Restriction map for
pF1KSDB0042
##################################
# NO CUT ENZYMES ( 119 enzymes) #
##################################
AatII AccII AclI AcsI AcyI
AflII AflIII AgeI AhdI Alw44I
ApaI ApaLI ApoI AscI AseI
AsnI Asp700I AspEI AspI AvaI
AviII AvrII BamHI BbeI BbrPI
BclI BfrI BglI BlnI BlpI
Bpu1102I BsaAI BsaHI BsaI BsgI
BsiWI BsmI BsoBI Bsp1407I BspDI
BspHI BspLU11I BsrFI BsrGI BssHII
BssSI Bst1107I BstBI BstUI BstZ17I
CelII Cfr10I ClaI CpoI DraI
DsaI EagI Eam1105I EclXI Eco52I
EcoRI EcoRV EcoT22I FbaI FseI
FspI Hin1I HincII HindII HpaI
KasI KspI MfeI MluI MunI
MvnI NaeI NarI NcoI NgoMIV
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I RcaI
RsrII SacII SalI SapI SbfI
ScaI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI Sse8387I SspBI
SspI SwaI Tsp509I Tth111I XbaI
XhoI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
Acc65I 292 GGTACC G^GTACC
AccI 1747 GTCTAC GT^[AC][GT]AC
AccIII 83 TCCGGA T^CCGGA
AocI 795 CCTCAGG CC^T[ACGT]AGG
Aor51HI 1172 AGCGCT AGC^GCT
Asp718I 292 GGTACC G^GTACC
AspHI 1579 GAGCTC G[AT]GC[AT]^C
BbvCI 1281 GCTGAGG CCTCAGC(-5/-2)
BciVI 1657 GGATAC GTATCC(6/5)
BsaBI 1023 GATGAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 83 TCCGGA T^CCGGA
BsiEI 1010 CGATCG CG[GA][CT]^CG
BsiHKAI 1579 GAGCTC G[AT]GC[AT]^C
BsmBI 1276 GAGACG CGTCTC(1/5)
BspEI 83 TCCGGA T^CCGGA
BspMI 429 ACCTGC ACCTGC(4/8)
BsrBI 520 GAGCGG CCGCTC(-3/-3)
BstEII 1543 GGTGACC G^GT[ACGT]ACC
BstPI 1543 GGTGACC G^GT[ACGT]ACC
BstXI 1948 CCAGGGTCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 795 CCTCAGG CC^T[ACGT]AGG
DraII 1318 AGGACCC [GA]G^G[ACGT]CC[CT]
DrdI 1642 GACAGCTTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 1172 AGCGCT AGC^GCT
Eco81I 795 CCTCAGG CC^T[ACGT]AGG
EcoO109I 1318 AGGACCC [GA]G^G[ACGT]CC[CT]
EcoO65I 1543 GGTGACC G^GT[ACGT]ACC
HaeII 1172 AGCGCT [GA]GCGC^[CT]
HgaI 1278 GACGC GACGC(5/10)
HindIII 1405 AAGCTT A^AGCTT
KpnI 292 GGTACC GGTAC^C
MaeII 1692 ACGT A^CGT
MamI 1023 GATGAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 83 TCCGGA T^CCGGA
NheI 969 GCTAGC G^CTAGC
NspI 983 GCATGC [GA]CATG^[CT]
PpuMI 1318 AGGACCC [GA]G^G[AT]CC[CT]
PvuI 1010 CGATCG CGAT^CG
SacI 1579 GAGCTC GAGCT^C
SexAI 254 ACCTGGT A^CC[AT]GGT
SmlI 704 CTCAAG C^T[CT][GA]AG
SphI 983 GCATGC GCATG^C
TaqI 1903 TCGA T^CGA
TthHB8I 1903 TCGA T^CGA
XcmI 1994 CCAAGCAGAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AlwNI 1111 CAGAGTCTG CAG[ACGT][ACGT][ACGT]^CTG
1999 CAGAAGCTG
BanI 194 GGTGCC G^G[CT][GA]CC
292 GGTACC
BcgI 1466 CGACTGGCTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1341 GCATCATGGTCG
BcnI 191 CCCGG CC^[GC]GG
1809 CCGGG
BfaI 946 CTAG C^TAG
970 CTAG
BglII 828 AGATCT A^GATCT
1770 AGATCT
BsaWI 83 TCCGGA [AT]^CCGG[AT]
1547 ACCGGA
BseRI 996 CTCCTC GAGGAG(10/8)
34 GAGGAG
BstAPI 305 GCAAGGTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1929 GCAGAAGATGC
DraIII 253 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
943 CACCTAGTG
EarI 76 GAAGAG CTCTTC(1/4)
913 GAAGAG
EcoNI 1371 CCTTGGAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1534 CCTGTTGGAGG
FauI 1217 CCCGC CCCGC(4/6)
1248 GCGGG
Ksp632I 76 GAAGAG CTCTTC(1/4)
913 GAAGAG
MaeI 946 CTAG C^TAG
970 CTAG
MseI 773 TTAA T^TAA
1259 TTAA
MspA1I 617 CAGCTG C[AC]G^C[GT]G
1144 CAGCTG
NciI 191 CCCGG CC^[GC]GG
1809 CCGGG
NdeI 1184 CATATG CA^TATG
1780 CATATG
PflMI 1110 CCAGAGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1140 CCATCAGCTGG
PvuII 617 CAGCTG CAG^CTG
1144 CAGCTG
StuI 799 AGGCCT AGG^CCT
894 AGGCCT
Tru9I 773 TTAA T^TAA
1259 TTAA
Van91I 1110 CCAGAGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1140 CCATCAGCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 70 enzymes) #
#########################################
AciI AfaI AluI AlwI AvaII
BalI BanII BbsI BbvI BmyI
BpmI BpuAI BsaJI BsiYI BslI
BsmAI BsmFI Bsp1286I BsrDI BsrI
BssKI BstF5I BstNI BstYI Cac8I
CfoI Cfr13I DdeI DpnII EaeI
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI MaeIII MboI MboII
MflI MluNI MnlI MscI MslI
MspI MvaI MwoI NdeII NlaIII
NlaIV PleI PstI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI StyI
TfiI TseI Tsp45I TspRI XhoII