst1107I 1389 GTATAC GTA^TAC
BstAPI 205 GCAGATGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstZ17I 1389 GTATAC GTA^TAC
CelII 291 GCTGAGC GC^T[ACGT]AGC
DraIII 2251 CACGAGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 865 GACACAGCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 13 GACCAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 297 CCTCAGTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 1796 GAATTC G^AATTC
EcoT14I 1164 CCAAGG C^C[AT][AT]GG
EcoT22I 1583 ATGCAT ATGCA^T
HaeII 1094 GGCGCC [GA]GCGC^[CT]
HgaI 1552 GACGC GACGC(5/10)
Hin1I 1094 GGCGCC G[GA]^CG[CT]C
HincII 1930 GTTGAC GT[CT]^[GA]AC
HindII 1930 GTTGAC GT[CT]^[GA]AC
KasI 1094 GGCGCC G^GCGCC
MamI 2227 GATTTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1940 CAATTG C^AATTG
MslI 795 CATCAAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 1940 CAATTG C^AATTG
NarI 1094 GGCGCC GG^CGCC
NsiI 1583 ATGCAT ATGCA^T
PaeR7I 1958 CTCGAG C^TCGAG
PflMI 2223 CCAGGATTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 2608 GACGAGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 28 AACGTT AA^CGTT
PvuII 1193 CAGCTG CAG^CTG
RcaI 1491 TCATGA T^CATGA
SacI 456 GAGCTC GAGCT^C
SfoI 1094 GGCGCC GGC^GCC
SspI 2544 AATATT AAT^ATT
StyI 1164 CCAAGG C^C[AT][AT]GG
Van91I 2223 CCAGGATTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 1800 TCTAGA T^CTAGA
XhoI 1958 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
Acc65I 1691 GGTACC G^GTACC
2692 GGTACC
AccII 519 CGCG CG^CG
2680 CGCG
AlwNI 40 CAGAACCTG CAG[ACGT][ACGT][ACGT]^CTG
206 CAGATGCTG
Asp718I 1691 GGTACC G^GTACC
2692 GGTACC
AspHI 456 GAGCTC G[AT]GC[AT]^C
2529 GTGCTC
AspI 690 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1527 GACCAAGTC
BanII 456 GAGCTC G[GA]GC[CT]^C
2668 GAGCCC
BbvCI 1190 CCTCAGC CCTCAGC(-5/-2)
1254 CCTCAGC
BcgI 26 CGAACGTTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
119 GCAGCAACTTCG
BclI 1494 TGATCA T^GATCA
2337 TGATCA
BcnI 9 CCCGG CC^[GC]GG
2093 CCGGG
BsiHKAI 456 GAGCTC G[AT]GC[AT]^C
2529 GTGCTC
BspDI 1007 ATCGAT AT^CGAT
1404 ATCGAT
BssSI 2251 CACGAG CACGAG(-5/-1)
1261 CTCGTG
BstUI 519 CGCG CG^CG
2680 CGCG
ClaI 1007 ATCGAT AT^CGAT
1404 ATCGAT
DraI 2313 TTTAAA TTT^AAA
2506 TTTAAA
DraII 305 AGGACCC [GA]G^G[ACGT]CC[CT]
1445 AGGTCCT
EcoO109I 305 AGGACCC [GA]G^G[ACGT]CC[CT]
1445 AGGTCCT
FbaI 1494 TGATCA T^GATCA
2337 TGATCA
KpnI 1691 GGTACC GGTAC^C
2692 GGTACC
MvnI 519 CGCG CG^CG
2680 CGCG
NciI 9 CCCGG CC^[GC]GG
2093 CCGGG
PflFI 690 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1527 GACCAAGTC
PpuMI 305 AGGACCC [GA]G^G[AT]CC[CT]
1445 AGGTCCT
StuI 359 AGGCCT AGG^CCT
403 AGGCCT
TfiI 1410 GATTC G^A[AT]TC
2512 GAATC
Tth111I 690 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1527 GACCAAGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 81 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AvaII BanI BbsI BbvI
BfaI BmyI BpmI BpuAI BsaI
BsaJI BseRI BsgI BsiYI BslI
BsmAI BsmFI Bsp1286I BsrDI BsrI
BssKI BstF5I BstNI BstYI Cac8I
CfoI Cfr13I DdeI DpnII EarI
EcoRII FauI Fnu4HI FokI HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MspA1I MspI MvaI MwoI
NdeII NlaIII NlaIV NspI PleI
PstI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI TaqI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII